FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541788
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC32510.6000502041388882No Hit
ATATAAGAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT26080.4813690963993296No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG25090.46309626643631824No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA21780.40200225918625No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC20470.3778230599422653No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT18750.3460763250570334No Hit
CTCTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGAGT18190.33574017881532997No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG17640.325588606613657No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC17230.3180210709724099No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG15350.2833211514466913No Hit
CCTCCTGCATATAGACCATCAAATGCCCCTATCTTATCAACGCTTCCGGA15130.27926052256602213No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG12890.23791593759920857No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG12310.22721064327744434No Hit
ATATGTTCCTGTGGCAATGTTCCCCAACTCCCAATTACATATCCCATGAA12190.2249957547970793No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA11960.22075055187637968No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGG11660.21521333067546716No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG11380.21004525755461545No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT11090.20469261039373332No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA10810.19952453727288166No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAGAGAGAGTCCACACGTAGCGCC10680.19712507475248622No Hit
CTTTGGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA10680.19712507475248622No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT10480.19343359395187787No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG10300.19011126123133032No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT10240.18900381699114782No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC10110.1866043544707524No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA10060.1856814842706003No Hit
TCCAAAGAGTGTGTAAATAATGTCGAGTCTGGAAGTAATGATTAACTACA10010.18475861407044822No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9990.18438946599038739No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC9980.18420489195035697No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT9910.18291287367014405No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9750.17995968902965737No Hit
GCCTAAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTG9710.1792213928695357No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC9590.17700650438917068No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA9590.17700650438917068No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACTCGACATTATT9450.17442246782874482No Hit
CTCCAAAAGACACCAAATATTCAAGAACAATTTCTCTCCCAAAAGTAAGA9380.1731304495485319No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG9340.17239215338841024No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT9100.16796237642768022No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA9010.16630121006740642No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG8910.16445546966710226No Hit
GCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT8830.16297887734685892No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC8510.15707250806588555No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG8480.1565187859457943No Hit
GTATGTAGTTAATCATTACTTCCAGACTCGACATTATTTACACACTCTTT8460.15614963786573346No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT8420.1554113417056118No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTCGGAAACTTCCTGTAA8380.1546730455454901No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG8270.15264273110515553No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG8140.1502432685847601No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA8130.15005869454472967No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA8080.1491358243445776No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC8000.14765923202433423No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA7920.1461826397040909No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA7770.14341402910363463No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC7680.14175286274336088No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT7650.1411991406232696No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG7600.14027627042311752No Hit
TATCCACATTGCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG7580.1399071223430567No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC7500.13843053002281336No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7480.13806138194275253No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT7450.13750765982266125No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT7420.13695393770257No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT7390.13640021558247875No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACT7340.13547734538232667No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC7330.13529277134229625No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC7320.13510819730226584No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCATTGTC7220.13326245690196165No Hit
GTTTACAGGAAGTTTCCGAAAACACATTTTGATTTTTTGTACAATATGTT7190.1327087347818704No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCATCAAATGCC7140.13178586458171831No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7140.13178586458171831No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA7060.13030927226147498No Hit
ATTGTAAGGGGCCCCACAAATTGCCTACATCTATCAACAATGTCCTCCTG7050.13012469822144454No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG7040.12994012418141412No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC7040.12994012418141412No Hit
GGATATCCACATTGCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAAT7020.1295709761013533No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGGCACTCAGGCA7020.1295709761013533No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACTCGACATTATTTACAC7010.12938640206132287No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG6980.12883267994123163No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCGAAAACACATTTTGATTTTTTGT6970.1286481059012012No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6900.1273560876209883No Hit
GTACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGTGGTGG6880.12698693954092743No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG6760.12477205106056243No Hit
GCATATAGACCATCAAATGCCCCTATCTTATCAACGCTTCCGGAAACTAC6670.12311088470028868No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA6570.1212651442999845No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGAAC6530.12052684813986282No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA6460.11923482985964991No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA6430.11868110773955866No Hit
CCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTTGGTCTTCCAAAC6350.11720451541931531No Hit
TTGTACACCTCACCATACGGCACTCAGGCAAGCTATTCTGTGTTGGGGTG6290.1160970711791328No Hit
GATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGGGGA6290.1160970711791328No Hit
CCCCTATCTTATCAACGCTTCCGGAAACTACTGTTGTTAGACAACGGGGC6280.11591249713910237No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6220.11480505289891986No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA6190.11425133077882861No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTGACTT6170.11388218269876779No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6120.1129593124986157No Hit
GTACCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCCGA6080.11222101633849402No Hit
ATGTAATTGGGAGTTGGGGAACATTGCCACAGGAACATATTGTACAAAAA6020.1111135720983115No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGAACATTGCCACAG6020.1111135720983115No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5940.10963697977806819No Hit
CCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCACTCACC5910.10908325765797693No Hit
GTCTAACAACAGTAGTTTCCGGAAGCGTTGATAAGATAGGGGCATTTGAT5890.1087141095779161No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA5800.10705294321764233No Hit
CCTTATGTGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5770.10649922109755108No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5760.10631464705752064No Hit
CGTTAATATGGGCCTAAAAATCAGACAATTATTGTGGTTTCACATTTCCT5760.10631464705752064No Hit
GTATTCGGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAG5650.10428433261718607No Hit
GCCTACATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA5650.10428433261718607No Hit
CATATAGACCATCAAATGCCCCTATCTTATCAACGCTTCCGGAAACTACT5640.10409975857715564No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5600.10336146241703398No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG5590.10317688837700355No Hit
CCCCATGGGGGACTGTTGGGGTGGAGCCCTCAGGCTCAGGGCCTACTCAC5560.10262316625691228No Hit
ACCCTGTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTG5550.10243859221688188No Hit
GTTCCTGAACTGGAGCCACCAGCAGGAAGGTACAGGGCCCTGACTCTGGG5530.10206944413682104No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC5480.10114657393666897No Hit
CCCCAACACAGAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGTACAATG5480.10114657393666897No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGTC5460.10077742585660812No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG5450.1005928518165777No Hit
TAGCAGCAGGATGCAGAGGAAGATGATAAAACGCCGCAGACACATCCAGC5430.10022370373651687No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT5420.10003912969648646No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)