FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339883
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC15210.44750693621040183No Hit
CCTCCTGCATATAGGCCACCAAATGCCCCTATCTTATCAACACTTCCGGA14530.4275000514883063No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA14130.4157312957694266No Hit
CTGTAAGAGGCCCCACAAATTGCTCACATCTATCAACAATGTCCTCCTGC13100.38542674979331126No Hit
ACTTACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT12180.35835861163988786No Hit
GTTGTAAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCGTG11980.35247423378044795No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC10550.31040093208545294No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGAAACACCCGTG10010.2945131118649653No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG9500.27950794832339365No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC9120.2683276303904579No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA8190.2409652733440625No Hit
CTGTTAGAGGCCCCACAAATTGCTCACATCTATCAACAATGTCCTCCTGC8030.2362577710565106No Hit
TTTTACGGGTCACCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTT7880.23184448766193072No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC7630.22448901533763088No Hit
CTCTTACAGTAAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGG7470.219781513050079No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7430.21860463747819103No Hit
GATAAGGGAGTTGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGAGG7430.21860463747819103No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTACGGGTCACCATATT7260.21360291629766714No Hit
CTCCCCTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGG6960.20477634950850734No Hit
GTATTCTGAGGATTTTTGTCAACGAGAAAAACCCCGCCTGTAACACGAGC6540.19241915600368362No Hit
CTTTAGGACATGGACATTGACGTGTATAAAGAATTTGGAGCTTCTGTGGA6540.19241915600368362No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT6490.19094806153882365No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA6440.1894769670739637No Hit
CCTTCTATTCGAGATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGC6300.18535790257235576No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT6240.18359258921452382No Hit
GGACTAACCCTGGCCCGAATGCTCCCACTCCCACCTTGTTGGCGTCCGGC6050.17800243024805595No Hit
CTCTAACAGTAAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGG5890.17329492796050405No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5860.1724122712815881No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATGACATATCCCATGAA5860.1724122712815881No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG5860.1724122712815881No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA5790.17035273903078413No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5780.17005852013781214No Hit
CCTCCCGCATATAGGCCACCAAATGCCCCTATCTTATCAACACTTCCGGA5560.16358570449242826No Hit
ATTTTGAGCAGGGCTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5360.15770132663298841No Hit
ATTCTGAGCAGGGCTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5300.15593601327515647No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5260.1547591377032685No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG5250.15446491881029648No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5070.1491689787368006No Hit
GGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGGCCCT4940.1453441331281647No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC4930.1450499142351927No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT4900.1441672575562767No Hit
GGTCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCC4860.14299038198438874No Hit
GGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTA4820.14181350641250076No Hit
GTATTCTGAGCAGGGCTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAA4770.1403424119476408No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT4740.1394597552687248No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGCACGACAGAAT4730.13916553637575282No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4720.13887131748278084No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4680.13769444191089286No Hit
ATCGACGACTGGGGACCCTGTACCGAACATGGAGAACATCGCATCAGGAC4640.1365175663390049No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG4640.1365175663390049No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4590.13504647187414492No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAAAGGA4570.13445803408820095No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA4540.13357537740928496No Hit
CTCACAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCT4520.132986939623341No Hit
CCCCAACAGTCCCCCGTGGGGCGGGACTAACCCTGGCCCGAATGCTCCCA4520.132986939623341No Hit
GTATAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG4480.131810064051453No Hit
GTTTACAGGGAGTTTCCTAAAACACATTCTGATTTTTTGTACAATATGTT4410.12975053180064905No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTTTGCATGGTCCGGTG4360.1282794373357891No Hit
GCATATAGGCCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTAC4320.1271025617639011No Hit
GGATAATAAGGTTTGATTCCTTTATCTAAGGGCAAATATTTAGTGACATT4310.1268083428709291No Hit
ATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA4290.12621990508498512No Hit
GGGTTAGTCCCGCCCCACGGGGGACTGTTGGGGTGGAGCCCTCAGGCTCA4260.12533724840606913No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT4250.12504302951309715No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4220.12416037283418117No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4210.12386615394120919No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGGCCACCAAATGCC4190.12327771615526519No Hit
CCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTTGGTCTTCCAAAC4160.12239505947634921No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4140.12180662169040524No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT4080.12004130833257326No Hit
CAGTAAGCCCTGCTCAAAATACTGTCTCAGCCACATCGTCAATCTTATCG4060.11945287054662929No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC3990.11739333829582532No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT3960.11651068161690935No Hit
CATCCAGCGATAGCCAGGACAAATTGGAGGACAACAGGTTGGTGAGTGAC3950.11621646272393735No Hit
ATACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAG3900.1147453682590774No Hit
ATTGCACACCTCACCATACGGCACTCAGGCAAGCTATTCTGTGTTGGGGT3890.11445114936610541No Hit
CCCCTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA3850.11327427379421741No Hit
ATGCAGGAGGAGTGCGAATCCACACTCCAAAAGATACCAAATATTCAAGA3840.11298005490124542No Hit
GTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCCGCCTGTAACACG3820.11239161711530145No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA3780.11121474154341347No Hit
CCCATAAAATGAGGCGCTACGTGTGGACTCTCTTTTATATAAGATACCCG3770.11092052265044147No Hit
ATCCATCATCCAGGGAAGCAGTAGTCGGCTATGTCAACGTTAATATGGGC3750.11033208486449748No Hit
AAGTCAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCTGGTC3710.10915520929260952No Hit
GTTCTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCCGT3670.10797833372072155No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT3570.1050361447910016No Hit
GATGAAAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTG3570.1050361447910016No Hit
GGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGGCCCTG3500.10297661254019766No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG3490.10268239364722566No Hit
CAATAGGCCTGTTTACAGGGAGTTTCCTAAAACACATTCTGATTTTTTGT3480.10238817475425367No Hit
ATCCCGATTGGGACCTCAACCCGCACAAGGACAACTGGCCGGACGCCAAC3480.10238817475425367No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGCAGGAGTTT3440.1012112991823657No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA3440.1012112991823657No Hit
GGAACATATTGTACAAAAAATCAGAATGTGTTTTAGGAAACTCCCTGTAA3430.10091708028939371No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)