FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459816
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA17090.3716704072933521No Hit
CCTCCCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13760.29925013483654334No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11360.2470553438766811No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCTTTGACATACT10450.22726481897106668No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10170.2211754266924161No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9690.21073646850044364No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTACTTCCGAGAGAGGACAACAGAGT9530.20725681576978616No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG9470.20595194599578961No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG9410.20464707622179307No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT9340.2031247281521304No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC8820.1918158567774936No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8690.1889886389338344No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA8410.1828992466551838No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA8130.17680985437653324No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8000.17398263653287402No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7820.17006802721088435No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7670.16680585277589297No Hit
GCACGGGACCATGCAAGACCTGCACAATTCCTGCTCAAGGAACCTCTATG7650.16637089618456077No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC7260.15788924265358317No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7220.1570193294709188No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7160.15571445969692224No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7080.1539746333315935No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7010.15245228526193086No Hit
ATATAGAGCTGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAAGAAGT6970.15158237207926648No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA6850.14897263253127338No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6770.14723280616594464No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6700.14571045809628197No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6660.1448405449136176No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6650.14462306661795155No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6640.14440558832228542No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6450.14027350070462968No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC6430.1398385441132975No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6350.13809871774796875No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6300.1370113262696383No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6270.13635889138264No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6260.13614141308697392No Hit
CTCTAAGGCCTCCCGATATAGAGCTGAGGCGGTGTCGAGGAGATCTCGAA6160.133966630130313No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT6060.13179184717365205No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6050.131574368877986No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6010.1307044556953216No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5980.13005202080832332No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5970.12983454251265725No Hit
CTATCGTCCCCTCCTTCGTCTGCCGTTCCGGCCGACCACGGGGCGCACCT5930.12896462932999286No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5930.12896462932999286No Hit
GCTTTGGGGCATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTGG5770.12548497659933538No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5750.1250500200080032No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGGCAGACGAAGGAGG5740.1248325417123371No Hit
TGCAAGACCTGCACAATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5680.12352767193834055No Hit
CCTATAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTT5530.12026549750334917No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5490.11939558432068478No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5420.11787323625102215No Hit
GGAACATATTGTACTAAAACTTAAGCAATGTTTTCGAAAACTGCCTGTAA5380.11700332306835777No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTAAGTTTTAGTACAATA5380.11700332306835777No Hit
CCTAGTACAAAGACCTTTAGCCTAATCTCCTCCCCCAACTCCTCCCAGTC5370.11678584477269169No Hit
GGTTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTG5350.1163508881813595No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5340.11613340988569341No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG5290.11504601840736295No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5230.1137411486333664No Hit
GTACTAAAACTTAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT5220.11352367033770029No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT5210.11330619204203421No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5200.11308871374636811No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5180.11265375715503592No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5140.11178384397237157No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA5130.11156636567670546No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTACTAATAATATCCTCCTGT5060.11004401760704283No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCT5030.10939158272004455No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA4860.10569445169372096No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4820.1048245385110566No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4790.10417210362405832No Hit
GGATGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC4790.10417210362405832No Hit
ACATAGAGGTTCCTTGAGCAGGAATTGTGCAGGTCTTGCATGGTCCCGTG4770.10373714703272613No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4710.10243227725872958No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA4710.10243227725872958No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4690.10199732066739739No Hit
CTTTGGGGCATGGACATTGACCCATATAAAGAATTTGGAGCTTCTGTGGA4690.10199732066739739No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC4630.10069245089340084No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4620.10047497259773476No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)