FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349792
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT18560.5306010429054981No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT16890.4828583844113073No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG15550.4445499039429146No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC14850.42453801116091844No Hit
GTACCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA14760.421965053517519No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA12090.34563397676333363No Hit
CCCAGAGACAACAGAAAATTGGTAACAGCGGCATAAAGGGACTCAAGATG11950.3416315982069344No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10960.3133290641295398No Hit
ATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA10930.31247141158174No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10380.29674778153874304No Hit
CCCAAAGACAACAGAAAATTGGTAACAGCGGCATAAAGGGACTCAAGATG10160.29045832952154427No Hit
CCTCCAGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA9170.2621557954441497No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAAGGGACTCAAGATG8830.2524357332357515No Hit
CCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTGCTGGTTGTTGA8670.2478615863141524No Hit
GCATCGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAACAAG8620.2464321654011527No Hit
GTCCTCTACTTCCGGGATCATCAACAACCAGCACCGGACCATGCAAAACC8490.24271567102735342No Hit
CTCCCCATGGGGGACTGTTGGGATGGAGCCCTCAGGCTCAGGGCATACTC8340.2384274082883542No Hit
CAAGAACAACTGGCCGGACGCCAACAAGGTGGGAGTGGGAGCATTCGGGC8290.23699798737535452No Hit
GTTGTCCTTGTGCGGGTTGAGGTCCCAATCTGGATTTTCTGAGTTGGCTT8180.2338532613667551No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8170.23356737718415516No Hit
GACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGGATCGG8100.23156618790595554No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCATCCCAACAGTCCCCCATG8030.2295649986277559No Hit
GCCTAATCATCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7750.22156024151495746No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7680.21955905223675784No Hit
CTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA7460.21326960021955904No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7390.21126841094135942No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG7080.20240600128076117No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG7070.2021201170981612No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC6870.1964024334461623No Hit
CATGTGGAGGGATGAACCCTGGCCCGAATGCTCCCACTCCCACCTTGTTG6790.19411535998536272No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6750.19297182325496295No Hit
CCTGCTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCC6720.1921141707071631No Hit
ATGCTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA6470.1849670661421645No Hit
CATCTCATCAGGACTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGGTTT6470.1849670661421645No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGGTTACGT6340.1812505717683652No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6290.17982115085536549No Hit
CTGTAAACGGAACCTCTTTGTTTCCCTCATGTTGCTGTACAAAACCTACG6250.1786776141249657No Hit
GCCTAGTCATCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5870.1678140151861678No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA5780.16524105754276827No Hit
GTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA5740.1640975208123685No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT5630.1609527948037691No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGAAGTGGAA5450.1558068795169701No Hit
GTATACATCCTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCTTGC5450.1558068795169701No Hit
ACAAGTTGGTGAGTGACTGGAGATTTGGGACTGCGAATTTTGGCCAAGAC5300.1515186167779709No Hit
GATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCAGTCCTCGATAAGAT5280.15094684841277103No Hit
GCCTAGCAGCCATGGGAAGGATGTATACTTGCGGGAGAGCACGACAGAAT5210.14894565913457142No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT5120.1463727014911719No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT5100.145800933125972No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA5060.14465739639557224No Hit
ATCCCGGAAGTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGA5060.14465739639557224No Hit
GTCCTCGATAAGATTGACGATATGGCAGAGACAGTATTCTGAGCAGGGCT4960.14179855456957277No Hit
GTCTTTGGGCATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT4940.14122678620437287No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGAAGTGGAACTCCACCACTTT4930.14094090202177292No Hit
GGTATACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCCCGT4920.140655017839173No Hit
GTTACATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4890.13979736529137315No Hit
CCAACCTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCG4880.1395114811087732No Hit
GGAAAGTACAGGGTCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGT4780.13665263928277377No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC4750.13579498673497392No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAG4650.13293614490897449No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG4570.13064907144817492No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA4490.12836199798737535No Hit
ATGCCTGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCGACTTACAAA4460.1275043454395755No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4450.12721846125697558No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4430.1266466928917757No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4280.1223584301527765No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT4280.1223584301527765No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT4250.12150077760497667No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4210.1203572408745769No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC4210.1203572408745769No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA4190.11978547250937702No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4160.11892781996157716No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA4140.11835605159637727No Hit
GGGCAGCAAACCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCA4140.11835605159637727No Hit
CAGCCGGTCTGGGGCAAAACTCATCGGGACTGACAATTCTGTCGTGCTCT4130.11807016741377734No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA4110.11749839904857745No Hit
ATTGGGTGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAAATCAA4100.1172125148659775No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT4100.1172125148659775No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG4070.11635486231817765No Hit
CTGAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCC4010.11463955722257799No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA3980.11378190467477817No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA3880.1109230628487787No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA3870.11063717866617877No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3850.11006541030097886No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG3850.11006541030097886No Hit
GTCCGGTGCTGGTTGTTGATGATCCCGGAAGTAGAGGACAAACGGGCAAC3850.11006541030097886No Hit
TACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGG3840.10977952611837892No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG3810.10892187357057909No Hit
GACCGGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGGATCGG3790.1083501052053792No Hit
CTTCGTTACCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGCACC3780.10806422102277925No Hit
GAACAGTGAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCAATCTTA3750.1072065684749794No Hit
GGAAGGTACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGT3730.10663480010977952No Hit
GGTCTGGTCTGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGC3670.10491949501417987No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG3660.10463361083157992No Hit
ATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTG3640.10406184246638No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT3600.10291830573598024No Hit
TTGCCGCCCCTTTCACGCAATGTGGATATCCTGCTTTAATGCCTTTATAT3590.10263242155338029No Hit
GTATACCAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG3590.10263242155338029No Hit
CTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGCA3580.10234653737078035No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG3500.10005946390998079No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)