FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410880
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA29290.7128602024922118No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19790.48164914330218067No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT13490.32831970404984423No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG12720.30957943925233644No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC10980.26723130841121495No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10910.2655276479750779No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC10620.25846962616822433No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10380.2526285046728972No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10250.24946456386292834No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9930.24167640186915887No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCAT9810.23875584112149534No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9230.22463979750778815No Hit
CTCTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8800.2141744548286604No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGGAT8760.21320093457943926No Hit
AGATAAGGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT7980.19421728971962618No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTGTATGCCTGTATT7910.19251362928348908No Hit
ACTTTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATAT7470.18180490654205606No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT7210.17547702492211836No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7160.1742601246105919No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7140.1737733644859813No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6900.1679322429906542No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC6740.16403816199376947No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG6570.15990070093457945No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCGGTACG6510.15844042056074764No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA6050.14724493769470404No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5950.14481113707165108No Hit
CAGTAAATGAGAAACGAAGATTGCAATTAATTATGCCTGCTAGGTTTTAT5860.1426207165109034No Hit
CTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAT5800.14116043613707163No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT5770.14043029595015577No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC5770.14043029595015577No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5750.13994353582554517No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC5650.1375097352024922No Hit
CTCATAAAGTGGGTAACTTTACGGGGCTTTATTCTTCTACTTTACCTGTC5630.1370229750778816No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5560.13531931464174457No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5550.13507593457943926No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5450.1326421339563863No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAATCTTCGTTTCTCA5400.1314252336448598No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTC5370.13069509345794394No Hit
TCCTTGGACTCATAAAGTGGGTAACTTTACGGGGCTTTATTCTTCTACTT5220.1270443925233645No Hit
AGTCTGTACGGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5190.1263142523364486No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA5190.1263142523364486No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA5160.12558411214953272No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5080.12363707165109035No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5040.12266355140186916No Hit
GTAAATGAGAAACGAAGATTGCAATTAATTATGCCTGCTAGGTTTTATCC5010.12193341121495328No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACGGCATCTTGAGTCCCTTTTTACC4970.12095989096573209No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG4960.1207165109034268No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA4880.11876947040498442No Hit
GTAATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4770.11609228971962618No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT4760.11584890965732088No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGCGGGGTAGGTTGCCT4630.11268496884735202No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4580.11146806853582554No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC4580.11146806853582554No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT4560.11098130841121497No Hit
GTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGTATGATGTGAT4560.11098130841121497No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAATCTTCGTTTCTCATTTAC4500.10952102803738317No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT4500.10952102803738317No Hit
GTATTATGCCCTGGGCCTGAGGGCTCCACCCCAAAAGGCCTCCGTGCGGT4500.10952102803738317No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTACT4490.10927764797507787No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCTAGGCAACGGCCAGGTC4480.10903426791277258No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4470.1087908878504673No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4460.108547507788162No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACA4440.10806074766355139No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATTA4440.10806074766355139No Hit
CCTTTACCCCGTTGCTAGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4360.10611370716510904No Hit
CTCTAAGGCATCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA4350.10587032710280372No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGG4320.10514018691588785No Hit
CAATAGGTCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT4220.10270638629283489No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGCG4220.10270638629283489No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA4190.101976246105919No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA4170.10148948598130841No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4160.10124610591900313No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4160.10124610591900313No Hit
GGTTTGTATTATGCCCTGGGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT4160.10124610591900313No Hit
TCTCGGGGCCGCTTGGGGATCTTTCGTCCCCTTCTCCGTCTGCCGTTTCG4130.10051596573208722No Hit
GTTATGGGTCATTGCCACAAGATCACATCATACAGAAAATCAAAGAATGT4120.10027258566978194No Hit
CTCCGAAAGACACCAAATACTCTATTACTGTTTCTCTTCCAAAAGTGAGA4120.10027258566978194No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)