FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374537
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11220.29956986893150744No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA10950.29236096834224656No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC9210.24590360898923205No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8660.23121881149258955No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8440.22534489249393252No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8430.22507789617581173No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8140.21733500295030933No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8010.21386405081473925No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGAGGGACGTCC7680.20505317231675374No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA7510.2005142349087006No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG7280.19437331959192283No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT7140.190635371138232No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7030.1876984116389035No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC7000.18689742268454118No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6680.17835354050467644No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6380.17034365096105325No Hit
GGTGTGGCATAGCAGCAGGATGAAGAGGAATATGATAAAACGCCGCAGAC6070.16206676509930928No Hit
GGCATAGACCTGACCGTTGCCGAGCAACGGGGTAAAGGTGCAGGTATTGT5780.15432387187380686No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5760.15378987923756532No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAACTTCAT5740.15325588660132378No Hit
GGTCTCTGCCAAGTGTTTGCTGACGCAACCCCCACTGGATGGGGCTTGGC5720.15272189396508223No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5660.15111991605635758No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5620.1500519307838745No Hit
ACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAG5600.14951793814763295No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGCGTATGTAAATAATGCCTAGT5590.14925094182951218No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5370.14337702283085516No Hit
ACTCCGTTGTCCTCTCTCGGAAATACACCTCCTTCCCATGGCTGCTCGGA5310.14177504492213053No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5250.14017306701340587No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT5230.13963907437716433No Hit
CTTATAAGGCCTTTCTGTGTCAACAATACCTGCACCTTTACCCCGTTGCT5050.13483314065099042No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4900.13082819587917882No Hit
CTACCGTCCCCTTCTTCATCTGCCGTTCCAGCCGACCACGGGGCGCACCT4860.12976021060669574No Hit
CAACAGAGTTGTCAGTCCCGATAAGTTTTGCTCCAGACCGGCTGCGAGCA4820.12869222533421265No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATATCCTCTTGT4820.12869222533421265No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4790.1278912363798503No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAATACAATA4770.12735724374360877No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4760.12709024742548802No Hit
GTATAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC4740.12655625478924645No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG4730.12628925847112568No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4660.12442028424428025No Hit
GTTTCAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA4660.12442028424428025No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4650.1241532879261595No Hit
GTATTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT4570.12201731738119331No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC4530.12094933210871023No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG4530.12094933210871023No Hit
CTATTAATAATATCCTCTTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG4500.1201483431543479No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT4440.11854636524562326No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAA4430.1182793689275025No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4350.11614339838253632No Hit
GTAGAGTTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTGCT4340.11587640206441553No Hit
TGTCAGTCCCGATAAGTTTTGCTCCAGACCGGCTGCGAGCAAAACAAGCT4280.1142744241556909No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4240.1132064388832078No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC4230.11293944256508703No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGTCTTGGCCCCCAATACCACAT4170.11133746465636239No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG4160.1110704683382416No Hit
GAACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAAC4160.1110704683382416No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTATT4140.11053647570200006No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4100.10946849042951698No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTATAAGTTGGCGA4070.10866750147515467No Hit
ACCAAAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAG4030.10759951620267155No Hit
GGACCGACAACTCCGTTGTCCTCTCTCGGAAATACACCTCCTTCCCATGG4030.10759951620267155No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4000.10679852724830925No Hit
CCTCTCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3990.10653153093018847No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA3950.10546354565770538No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCGGC3930.10492955302146383No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT3930.10492955302146383No Hit
GCTTTGTGGAATGTTGTGGAGTTCCACTGCATGGCCTGAGGATGACTGTC3920.10466255670334307No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT3910.10439556038522228No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGATGGGGCTTGGC3900.10412856406710153No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG3870.1033275751127392No Hit
GGATATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG3830.1022595898402561No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG3830.1022595898402561No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTATTCCCTTAAC3820.10199259352213534No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA3780.10092460824965223No Hit
ATGTAAGACCTTAGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC3770.10065761193153147No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT3750.10012361929528993No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)