FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410880
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA31430.7649435358255452No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19850.48310942367601245No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT13150.3200447819314642No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11000.2677180685358255No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT10900.2652842679127726No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10710.260660046728972No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC10650.2591997663551402No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG10010.24362344236760125No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC9930.24167640186915887No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9890.2407028816199377No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9460.23023753894080998No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9160.22293613707165108No Hit
CTCTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8570.20857671339563866No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGGAT8290.20176207165109034No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT7910.19251362928348908No Hit
AGATAAGGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT7820.19032320872274142No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7690.1871592679127726No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7680.1869158878504673No Hit
ACTTTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATAT7250.17645054517133957No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCGGTACG7200.17523364485981308No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7070.17206970404984426No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG6980.1698792834890966No Hit
CAGTAAATGAGAAACGAAGATTGCAATTAATTATGCCTGCTAGGTTTTAT6780.16501168224299065No Hit
CTCATAAAGTGGGTAACTTTACGGGGCTTTATTCTTCTACTTTACCTGTC6460.15722352024922118No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC6440.1567367601246106No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATT6430.1564933800623053No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC6050.14724493769470404No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA6020.14651479750778817No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTC5850.14237733644859812No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5840.14213395638629284No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG5780.14067367601246106No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5750.13994353582554517No Hit
CTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAT5670.1379964953271028No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC5630.1370229750778816No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT5620.13677959501557632No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5580.13580607476635514No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5470.1331288940809969No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5430.1321553738317757No Hit
AGTCTGTACGGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5310.12923481308411217No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5240.12753115264797507No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5180.1260708722741433No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAATCTTCGTTTCTCA5160.12558411214953272No Hit
GTAAATGAGAAACGAAGATTGCAATTAATTATGCCTGCTAGGTTTTATCC5130.12485397196261683No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAAT5100.12412383177570092No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5090.12388045171339564No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5050.12290693146417445No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA5030.12242017133956386No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT5010.12193341121495328No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA5000.12169003115264797No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4950.12047313084112149No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA4910.11949961059190033No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4890.11901285046728971No Hit
TCCTTGGACTCATAAAGTGGGTAACTTTACGGGGCTTTATTCTTCTACTT4880.11876947040498442No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGTTGCCT4840.11779595015576323No Hit
GTCCCGCGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC4790.11657904984423675No Hit
GTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGTATGATGTGAT4770.11609228971962618No Hit
GTAATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4730.11511876947040499No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4730.11511876947040499No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATTA4730.11511876947040499No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC4700.11438862928348908No Hit
CAATAGGTCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT4690.1141452492211838No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGCG4570.11122468847352025No Hit
GGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAG4540.11049454828660435No Hit
GGCATAATTAATTGCAATCTTCGTTTCTCATTTACTGTGAGTGGGCCTAC4510.10976440809968849No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT4510.10976440809968849No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4450.1083041277258567No Hit
ATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATCTTCAACCACC4400.1070872274143302No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT4390.10684384735202491No Hit
GGTTTGTATTATGCCCTGGGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT4370.10635708722741433No Hit
GAATACAGGCATACAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA4370.10635708722741433No Hit
GTATTATGCCCTGGGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT4320.10514018691588785No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA4310.10489680685358256No Hit
TCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTACCGAAGGAAA4310.10489680685358256No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT4300.10465342679127725No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGG4270.10392328660436137No Hit
CTCTAAGGCATCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA4260.10367990654205608No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4250.10343652647975077No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACGGCATCTTGAGTCCCTTTTTACC4240.10319314641744548No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAATCTTCGTTTCTCATTTAC4230.10294976635514018No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA4160.10124610591900313No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4160.10124610591900313No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA4150.10100272585669781No Hit
GCTTTATTCTTCTACTTTACCTGTCTTTAACCCTCATTGGAAAACACCCT4120.10027258566978194No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCTAGGCAACGGCCAGGTC4120.10027258566978194No Hit
CCTTTACCCCGTTGCTAGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4120.10027258566978194No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)