FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349792
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC21140.6043591620162839No Hit
GTCTCTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT18140.5185939072363004No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG15450.4416910621169152No Hit
GTACCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA14950.42739685298691793No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA13570.3879448357881255No Hit
CCCAGAGACAACAGAAAATTGGTAACAGCGGCATAAAGGGACTCAAGATG13270.3793683103101272No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11740.3356280303723356No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11720.3350562620071357No Hit
CCCAAAGACAACAGAAAATTGGTAACAGCGGCATAAAGGGACTCAAGATG11250.32161970542493823No Hit
ATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA10850.31018433812094043No Hit
CCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTGCTGGTTGTTGA10410.29760543408654283No Hit
CAAGAACAACTGGCCGGACGCCAACAAGGTGGGAGTGGGAGCATTCGGGC10250.29303128716494375No Hit
GTTGTCCTTGTGCGGGTTGAGGTCCCAATCTGGATTTTCTGAGTTGGCTT10100.2887430244259445No Hit
GCATCGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAACAAG10100.2887430244259445No Hit
CCTCCAGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA9900.2830253407739457No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAAGGGACTCAAGATG9530.2724476260177477No Hit
CTCCCCATGGGGGACTGTTGGGATGGAGCCCTCAGGCTCAGGGCATACTC9310.26615817400054886No Hit
GACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGGATCGG9060.2590110694355503No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT9040.2584393010703504No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCATCCCAACAGTCCCCCATG8440.24128625011435367No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT8220.2349967980971549No Hit
GTCCTCTACTTCCGGGATCATCAACAACCAGCACCGGACCATGCAAAACC8150.23299560881895526No Hit
CATGTGGAGGGATGAACCCTGGCCCGAATGCTCCCACTCCCACCTTGTTG8100.23156618790595554No Hit
GCCTAATCATCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8020.22927911444515597No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGGTTACGT7270.2078378007501601No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7260.20755191656756014No Hit
CTGTAAACGGAACCTCTTTGTTTCCCTCATGTTGCTGTACAAAACCTACG7170.20497895892416063No Hit
CTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA7040.20126246455036134No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC6970.19926127527216175No Hit
ATGCTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA6880.19668831762876227No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6510.18611060287256426No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG6370.18210822431616505No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT6310.18039291922056536No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6170.17639054066416615No Hit
CATCTCATCAGGACTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGGTTT6130.17524700393376635No Hit
ACAAGTTGGTGAGTGACTGGAGATTTGGGACTGCGAATTTTGGCCAAGAC6090.17410346720336659No Hit
ATCCCGGAAGTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGA6060.17324581465556674No Hit
GCCTAGTCATCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6040.17267404629036687No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA5970.17067285701216722No Hit
GTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA5840.16695636263836794No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA5800.16581282590796817No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGAAGTGGAA5730.16381163662976855No Hit
CCTGCTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCC5700.1629539840819687No Hit
GCCTAGCAGCCATGGGAAGGATGTATACTTGCGGGAGAGCACGACAGAAT5530.15809395297776965No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA5510.15752218461256975No Hit
GGGCAGCAAACCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCA5440.15552099533437014No Hit
CCAACCTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCG5430.1552351111517702No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT5410.15466334278657032No Hit
GATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCAGTCCTCGATAAGAT5400.15437745860397037No Hit
GTCCTCGATAAGATTGACGATATGGCAGAGACAGTATTCTGAGCAGGGCT5260.1503750800475711No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT5170.14780212240417162No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGAAGTGGAACTCCACCACTTT5110.14608681730857195No Hit
GTTACATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5100.145800933125972No Hit
GGAAAGTACAGGGTCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGT5070.14494328057817218No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT5050.14437151221297226No Hit
CAGCCGGTCTGGGGCAAAACTCATCGGGACTGACAATTCTGTCGTGCTCT5010.1432279754825725No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC4970.14208443875217272No Hit
GTCTTTGGGCATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT4930.14094090202177292No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4880.1395114811087732No Hit
ATGCCTGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCGACTTACAAA4850.13865382856097339No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4830.1380820601957735No Hit
CTGAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCC4820.13779617601317354No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA4780.13665263928277377No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT4760.13608087091757387No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG4680.1337937974567743No Hit
ATTGGGTGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAAATCAA4620.13207849236117464No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAG4590.1312208398133748No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA4550.13007730308297502No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC4520.1292196505351752No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4520.1292196505351752No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA4500.1286478821699753No Hit
GTATACATCCTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCTTGC4490.12836199798737535No Hit
TCCTAGTACAAAGACCTTTAACCTCATCTCCTCCCCCAACTCCTCCCACT4460.1275043454395755No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4430.1266466928917757No Hit
GGAAGGTACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGT4370.124931387796176No Hit
GACCGGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGGATCGG4370.124931387796176No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG4360.12464550361357607No Hit
GTCCGGTGCTGGTTGTTGATGATCCCGGAAGTAGAGGACAAACGGGCAAC4300.1229301985179764No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG4290.12264431433537644No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT4220.12064312505717684No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT4160.11892781996157716No Hit
GGTATACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCCGT4130.11807016741377734No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4110.11749839904857745No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4070.11635486231817765No Hit
TACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGG4010.11463955722257799No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG4000.11435367303997805No Hit
CTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGCA3990.1140677888573781No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA3980.11378190467477817No Hit
GTCCGGAAGACGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC3960.11321013630957825No Hit
GGTCTGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGC3860.1103512944835788No Hit
TTGCCGCCCCTTTCACGCAATGTGGATATCCTGCTTTAATGCCTTTATAT3830.10949364193577897No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT3830.10949364193577897No Hit
CTTCGTTACCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGCACC3770.10777833684017932No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG3750.1072065684749794No Hit
GGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAGGTAATTTGGAAGAT3740.10692068429237947No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT3720.10634891592717957No Hit
AGCATCGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAACAA3710.10606303174457964No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG3650.10434772664897997No Hit
GGGCAAAACTCATCGGGACTGACAATTCTGTCGTGCTCTCCCGCAAGTAT3640.10406184246638No Hit
GTTTCGCCCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCA3640.10406184246638No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC3630.10377595828378007No Hit
GTATACCAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG3620.10349007410118012No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG3610.10320418991858019No Hit
GTCTTCGATAAGATTGACGATATGGCAGAGACAGTATTCTGAGCAGGGCT3540.10120300064038058No Hit
GGGTAAAGGTTCAGATACTGGTTACGTAAAAAGGCTTTGTAAGTCGGCGA3540.10120300064038058No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG3530.10091711645778062No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCGACTTACAAAGCC3530.10091711645778062No Hit
GAACAGTGAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCAATCTTA3500.10005946390998079No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)