FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541788
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC29160.5382179007286984No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG25370.46826433955716995No Hit
ATATAAGAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT24640.45479043463494945No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC18820.3473683433372463No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT18280.3374013451756037No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA17740.3274343470139612No Hit
CTCTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGAGT17470.3224508479331399No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG16650.30731577665064563No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC15660.28904294668763425No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG15530.28664348416723884No Hit
CCTCCTGCATATAGACCATCAAATGCCCCTATCTTATCAACGCTTCCGGA14880.2746461715652617No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA13130.24234571455993859No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG12590.232378716398296No Hit
ATATGTTCCTGTGGCAATGTTCCCCAACTCCCAATTACATATCCCATGAA11640.2148441825954063No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGG11490.21207557199495003No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT11140.20561548059388543No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGACATG10970.20247772191336832No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC10730.1980479449526383No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAGAGAGAGTCCACACGTAGCGCC10690.1973096487925166No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT10550.19472561223209078No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT10500.19380274203193867No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10500.19380274203193867No Hit
CTCCAAAAGACACCAAATATTCAAGAACAATTTCTCTCCCAAAAGTAAGA10420.19232614971169534No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT10160.1875272246709045No Hit
CTTTGGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA9770.1803288371097182No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC9650.17811394862935317No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9580.17682193034914026No Hit
TCCAAAGAGTGTGTAAATAATGTCGAGTCTGGAAGTAATGATTAACTACA9520.17571448610895773No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG9260.17091556106816688No Hit
GCCTAAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTG9230.17036183894807563No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA9200.16980811682798438No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA9110.1681469504677106No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA9050.1670395062275281No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC8860.16353259946695017No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA8850.16334802542691976No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA8620.15910282250622015No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACTCGACATTATT8580.15836452634609846No Hit
GTATGTAGTTAATCATTACTTCCAGACTCGACATTATTTACACACTCTTT8310.1533810272652772No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT8240.15208900898506428No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT8220.15171986090500345No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG8210.15153528686497303No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG8090.149320398384608No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT8070.14895125030454717No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA8050.14858210222448634No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA7980.14729008394427343No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7940.14655178778415173No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG7920.1461826397040909No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC7900.14581349162403007No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7740.14286030698354338No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG7680.14175286274336088No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG7670.14156828870333046No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTCGGAAACTTCCTGTAA7570.13972254830302627No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC7530.1389842521429046No Hit
GCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT7380.13621564154244833No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC7380.13621564154244833No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG7310.13492362326223542No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC7210.13307788286193123No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCATCAAATGCC7190.1327087347818704No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA7180.13252416074183998No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG7170.13233958670180956No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA7100.13104756842159665No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT7060.13030927226147498No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7020.1295709761013533No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6980.12883267994123163No Hit
TATCCACATTGCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG6970.1286481059012012No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6960.1284635318611708No Hit
GTTTACAGGAAGTTTCCGAAAACACATTTTGATTTTTTGTACAATATGTT6960.1284635318611708No Hit
GTACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGTGGTGG6940.12809438378110993No Hit
GTACCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCCGA6810.12569492126071452No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC6800.1255103472206841No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG6800.1255103472206841No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACTCGACATTATTTACAC6750.12458747702053202No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACT6750.12458747702053202No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6630.12237258854016701No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6610.12200344046010617No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG6600.12181886642007576No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA6570.1212651442999845No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGGCACTCAGGCA6550.12089599621992367No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCATTGTC6490.11978855197974116No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA6400.1181273856194674No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCGAAAACACATTTTGATTTTTTGT6380.11775823753940656No Hit
GCATATAGACCATCAAATGCCCCTATCTTATCAACGCTTCCGGAAACTAC6370.11757366349937615No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT6340.1170199413792849No Hit
ATTGTAAGGGGCCCCACAAATTGCCTACATCTATCAACAATGTCCTCCTG6330.11683536733925447No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6300.11628164521916322No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGAACATTGCCACAG6240.1151742009789807No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA6230.11498962693895029No Hit
GGATATCCACATTGCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAAT6220.11480505289891986No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6210.11462047885888946No Hit
CCCCTATCTTATCAACGCTTCCGGAAACTACTGTTGTTAGACAACGGGGC6130.11314388653864611No Hit
TTGTACACCTCACCATACGGCACTCAGGCAAGCTATTCTGTGTTGGGGTG6100.11259016441855485No Hit
CCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCACTCACC6090.11240559037852445No Hit
GTCTAACAACAGTAGTTTCCGGAAGCGTTGATAAGATAGGGGCATTTGAT6090.11240559037852445No Hit
CCCCAACACAGAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGTACAATG5960.11000612785812901No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5950.10982155381809859No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA5950.10982155381809859No Hit
CCTTATGTGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5770.10649922109755108No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5760.10631464705752064No Hit
GTATTCGGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAG5750.10613007301749024No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGTC5730.10576092493742939No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5720.10557635089739899No Hit
GCCTACATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA5700.10520720281733814No Hit
ATGTAATTGGGAGTTGGGGAACATTGCCACAGGAACATATTGTACAAAAA5610.10354603645706438No Hit
GATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGGGGA5600.10336146241703398No Hit
GTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT5570.10280774029694272No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG5560.10262316625691228No Hit
AGATAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGAG5490.10133114797669937No Hit
CATATAGACCATCAAATGCCCCTATCTTATCAACGCTTCCGGAAACTACT5450.1005928518165777No Hit
ATATAACTGAAAGCCAGACAATGGGGGAAAGCCCTACGAACCACTGAACA5450.1005928518165777No Hit
CGTTAATATGGGCCTAAAAATCAGACAATTATTGTGGTTTCACATTTCCT5440.10040827777654728No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA5420.10003912969648646No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)