FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418193
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC19170.4584007862398462No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGAAGTCCAA16070.3842723335876019No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13200.3156437338740725No Hit
CCTCCTGGATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11900.28455760856829265No Hit
CTTTTATATAAGATCCCCGCCTCCCAAAGGGTGTGTAAATAATGTCGCGT11240.268775421874589No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTAATGGGTCACCATATT10990.26279732085424673No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10400.24868900244623895No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG9830.2350589321198585No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG9530.22788521089544778No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA8860.21186390016093049No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8750.2092335357119799No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATT8740.2089944116711662No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7620.18221251910003275No Hit
GTCCGGTGCTGGTTGTCGATGATCCTGGAATTAGAGGACAAACGGGCAAC7570.18101689889596428No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTAGATGCTGGATCTTC7550.18053865081433693No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT7520.17982127869189585No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA7360.1759952940388768No Hit
TCCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7360.1759952940388768No Hit
CCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCACTCACC7080.16929982089609344No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTTTTCAAGCCTCCAAGCTGTGC7010.1676259526103976No Hit
CCCAAAGGGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA6790.1623652237124964No Hit
GGACTCGTGCATGTTTTGCATGGTCCGGTGCTGGTTGTCGATGATCCTGG6670.15949573522273208No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6470.15471325440645825No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6380.15256113803913504No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGACGACTGGGGA6310.15088726975343922No Hit
GTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT6290.1504090216718118No Hit
TTCCCCCACTGTCTGGCTTTCAGTTACATGGATGATGTGGTTTTGGGGGC6290.1504090216718118No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCGACAACC6240.14921340146774334No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6200.1482569053044886No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6150.14706128510042013No Hit
CCCTTTGGGAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC6040.14443092065146954No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5950.14227880428414633No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5890.14084406003926417No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5880.14060493599845048No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5880.14060493599845048No Hit
CCCGAGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCACTCACC5880.14060493599845048No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC5860.1401266879168231No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5850.1398875638760094No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAA5760.1377354475086862No Hit
ACACACGGGTGTTCCCCCTAGAAAACTGAGAGAAGTCCACCACGAGTCTA5690.13606157922299034No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5530.13223559456997128No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG5470.13080085032508912No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG5430.12984435416183437No Hit
GCCTTAGAGTCTCCGGAACATTGTTCATCTCACCATACTGCACTCAGGCA5310.1269748656720701No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA5240.12530099738637424No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT5230.12506187334556054No Hit
GGAACATATTGTTCAAAAAATCAAAATGTGTTTTAGAAAACTTCCTGTAA5210.12458362526393316No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC5190.12410537718230577No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGACGACTGGGGACCCTG5170.1236271291006784No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA5150.12314888101905101No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5090.12171413677416885No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5060.12099676465172778No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTAG5040.1205185165701004No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT5030.12027939252928672No Hit
CTGTAAGGGGCCCTACAAATTGCTTACATCTATCAACAATGTCCTCCTGC4990.11932289636603195No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4900.11717077999870873No Hit
CCTTATGGGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4900.11717077999870873No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGGATATAGACCACCAAATGCC4870.11645340787626765No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGA4810.11501866363138549No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTATGGACGGAAACTGC4800.11477953959057183No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT4790.11454041554975812No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT4790.11454041554975812No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4760.11382304342731704No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT4750.11358391938650336No Hit
GGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATCATCCATG4750.11358391938650336No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4710.11262742322324859No Hit
GTACAGCAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG4680.11191005110080753No Hit
GTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGAACAATATGTT4670.11167092705999383No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC4660.11143180301918013No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA4650.11119267897836645No Hit
CTTGACTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTAT4610.11023618281511167No Hit
GTACAAAACCTATGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT4600.109997058774298No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT4590.1097579347334843No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT4570.10927968665185692No Hit
TATCCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG4560.10904056261104322No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC4550.10880143857022954No Hit
CATTTAATGGGTCACCATATTCTTGGGAACAAGATCTACAGCATGGGAGG4540.10856231452941584No Hit
GTACAGGGTCCCCAGTCGTCGATAAGATTGACGATATGGCAGAGACAGTA4530.10832319048860216No Hit
GAATACAGGTGCAGTTTCCGTCCATAGGTTTTGTACAGCAACATGAGGGA4520.10808406644778847No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGG4490.10736669432534739No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA4460.10664932220290631No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTGACGC4460.10664932220290631No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA4460.10664932220290631No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAATTGGGGGAGGAGGTTA4450.10641019816209263No Hit
CACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACAA4440.10617107412127892No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGG4430.10593195008046524No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4410.10545370199883786No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG4400.10521457795802418No Hit
CTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA4390.10497545391721047No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4370.1044972058355831No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4370.1044972058355831No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT4350.10401895775395571No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATGTAACTGAAAGCCAGA4320.10330158563151463No Hit
ATATCCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4310.10306246159070094No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4290.10258421350907355No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4260.10186684138663248No Hit
CCCCTATCTTATCAACACTTCCGGAAACTGCTGTTGTTAGACAAAGACGC4210.10067122118256402No Hit
GTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA4210.10067122118256402No Hit
GGGTAAAGGTTCAGATACTGTTGACGCAGAAAGGCCTTGTAAGTTGGCGA4190.10019297310093665No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)