FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435303
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA28430.6531082946820951No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT13370.30714238128384136No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12240.2811834515268675No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12200.28026455135847905No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC10480.24075184411777545No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT9480.21777933990806403No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT9360.21502263940289867No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA9240.21226593889773332No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT8970.20606336276111126No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8930.20514446259272276No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8900.20445528746643143No Hit
GTATAATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC8720.2003202367086834No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8580.19710408611932376No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8560.19664463603512955No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC8270.18998260981431325No Hit
CCTCCAGCGTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7740.17780718258316622No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7740.17780718258316622No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7650.1757396572042922No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC7560.17367213182541816No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT7560.17367213182541816No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT7410.17022625619396145No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAG7280.16723983064669898No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7250.16655065552040763No Hit
ATGTAAGACCTTGGGCAAGAAGTGATGGGCGTTCACGGTGGTCTCCATGC7090.1628750548468538No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7010.1610372545100769No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC6920.15896972913120286No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT6770.15552385349974615No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGATGGGCGTTC6650.1527671529945808No Hit
GCCTAGCAGCCATGGATACGATGTATATTTGCGGGAGAGAACAACAGAGT6600.15161852778409524No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6470.14863210223683274No Hit
CTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTT6450.1481726521526385No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT6350.14587540173166738No Hit
ACATTATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTCACAGG6330.14541595164747315No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6320.14518622660537603No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA6270.14403760139489044No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6240.14334842626859912No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6180.14197007601601644No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6110.14036200072133664No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT6020.1382944753424626No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGTGA5810.13347024945842323No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT5650.12979464878486938No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5600.12864602357438382No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5550.12749739836389826No Hit
CAATAGGCCTGTGAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5520.1268082232376069No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATTTTT5460.12542987298502423No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTAATTATGCCTGCTAGGTT5450.1252001479429271No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC5440.12497042290082999No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC5400.12405152273244154No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5400.12405152273244154No Hit
GTATGGATCGGCAGGGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC5370.1233623476061502No Hit
AATATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5360.12313262256405308No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG5340.12267317247985886No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5310.12198399735356752No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT5180.11899757180630505No Hit
ATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTT5060.11624087130113966No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA5050.11601114625904255No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5030.11555169617484833No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5030.11555169617484833No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4960.11394362088016853No Hit
CATGTATTCAGTCTAAGCAGGCTTTCACTTTCTCACCAACTTACAAGGCC4950.11371389583807141No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG4940.11348417079597431No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA4910.11279499566968297No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTA4890.11233554558548872No Hit
ATCCAATATCCAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGC4840.11118692037500315No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4810.11049774524871181No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4760.10934912003822625No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTGTGCTGGGGGGAACTAATGA4730.10865994491193491No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCTTTTCTCA4720.1084302198698378No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4700.10797076978564357No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4700.10797076978564357No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT4620.10613296944886666No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4620.10613296944886666No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4580.10521406928047819No Hit
GTCAATGAGAAAAGAAGACTGCAATTAATTATGCCTGCTAGGTTTTATCC4440.1019979186911186No Hit
GTATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTG4420.10153846860692438No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT4400.10107901852273016No Hit
GAAAAGAAGACTGCAATTAATTATGCCTGCTAGGTTTTATCCAAATGTTA4380.10061956843853592No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA4370.1003898433964388No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA4360.1001601183543417No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)