FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251295
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10610.4222129369864104No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA8910.35456336178594877No Hit
TCTGCTGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTACTATTCG8020.3191468194751189No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT7950.31636124873157045No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG7600.3024333950138284No Hit
GTGCTGGTGGACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7300.29049523468433514No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGGGGACAAACGGGCAAC7050.2805467677430908No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6890.2741797489006944No Hit
CCATAATAGTTGTCTGATTTTTATGCCCATATTAACGTTGACATAGCTGA6370.25348693766290614No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5760.22921267832626993No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTACTATTCG5680.22602916890507174No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA5660.22523329154977215No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG5560.22125390477327445No Hit
CTCTTTGGAAGGCGGGAATCTTATATAAAAGAGAGTCCACACGTAGCGCC5490.218468334029726No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT5470.2176724566744265No Hit
GAATATTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5430.2160807019638274No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT5390.21448894725322828No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATACATTGGAA5300.2109074991543803No Hit
CTATTATGGTTTCACATTTCCTGTCTCACTTTTGGAAGAGAAACTGTTCT5100.2029487256013848No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5010.19936727750253685No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG4810.19140850394954137No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT4780.19021468791659205No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT4720.1878270558506934No Hit
TAAATGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATCATC4540.18066415965299748No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA4470.17787858890944905No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACGG4310.17151157006705267No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4170.16594042857995583No Hit
CTGTAAGGGGCCCCACAAATTGCTCACATCTATCAACAATGTCCTCCTGC4120.16395073519170697No Hit
CTTTTATATAAGATTCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT4100.1631548578364074No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4090.16275691915875762No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA4070.1619610418034581No Hit
GGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4030.160369287092859No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG3980.1583795937046101No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAGGT3980.1583795937046101No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA3960.15758371634931057No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATACATTGGAACTCCACCACGTT3960.15758371634931057No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT3870.1540022682504626No Hit
GTTAATATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC3840.1528084522175133No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTGAGA3750.14922700411866532No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA3730.14843112676336578No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC3720.148033188085716No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG3710.14763524940806622No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC3710.14763524940806622No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCCTATAGACCACCAAATGCC3680.1464414333751169No Hit
ACTCTTATGGTTTCACATTTCCTGTCTCACTTTTGGAAGAGAAACTGTTC3560.14166616924331962No Hit
GTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATT3530.14047235321037027No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG3480.1384826598221214No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3460.13768678246682187No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT3400.13529915040092322No Hit
ACATTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCACTCCC3370.1341053343679739No Hit
ATCAAGGTATGTTGCCCGTTTGTCCCCTAATTCCAGGATCATCAACGACC3360.1337073956903241No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTCGGAAACTTCCTGTAA3350.13330945701267435No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC3310.13171770230207525No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC3180.1265444994926282No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC3180.1265444994926282No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG3170.1261465608149784No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG3170.1261465608149784No Hit
ACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACC3160.12574862213732863No Hit
CCCTTACAGTAAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGG3160.12574862213732863No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCA3150.12535068345967884No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA3150.12535068345967884No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT3140.12495274478202908No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA3110.12375892874907977No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC3100.12336099007142999No Hit
GTCTTCGATAAGATTGACGATATGGCAGAGACAGTATTCTGAGCAGGGCT3080.12256511271613044No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG3070.12216717403848068No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT3040.12097335800553134No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG3040.12097335800553134No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTCT3020.12017748065023179No Hit
CATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGT2950.11739190990668338No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT2890.11500427784078474No Hit
ATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCT2850.11341252313018563No Hit
CCATACGGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAATC2770.11022901370898745No Hit
GCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACGGCACTCAGGCA2760.10983107503133767No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG2750.1094331363536879No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG2740.10903519767603811No Hit
GAACAGTGAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCAATCTTA2720.10823932032073856No Hit
GTATGGATCGGCAGAGGAGACCCAAAGGTTCCACGCATGCGCTGATGGCC2710.1078413816430888No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG2700.10744344296543902No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT2700.10744344296543902No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA2700.10744344296543902No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA2690.10704550428778925No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA2680.10664756561013947No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT2670.10624962693248971No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT2660.10585168825483994No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG2660.10585168825483994No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG2660.10585168825483994No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTGAGACA2660.10585168825483994No Hit
GTTTACAGGAAGTTTCCGAAAACACATTTTGATTTTTTGTACAATATGTT2650.10545374957719016No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCTACCTGGGTGGGAAGTAATTTGG2630.1046578722218906No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA2620.10425993354424085No Hit
GTACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAACGTGGTGG2610.10386199486659105No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA2610.10386199486659105No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTACTATTCG2610.10386199486659105No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGGGTC2600.10346405618894129No Hit
AGATAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGAG2600.10346405618894129No Hit
TCCTCAGGCCATACATTGGAACTCCACCACGTTCCACCAAACTCTTCAAG2590.10306611751129151No Hit
CATTGGAACTCCACCACGTTCCACCAAACTCTTCAAGATCCCAGAGTCAG2580.10266817883364174No Hit
CTGCTGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTACTATTCGA2560.1018723014783422No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG2560.1018723014783422No Hit
AGATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCG2550.1014743628006924No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA2530.10067848544539286No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCCCTAATTCCAGGATC2530.10067848544539286No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA2530.10067848544539286No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)