FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251295
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11610.4620068047513878No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10240.4074892059133688No Hit
TCTGCTGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTACTATTCG9650.38401082393203206No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8870.3529716070753497No Hit
GTGCTGGTGGACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8800.3501860363318013No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8100.3223303288963171No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG7400.29447462146083286No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGGGGACAAACGGGCAAC7060.28094470642074054No Hit
CCATAATAGTTGTCTGATTTTTATGCCCATATTAACGTTGACATAGCTGA6660.2650271593147496No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6300.2507013669193577No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTACTATTCG6110.24314053204401204No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5970.23756939055691517No Hit
TAAATGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATCATC5940.23637557452396585No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT5840.23239618774746812No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG5760.22921267832626993No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA5490.218468334029726No Hit
GAATATTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5450.2168765793191269No Hit
CTCTTTGGAAGGCGGGAATCTTATATAAAAGAGAGTCCACACGTAGCGCC5330.2121013151873296No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATACATTGGAA5170.20573429634493326No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5040.20056109353548618No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5020.19976521618018667No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT4980.19817346146958756No Hit
CTATTATGGTTTCACATTTCCTGTCTCACTTTTGGAAGAGAAACTGTTCT4940.19658170675898845No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT4720.1878270558506934No Hit
GTTAATATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC4610.18344973039654588No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG4550.18106209833064724No Hit
CTTTTATATAAGATTCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT4380.17429714081060108No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA4370.17389920213295132No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA4290.17071569271175313No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4290.17071569271175313No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA4220.1679301219682047No Hit
CTGTAAGGGGCCCCACAAATTGCTCACATCTATCAACAATGTCCTCCTGC4210.16753218329055491No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT4200.16713424461290516No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4190.16673630593525537No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4170.16594042857995583No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA4150.16514455122465627No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACGG4100.1631548578364074No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4040.16076722577050875No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAGGT4030.160369287092859No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATACATTGGAACTCCACCACGTT4020.1599713484152092No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT3990.1587775323822599No Hit
ATCAAGGTATGTTGCCCGTTTGTCCCCTAATTCCAGGATCATCAACGACC3870.1540022682504626No Hit
ACTCTTATGGTTTCACATTTCCTGTCTCACTTTTGGAAGAGAAACTGTTC3810.15161463618456394No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG3750.14922700411866532No Hit
GGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA3730.14843112676336578No Hit
GTCTTCGATAAGATTGACGATATGGCAGAGACAGTATTCTGAGCAGGGCT3720.148033188085716No Hit
GTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATT3660.14564555601981735No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA3660.14564555601981735No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTGAGA3660.14564555601981735No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC3640.14484967866451778No Hit
ACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACC3610.1436558626315685No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCCTATAGACCACCAAATGCC3590.14285998527626892No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG3580.14246204659861916No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC3550.14126823056566984No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG3510.13967647585507073No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG3480.1384826598221214No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT3420.13609502775622276No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTCT3410.135697089078573No Hit
ACATTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCACTCCC3370.1341053343679739No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT3360.1337073956903241No Hit
CCCTTACAGTAAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGG3330.1325135796573748No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT3310.13171770230207525No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC3290.1309218249467757No Hit
GTATGGATCGGCAGAGGAGACCCAAAGGTTCCACGCATGCGCTGATGGCC3270.13012594759147617No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG3260.12972800891382638No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC3230.12853419288087706No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTCGGAAACTTCCTGTAA3220.1281362542032273No Hit
CATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGT3210.1277383155255775No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA3170.1261465608149784No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA3160.12574862213732863No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC3110.12375892874907977No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG3010.11977954197258203No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG2990.11898366461728248No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTGAGACA2940.11699397122903361No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCTACCTGGGTGGGAAGTAATTTGG2940.11699397122903361No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA2920.11619809387373406No Hit
ATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCT2910.11580015519608428No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT2910.11580015519608428No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG2880.11460633916313497No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT2870.11420840048548518No Hit
GTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT2840.11301458445253587No Hit
CTGCTGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTACTATTCGA2830.11261664577488607No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA2820.11221870709723632No Hit
TCCTCAGGCCATACATTGGAACTCCACCACGTTCCACCAAACTCTTCAAG2820.11221870709723632No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT2820.11221870709723632No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT2810.11182076841958655No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT2800.11142282974193676No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA2780.11062695238663722No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG2770.11022901370898745No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG2760.10983107503133767No Hit
GAACAGTGAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCAATCTTA2740.10903519767603811No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA2730.10863725899838836No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA2730.10863725899838836No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG2720.10823932032073856No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTACTATTCG2720.10823932032073856No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA2700.10744344296543902No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGTGTCCAAGGAATATTAAC2680.10664756561013947No Hit
CCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTTGGACTTCCAAAC2670.10624962693248971No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA2660.10585168825483994No Hit
GCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACGGCACTCAGGCA2600.10346405618894129No Hit
TATTAACGTTGACATAGCTGACTACCAATTCCCTGGATCCTGGATCTTCC2600.10346405618894129No Hit
GGCCATACATTGGAACTCCACCACGTTCCACCAAACTCTTCAAGATCCCA2590.10306611751129151No Hit
ACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGG2570.10227024015599195No Hit
GGGTTCACCCCTCCCCATGGGGGACTGTTGGGGTGGAGCCCTCAGGCTCA2560.1018723014783422No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCA2560.1018723014783422No Hit
CATTGGAACTCCACCACGTTCCACCAAACTCTTCAAGATCCCAGAGTCAG2560.1018723014783422No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG2550.1014743628006924No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA2550.1014743628006924No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG2540.10107642412304264No Hit
CCATACGGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAATC2530.10067848544539286No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT2530.10067848544539286No Hit
GTCCAAGGAATATTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC2520.10028054676774309No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)