FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437426
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25440.5815840850795334No Hit
TCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA15070.34451541517879597No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCCACACTTCCGGA11930.27273184492919944No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCTCTAAATTTTAT11820.27021713386950025No Hit
GTATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGA10290.23523978913004714No Hit
GTGTAAAAGGAGCAGCAAAACCCAAAAGGCCCACAATTCGTTGACACACT10150.232039247781339No Hit
TTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9660.22083735306086058No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8800.20117688477593923No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA8380.19157526072981487No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTCTTGTGGTTTCACATTTCTT8080.18471695783972603No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTAT7950.18174502658735422No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7680.17557255398627425No Hit
GCCCTAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC7380.16871425109618543No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7230.165285099651141No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT7160.16368482897678693No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT7130.16299899868777806No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAAGAGAGGTGC6880.1572837462793707No Hit
GTATTTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGA6870.15705513618303438No Hit
CTAGCTACCTGGGTGGGTGTTAATTTGGAAGATCCAGCATCTAGGGACCT6870.15705513618303438No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6860.1568265260866981No Hit
ATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTCACGGTGGTTTCCATGC6780.15499764531600774No Hit
TCTAAATACCGCCTCAGCGCTGTATCGGGATGCCTTAGAATCTCCTGAAC6730.15385459483432626No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTGATTTTGTGT6610.15111127367829072No Hit
GGGCAAGATTTGGTGGGCGTTCACGGTGGTTTCCATGCGACGTGCAGAGG6490.1483679525222552No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC6430.1469962919442374No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT6300.14402436069186558No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT6280.143567140499193No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6210.14196686982483894No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC6150.14059520924682117No Hit
CATCATACACAAAATCAAAGACTGTTTTAGAAAACTTCCTGTTAACAGGC6090.13922354866880343No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC6020.13762327799444934No Hit
GGATAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGGCCCACAAT5870.1341941265494049No Hit
CAACAGGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTT5870.1341941265494049No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5710.1305363650080242No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5660.12939331452634276No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5610.12825026404466128No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5500.12573555298496203No Hit
TCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA5480.12527833279228942No Hit
GTGTATGATGTGTTCTTGTGGCAATGATCCATAACTTCCAATGACATAAC5480.12527833279228942No Hit
CCCTTATAAAGAATTTGGAGCTACCGTGGAGTTACTCTCGTTTTTGCCTT5460.12482111259961685No Hit
ACCGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG5370.12276362173259019No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG5370.12276362173259019No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA5330.12184918134724501No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG5200.1188772500948732No Hit
GTTATGGATCATTGCCACAAGAACACATCATACACAAAATCAAAGACTGT5180.1184200299022006No Hit
ACTCCTGCTCAAGGAACCTCTATGTATCCCTCATGTTGCTGTACCAAACC5090.11636253903517396No Hit
TTCTAAAACAGTCTTTGATTTTGTGTATGATGTGTTCTTGTGGCAATGAT5090.11636253903517396No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA5070.11590531884250135No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACAGTCTTTG5060.11567670874616508No Hit
CCTCTATGTATCCCTCATGTTGCTGTACCAAACCTTCGGACGGAAATTGC5010.11453365826448358No Hit
GTATCGGGATGCCTTAGAATCTCCTGAACATTGTTCACCTCACCATACTG5000.1143050481681473No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4900.11201894720478435No Hit
CTCCGAAAGACACCAAATATTCTATAACTGTTTCTCTTCCAAAAGTGAGA4890.11179033710844806No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC4810.10996145633775771No Hit
ATAGAGTCCCAAGCGACCCCGAGAAGGGTCGTCCGCGGGATTCAGCTCCG4670.10676091498904958No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACCGTGG4660.10653230489271329No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGAGC4660.10653230489271329No Hit
GTATGGATCGGCAGAGGAGCCCTAAAGGTTCCACGCATGCGCTGATGGCC4650.10630369479637698No Hit
TCTAGATACCGCCTCAGCGCTGTATCGGGATGCCTTAGAATCTCCTGAAC4600.10516064431469552No Hit
ACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTGACGCA4600.10516064431469552No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC4590.10493203421835923No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTATT4580.10470342412202292No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4580.10470342412202292No Hit
TAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGGCCCACAATTCG4560.10424620392935034No Hit
CTTTATACCGCTGTGACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA4550.10401759383301404No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT4550.10401759383301404No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGGCCGCGTAAA4510.10310315344766885No Hit
ATCAAAGACTGTTTTAGAAAACTTCCTGTTAACAGGCCTATTGATTGGAA4440.10150288277331479No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATAA4410.10081705248430593No Hit
ACCTTGAGGCATACTTCAAAGACTGTTTGTTTAAAGACTGGGAGGAGTTG4380.10013122219529703No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)