FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401077
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC18570.46300336344392723No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGTTT12400.30916756632766274No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA11660.29071724382101194No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11590.2889719430433558No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9840.24533942360195177No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACCAGGCATTATTTACAT9210.22963171660304627No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8710.21716528247693084No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA8620.21492132433423008No Hit
GGGTAAAGGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8450.21068273673135085No Hit
CCTACTCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA8310.20719213517603852No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT8300.2069428064935162No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA8300.2069428064935162No Hit
ATTAACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8240.20544683439838235No Hit
ATATGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG8230.20519750571586004No Hit
GGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG7880.19647100182757926No Hit
GTTCCGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA7570.1887418126693877No Hit
ATGTAAGACCTTGGGCAACACCTGGTGGGCGTTCACGGTGGTCTCCATGC7510.1872458405742538No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCACCCAAG7440.18550053979659767No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT7350.18325658165389688No Hit
GTGCTGGTAAATAGACCTATTGATTGGAAAGTATGTCAGAGAATTGTGGG7240.18051396614615148No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGT6990.17428074908309377No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6870.17128880489282605No Hit
CCCTTAACTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCACAA6710.16729954597246913No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT6620.16505558782976834No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6610.16480625914724606No Hit
ATATAAGACCTTGGGCAACACCTGGTGGGCGTTCACGGTGGTCTCCATGC6610.16480625914724606No Hit
GCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACGGAGTTGTCGGTT6480.16156498627445603No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6460.16106632890941142No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6420.16006901417932218No Hit
CATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAG6270.15632908394148753No Hit
GTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCAGG6110.1523398250211306No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6100.1520904963386083No Hit
CTTATATAAGACCTTGGGCAACACCTGGTGGGCGTTCACGGTGGTCTCCA6010.1498465381959075No Hit
GTACAAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT6000.1495972095133852No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC5940.14810123741825137No Hit
GTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5890.1468545940056398No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCCGCCGGACCGTGTG5840.14560795059302828No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT5700.14211734903771595No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5670.14136936299014902No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5590.13937473352997057No Hit
CCCCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5550.13837741879988133No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5380.13413883119700207No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC5350.13339084514943514No Hit
GCCTTAATGCCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTT5330.13289218778439052No Hit
ATTTCCGAGAGAGGACAACGGAGTTGTCGGTTCCGATAAGTTTCGCTCCA5290.13189487305430128No Hit
GTTTTAGTACAATATGTTCTTGTGGTAAAGTACCCCAACATCCAATTACA5190.1294015862290782No Hit
CAATATATGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCA5130.12790561413394436No Hit
GTGTAAACAATATATGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4940.12316836916602049No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGGCAGATGAAGAAGG4920.12266971180097588No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4860.12117373970584203No Hit
TATATAAGACCTTGGGCAACACCTGGTGGGCGTTCACGGTGGTCTCCATG4800.11967776761070818No Hit
GTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCCGG4790.11942843892818586No Hit
GGCCAATAGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGG4760.11868045288061892No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAAGGAGTTTG4740.11818179551557431No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4730.117932466833052No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG4720.11768313815052969No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4690.11693515210296278No Hit
GCACACCCGAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAAC4670.11643649473791816No Hit
GTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGG4650.11593783737287354No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG4650.11593783737287354No Hit
GATTAAAATTAATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT4610.11494052264278432No Hit
TCCTAGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGT4600.11469119396026199No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4550.11344455054765046No Hit
GGATGGGGCTTGGCTATTGGCCATCGCCGCATGCGTGGAACCTTTGTGGC4530.11294589318260584No Hit
GTCCGGCGGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4500.11219790713503891No Hit
CGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTACGTCC4470.11144992108747197No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCGGATGAGAAGGCACAG4430.11045260635738274No Hit
CACACAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCGG4390.1094552916272935No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA4390.1094552916272935No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4380.10920596294477121No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA4350.10845797689720427No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACG4340.10820864821468197No Hit
CTCCCCGTCTGTGCCTTCTCATCCGCCGGACCGTGTGCACTTCGCTTCAC4330.10795931953215966No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAGAGAATTGTGGGTCTTTTGG4280.10671267611954813No Hit
GTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTA4280.10671267611954813No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG4230.10546603270693658No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT4230.10546603270693658No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4190.10446871797684734No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAAC4190.10446871797684734No Hit
CTACTGTACCTGTCTTTAACCCTGAGTGGCAAACTCCTTCCTTTCCTCAC4150.1034714032467581No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCT4120.10272341719919117No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG4110.10247408851666888No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACAT4090.10197543115162426No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4090.10197543115162426No Hit
ATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATACAATCTAAGC4080.10172610246910194No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4070.10147677378657964No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4040.10072878773901271No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)