FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418193
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC21460.5131601915861814No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGAAGTCCAA16760.4007718924037466No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13260.3170784781189546No Hit
CCTCCTGGATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12500.2989050510171141No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC12040.2879053451396843No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTAATGGGTCACCATATT11810.28240549220096944No Hit
CTTTTATATAAGATCCCCGCCTCCCAAAGGGTGTGTAAATAATGTCGCGT10420.2491672505278663No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG10200.24390652162996512No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG9270.22166798583429181No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT9230.22071148967103704No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA9110.21784200118127278No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8660.20708141934465668No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATT8600.20564667509977452No Hit
GTCCGGTGCTGGTTGTCGATGATCCTGGAATTAGAGGACAAACGGGCAAC8120.19416872114071734No Hit
GGACTCGTGCATGTTTTGCATGGTCCGGTGCTGGTTGTCGATGATCCTGG7980.19082098456932564No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7980.19082098456932564No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA7600.18173427101840536No Hit
TCCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7530.18006040273270954No Hit
CCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCACTCACC7480.17886478252864108No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7460.1783865344470137No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG7370.17623441807969048No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTAGATGCTGGATCTTC7250.17336492958992616No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7230.1728866815082988No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTTTTCAAGCCTCCAAGCTGTGC7120.17025631705934818No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6890.1647564641206333No Hit
TTCCCCCACTGTCTGGCTTTCAGTTACATGGATGATGTGGTTTTGGGGGC6880.1645173400798196No Hit
ACACACGGGTGTTCCCCCTAGAAAACTGAGAGAAGTCCACCACGAGTCTA6750.16140872754924163No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6700.16021310734517316No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6700.16021310734517316No Hit
CCCTTTGGGAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC6650.1590174871411047No Hit
CCCAAAGGGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA6650.1590174871411047No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCGACAACC6340.15160464187588027No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6300.1506481457126255No Hit
GTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT6230.14897427742692967No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC6080.1453874168147243No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAA6070.14514829277391061No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6010.14371354852902848No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG5970.14275705236577368No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGACGACTGGGGA5900.14108318408007786No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5900.14108318408007786No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA5830.13940931579438204No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT5670.13558333114136295No Hit
CCCGAGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCACTCACC5650.13510508305973556No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5610.13414858689648082No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA5580.13343121477403974No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGACGACTGGGGACCCTG5580.13343121477403974No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5570.13319209073322605No Hit
GGAACATATTGTTCAAAAAATCAAAATGTGTTTTAGAAAACTTCCTGTAA5560.13295296669241236No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGGATATAGACCACCAAATGCC5540.13247471861078497No Hit
GGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATCATCCATG5510.13175734648834392No Hit
GTACAGCAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG5490.1312790984067165No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG5480.13103997436590284No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5440.13008347820264804No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT5360.12817048587613855No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5340.12769223779451114No Hit
CTGTAAGGGGCCCTACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5290.12649661759044267No Hit
GCCTTAGAGTCTCCGGAACATTGTTCATCTCACCATACTGCACTCAGGCA5230.12506187334556054No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5200.12434450122311948No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATGTAACTGAAAGCCAGA5190.12410537718230577No Hit
TATCCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG5170.1236271291006784No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5170.1236271291006784No Hit
CCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTTGGTCTTCCAAAC5160.12338800505986469No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT5080.12147501273335518No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC5050.1207576406109141No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA5050.1207576406109141No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5030.12027939252928672No Hit
CATTTAATGGGTCACCATATTCTTGGGAACAAGATCTACAGCATGGGAGG5020.12004026848847302No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGA4990.11932289636603195No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTATGGACGGAAACTGC4950.1183664002027772No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTAG4950.1183664002027772No Hit
CTTGACTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTAT4860.11621428383545396No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4850.11597515979464028No Hit
CCTTATGGGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4810.11501866363138549No Hit
GAATACAGGTGCAGTTTCCGTCCATAGGTTTTGTACAGCAACATGAGGGA4750.11358391938650336No Hit
CACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACAA4740.11334479534568967No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4730.11310567130487599No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4730.11310567130487599No Hit
GCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA4700.11238829918243491No Hit
GTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGAACAATATGTT4690.1121491751416212No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA4680.11191005110080753No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4670.11167092705999383No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4660.11143180301918013No Hit
ATACTGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAATCTA4640.11095355493755275No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT4630.11071443089673907No Hit
ATATCCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4560.10904056261104322No Hit
CTCTTTTATATAAGATCCCCGCCTCCCAAAGGGTGTGTAAATAATGTCGC4520.10808406644778847No Hit
GTACAGGGTCCCCAGTCGTCGATAAGATTGACGATATGGCAGAGACAGTA4510.10784494240697476No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTGACGC4490.10736669432534739No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC4470.10688844624372No Hit
GTACAAAACCTATGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT4460.10664932220290631No Hit
GGGTAAAGGTTCAGATACTGTTGACGCAGAAAGGCCTTGTAAGTTGGCGA4430.10593195008046524No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA4430.10593195008046524No Hit
GGATATCCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4430.10593195008046524No Hit
ATAAAAGAGAGTCCACACGTAGCGCCTCATTTAATGGGTCACCATATTCT4380.10473632987639679No Hit
GCCGATTGGTGGAGGCAGGAGGAGGAGCTGCTGGCACAGTTGTGAGTAGG4370.1044972058355831No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGG4370.1044972058355831No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4330.10354070967232834No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGG4300.10282333754988726No Hit
GAATATGGTGACCCATTAAATGAGGCGCTACGTGTGGACTCTCTTTTATA4260.10186684138663248No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT4250.1016277173458188No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG4230.10114946926419142No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)