FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421116
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAGGGAGCGGCAAACCCCAAAAGACCCACAATTCTTTGACATACT15870.3768557832046277No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA15790.37495606911159873No Hit
CCCTTAACTTCATGGGTTATGTAATTGGAAGTTGGGGTACCTTGCCACAG15560.36949439109414034No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACA14380.3414736082219626No Hit
CCCCAAGAGATTGCCGTGTGGTGGAGTGAACCCCGGCCCGAATGATCCCA14190.3369617872510187No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG14090.3345871446347325No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT13970.33173757349518895No Hit
AATCTGGATTGTTTGAATTGGCTCCGAATGCCGGGTCCAACTGGTGATCG13230.31416521813467074No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC12100.28733175657063614No Hit
CCTCCCGCTTATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA12000.28495711395434986No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11140.26453518745428817No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT11120.26406025893103086No Hit
CTCTTAGGGTGGAGCCCTCAGGCTCAGGGCATATTGACAACGGTACCAGC11080.2631104018845164No Hit
TACCCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG10830.25717379534380075No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTTGTAGTTGATGTTCCTGG10670.25337436715774275No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG9330.22155415609950704No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9250.21965444200647802No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC9200.21846712069833488No Hit
AGTTCAGGGAGTAGCCCCAACGTTTGGTTTTAGTAGGGTTTAAATGTATA8980.21324290694250517No Hit
CCCTAAGAGATTGCCGTGTGGTGGAGTGAACCCCGGCCCGAATGATCCCA8840.2099184072797044No Hit
CTCTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGACAACGGTACCAGC8760.20801869318667543No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTAGTAGGGTTTAAATGTATA8700.20659390761690366No Hit
CCCTGAACTTCATGGGTTATGTAATTGGAAGTTGGGGTACCTTGCCACAG8600.20421926500061743No Hit
ATCCCGATTGGGACTTCAACCCCAACAAGGATCAATGGCCAGCGGCAAAC8500.20184462238433115No Hit
ATTTTGTGGTCTATAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA8110.1925835161808148No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA7860.18664690964009917No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC7560.17952298179124043No Hit
GATTAGGATAGAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCA7560.17952298179124043No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC7480.17762326769821143No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC7250.17216158968075304No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG7200.17097426837260993No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC7080.16812469723306642No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATCATGCCTGCTAGG7050.16741230444818053No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7040.16717484018655193No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG7020.16669991166329468No Hit
CATTTAAACCCTACTAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6860.16290048347723668No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA6750.16028837659932182No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6610.15696387693652106No Hit
CTTATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCA6520.15482669858186343No Hit
TGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCAT6480.15387684153534892No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG6460.1534019130120917No Hit
GTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAATCTT6370.15126473465743406No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6360.15102727039580544No Hit
ATCCTAATCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT6340.15055234187254818No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAT6280.14912755630277644No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6250.14841516351789055No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGATAGGACAACAGAGT6220.1477027707330047No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6190.1469903779481188No Hit
GTCCCGTGCTTGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6070.1441408068085753No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT6060.1439033425469467No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC6030.1431909497620608No Hit
CCACAAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG6000.14247855697717493No Hit
ACCAGGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACA5990.1422410927155463No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTAGTAGGGTTTAAATGTATAC5950.1412912356690318No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATAACCCATGAA5840.13867912879111693No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5840.13867912879111693No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT5770.13701687895971656No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC5700.1353546291283162No Hit
ATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC5580.13250505798877268No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5530.13131773668062957No Hit
GTGAGATCCCAGAGTGAGGGGCCTATACTTTCCTGCTGGTGGCTCCAGTT5530.13131773668062957No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5530.13131773668062957No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5450.12941802258760057No Hit
CCGCCGACACATCCAGCGATAACCAGGACAAATTGGAGGACAAGAGGTTG5450.12941802258760057No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGATT5420.1287056298027147No Hit
CCCCAACAAGGATCAATGGCCAGCGGCAAACCAGGTAGGAGTGGGATCAT5300.12585605866317118No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5210.12371888030851357No Hit
GTCCAAGGGATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC5140.1220566304771132No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC5120.12158170195385595No Hit
ATCCAGATTGGGACTTCAACCCCAACAAGGATCAATGGCCAGCGGCAAAC5120.12158170195385595No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5100.1211067734305987No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5100.1211067734305987No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT5090.12086930916897008No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACTCATGCGCTGATGGCC5000.11873213081431244No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5000.11873213081431244No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGGTTCCCGAGATTGAGATCT5000.11873213081431244No Hit
ATGTATCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCT5000.11873213081431244No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4990.11849466655268381No Hit
GGTTTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGGAGTTCCACTGCAT4980.1182572022910552No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG4980.1182572022910552No Hit
CCCAAAGTGAGGGGCCTATACTTTCCTGCTGGTGGCTCCAGTTCCGGAAC4970.11801973802942657No Hit
ATCCCGATTGGGACTTCAACCCCAAAAAGGATCAATGGCCAGCGGCAAAC4960.11778227376779794No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA4960.11778227376779794No Hit
CTCAATTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCAC4960.11778227376779794No Hit
GTGGAACTCCAGCACATTCCACCAAGCTCTGCTAGATCCCAGAGTGAGGG4950.11754480950616933No Hit
GCCTAATCATCTCATGTTCATGTTCCACTGTTCAAGCCTCCAAGCTGTGC4950.11754480950616933No Hit
TATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATG4940.1173073452445407No Hit
CACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGGAGTTCCACTGCA4930.11706988098291206No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATTAGACAACTATT4900.11635748819802619No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4890.11612002393639757No Hit
AGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAATCT4810.11422030984336858No Hit
TACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGC4800.11398284558173995No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG4780.1135079170584827No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4770.11327045279685406No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAGTATAGGCCCCT4750.11279552427359682No Hit
GGATAGAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCATTAAC4690.11137073870382506No Hit
CCTCCCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4680.11113327444219644No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4570.10852116756428158No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCCTCAT4520.10733384625613844No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG4500.1068589177328812No Hit
AGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA4400.10448427511659496No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4390.10424681085496633No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4380.10400934659333771No Hit
CATACTGCGGAACTCCTAGCGGCTTGTTTTGCTCGCAGCAGGTCTGGAGC4370.10377188233170907No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4370.10377188233170907No Hit
ATATGGGAGAGGCAGTAGTCGGAACAGGGTTTACTGTTCCGGAACTGGAG4370.10377188233170907No Hit
CTTCCAAACTAGGCATTATTTACATACTCTGTGGAAGGCTGGTATTTTAT4360.10353441807008044No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGTTGAGTTGATGAATCTGGCCAC4290.10187216823868007No Hit
GGATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4290.10187216823868007No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4270.10139723971542283No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4270.10139723971542283No Hit
CCGCCTCTGCTTTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCA4250.10092231119216558No Hit
TTATTACCCTGAACATATAGTTAATCATTACTTCCAAACTAGGCATTATT4230.10044738266890833No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)