FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435303
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA27210.6250818395462471No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12220.2807240014426733No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC12150.27911592614799347No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT11830.2717647248008858No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10820.2485624955490773No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG10750.2469544202543975No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10500.24121129420196968No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10180.233860092854862No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC10090.231792567475988No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9480.21777933990806403No Hit
GTATAATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC8960.2058336377190141No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT8880.2039958373822372No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT8850.20330666225594585No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8470.19457711065625552No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT8210.18860425956173055No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT8130.18676645922495366No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT7790.17895580779365178No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC7780.17872608275155466No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT7450.17114515636234992No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7340.16861818089928166No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT7120.16356422997314513No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC7080.16264532980475668No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7030.16149670459427112No Hit
CCTCCAGCGTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6990.16057780442588265No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG6940.1594291792153971No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA6870.1578211039207173No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6860.15759137887862018No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT6640.15253742795248368No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT6150.1412809008897251No Hit
GCCTAGCAGCCATGGATACGATGTATATTTGCGGGAGAGAACAACAGAGT6140.14105117584762797No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC6120.14059172576343376No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGTGA6070.13944310055294817No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT6060.13921337551085106No Hit
AATATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG6040.13875392542665685No Hit
ATGTAAGACCTTGGGCAAGAAGTGATGGGCGTTCACGGTGGTCTCCATGC5900.13553777483729723No Hit
ACATTATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTCACAGG5900.13553777483729723No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGATGGGCGTTC5890.1353080497952001No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT5830.13392969954261744No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5780.13278107433213188No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5670.1302540988690636No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA5640.1295649237427723No Hit
CTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTT5570.1279568484480925No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC5570.1279568484480925No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC5540.12726767332180114No Hit
CAATAGGCCTGTGAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5220.1199164719746935No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGA5140.11807867163791658No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT5120.11761922155372234No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5040.11578142121694544No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5030.11555169617484833No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATTTTT4990.11463279600645987No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4970.11417334592226565No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4950.11371389583807141No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAG4940.11348417079597431No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4930.11325444575387719No Hit
GTATGGATCGGCAGGGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC4930.11325444575387719No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA4920.11302472071178007No Hit
CATGTATTCAGTCTAAGCAGGCTTTCACTTTCTCACCAACTTACAAGGCC4900.11256527062758585No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4850.11141664541710027No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA4820.11072747029080893No Hit
ATCCAATATCCAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGC4780.10980857012242047No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT4680.10751131970144934No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCTTTTCTCA4560.10475461919628395No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTG4540.10429516911208973No Hit
ATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTT4520.10383571902789551No Hit
GTCTAAGCAGGCTTTCACTTTCTCACCAACTTACAAGGCCTTTCTGTGTA4510.1036059939857984No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA4450.10222764373321572No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT4440.1019979186911186No Hit
CTCACAGTCAATGAGAAAAGAAGACTGCAATTAATTATGCCTGCTAGGTT4430.1017681936490215No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4420.10153846860692438No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC4380.10061956843853592No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA4370.1003898433964388No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)