FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401077
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50051.247890056024155No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC17360.4328345928587279No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGTTT11790.29395851669380196No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA11230.27999611047255263No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11040.2752588655046288No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9200.22938238792052398No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA8690.21666662511188625No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT8610.21467199565170778No Hit
GGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG8120.20245489020811464No Hit
GGGTAAAGGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8050.20070958943045847No Hit
ATATGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG8030.20021093206541388No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTGGT7930.19771764524019078No Hit
ATCCTGAACATGCAGTTAATCATTACTTCAAAACCAGGCATTATTTACAT7810.1947257010499231No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCACCCAAG7550.18824315530434307No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7380.1840045677014638No Hit
GTGCTGGTAAATAGACCTATTGATTGGAAAGTATGTCAGAGAATTGTGGG7330.18275792428885226No Hit
GTTCCGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA7330.18275792428885226No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG7090.17677403590831686No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCCGCCGGACCGTGTG6970.17378209171804915No Hit
CCCTTAACTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCACAA6960.17353276303552684No Hit
ATGTAAGACCTTGGGCAACACCTGGTGGGCGTTCACGGTGGTCTCCATGC6820.17004216148021453No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT6530.16281162968706758No Hit
ATTAACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT6490.16181431495697832No Hit
GTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCAGG6460.16106632890941142No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG6340.1580743847191437No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6190.15433445448130909No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCATCTGCCGTTCCGGCCGACCACG6170.15383579711626447No Hit
GTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6100.1520904963386083No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT5960.14859989478329597No Hit
GTACAAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT5760.1436133211328498No Hit
ATATAAGACCTTGGGCAACACCTGGTGGGCGTTCACGGTGGTCTCCATGC5680.14161869167267133No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT5620.14012271957753747No Hit
CCCCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5530.1378787614348367No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5520.1376294327523144No Hit
CCTACTCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA5490.13688144670474744No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGGCGATGGCC5450.13588413197465823No Hit
GCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACGGAGTTGTCGGTT5440.13563480329213592No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5430.13538547460961362No Hit
GTTTTAGTACAATATGTTCTTGTGGTAAAGTACCCCAACATCCAATTACA5400.1346374885620467No Hit
GTGTAAACAATATATGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC5350.13339084514943514No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5280.131645544371779No Hit
CATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAG5280.131645544371779No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5250.13089755832421207No Hit
GCCTTAATGCCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTT5240.13064822964168973No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA5190.1294015862290782No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5130.12790561413394436No Hit
GTATGTAAATAATGCCTGGTTTTGAAGTAATGATTAACTGCATGTTCCGG5130.12790561413394436No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC5010.12491366994367664No Hit
CAATATATGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCA4980.12416568389610973No Hit
ATTTCCGAGAGAGGACAACGGAGTTGTCGGTTCCGATAAGTTTCGCTCCA4830.1204257536582751No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4810.11992709629323048No Hit
CGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTACGTCC4770.11892978156314124No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT4770.11892978156314124No Hit
CTCCCCGTCTGTGCCTTCTCATCCGCCGGACCGTGTGCACTTCGCTTCAC4760.11868045288061892No Hit
CTTATATAAGACCTTGGGCAACACCTGGTGGGCGTTCACGGTGGTCTCCA4750.11843112419809662No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT4670.11643649473791816No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4640.11568850869035123No Hit
GTTCAGGATAATATGGTTTAATGCCTTTGTCCAAGGGCAAATATTTGGTA4640.11568850869035123No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4610.11494052264278432No Hit
GGATGGGGCTTGGCTATTGGCCATCGCCGCATGCGTGGAACCTTTGTGGC4610.11494052264278432No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAAGGAGTTTG4570.11394320791269508No Hit
GTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGG4450.11095126372242736No Hit
ATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATACAATCTAAGC4400.10970462030981583No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4360.10870730557972659No Hit
CATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4310.10746066216711504No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG4290.10696200480207042No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4270.1064633474370258No Hit
GATTAAAATTAATTATGCCTGCTAGGTTCTATCCTAACCTTACCAAATAT4260.1062140187545035No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4240.10571536138945888No Hit
GATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTG4150.1034714032467581No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA4110.10247408851666888No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCGGATGAGAAGGCACAG4080.10172610246910194No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4030.10047945905649042No Hit
GGCCAATAGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGG4020.1002301303739681No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)