FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479794
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTAACCTTACCAAATATTTGCCCTTGGAAAAAGGCATTAAACCTTAT19970.4162202945430747No Hit
ATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTT16150.3366027920315802No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG12800.26678116024793974No Hit
ACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAG12710.26490535521494646No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA12560.26177901349329086No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11080.23093244183962283No Hit
GTATGTAAATAATGCATAGTTTTGAAGTAATGATTAACTGCATGTTCAGG10960.22843136846229842No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT10050.20946489535092144No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG10010.2086312042251466No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA9950.2073806675364844No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA9910.2065469764107096No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC9850.2052964397220474No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9640.2009195613117296No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC9620.2005027157488422No Hit
ACCAAAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAG9620.2005027157488422No Hit
CATGTATACAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9620.2005027157488422No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT9250.1927910728354252No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9090.18945630833232593No Hit
ACTATGCATTATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAG8750.18236993376324007No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG8480.17674251866426008No Hit
CTATTAATAATGTCTTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG8460.17632567310137268No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA8430.17570040475704157No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8430.17570040475704157No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8370.17444986806837937No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8040.16757191628073714No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA8000.16673822515496234No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7970.16611295681063123No Hit
GGATAATAAGGTTTAATGCCTTTTTCCAAGGGCAAATATTTGGTAAGGTT7780.16215292396320086No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7730.16111081005598238No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7700.16048554171165125No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7640.15923500502298904No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCTTCCTGT7470.1556918177384461No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7470.1556918177384461No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC7460.1554833949570024No Hit
CTCTTTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA7400.15423285826834016No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7350.15319074436112165No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGAAAAAGGCATTAAAC7350.15319074436112165No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7040.14672963813636686No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT7030.14652121535492316No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA7020.14631279257347946No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC7020.14631279257347946No Hit
GTCCCGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA6890.1436032964147113No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6870.1431864508518239No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6840.14256118250749278No Hit
GTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT6660.13880957244150616No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6460.13464111681263208No Hit
CTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA6440.13422427124974468No Hit
GGGTAAAGGTGCATGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA6310.13151477509097653No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC6310.13151477509097653No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6240.13005581562087062No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC6230.12984739283942692No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG6210.12943054727653952No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG6050.12609578277344027No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT5990.12484524608477804No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5980.12463682330333435No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5960.12421997774044694No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATA5920.12338628661467213No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5830.1215104815816788No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5790.120676790455904No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5760.12005152211157288No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5750.11984309933012918No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5710.11900940820435436No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5550.11567464370125513No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5490.1144241070125929No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5420.11296514754248697No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC5330.11108934250949366No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTTTCCAAGGGC5320.11088091972804995No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA5300.11046407416516256No Hit
GGAACATATTGTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA5280.11004722860227514No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5200.1083798463507255No Hit
ATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5140.1071293096620633No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5120.10671246409917591No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5120.10671246409917591No Hit
ATACAGCACTCAGGCAAGCTATTCTCTGTTGGGGTGAGTTGATGAATCTG5120.10671246409917591No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5060.10546192741051368No Hit
ATGTAAGACCTTGGGCAAGACCTGATGGGCGTTCACGGTGGTCTCCATGC5050.10525350462906999No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA4990.10400296794040775No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTCTGTTGGGGTGAGTTGATGA4970.10358612237752035No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTTTCCAAGGGCAAATATTTGGTA4940.10296085403318925No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4900.10212716290741444No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4850.10108504900019591No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4810.1002513578744211No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4810.1002513578744211No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)