FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421116
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAGGGAGCGGCAAACCCCAAAAGACCCACAATTCTTTGACATACT15650.371631569448798No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG15380.3652200343848251No Hit
CCCTTAACTTCATGGGTTATGTAATTGGAAGTTGGGGTACCTTGCCACAG15240.36189553472202435No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14940.3547716068731656No Hit
CCCCAAGAGATTGCCGTGTGGTGGAGTGAACCCCGGCCCGAATGATCCCA14070.33411221611147524No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACA12820.3044291834078971No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT12540.2977801840822956No Hit
AATCTGGATTGTTTGAATTGGCTCCGAATGCCGGGTCCAACTGGTGATCG12460.2958804699892666No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT10450.24815015340191302No Hit
CCTCCCGCTTATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA10450.24815015340191302No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10370.24625043930888402No Hit
CTCTTAGGGTGGAGCCCTCAGGCTCAGGGCATATTGACAACGGTACCAGC10310.24482565373911228No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTTGTAGTTGATGTTCCTGG10300.24458818947748362No Hit
CCCTAAGAGATTGCCGTGTGGTGGAGTGAACCCCGGCCCGAATGATCCCA9300.22084176331462116No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC9210.21870458495996353No Hit
TACCCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG9010.21395529972739105No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG9000.2137178354657624No Hit
CTCTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGACAACGGTACCAGC8710.2068313718785323No Hit
ATCCCGATTGGGACTTCAACCCCAACAAGGATCAATGGCCAGCGGCAAAC8700.20659390761690366No Hit
CCCTGAACTTCATGGGTTATGTAATTGGAAGTTGGGGTACCTTGCCACAG8690.20635644335527506No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8430.20018237255293078No Hit
AGTTCAGGGAGTAGCCCCAACGTTTGGTTTTAGTAGGGTTTAAATGTATA8330.19780772993664453No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC8050.19115873061104305No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTAGTAGGGTTTAAATGTATA7670.1821350886691553No Hit
ATTTTGTGGTCTATAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA7590.1802353745761263No Hit
GATTAGGATAGAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCA7380.17524862508192518No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC7370.17501116082029652No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA7270.1726365182040103No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG7160.17002441132609541No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7130.16931201854120953No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6970.16551259035515153No Hit
TGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCTCAT6950.1650376618318943No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATCATGCCTGCTAGG6810.16171316216909354No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC6780.16100076938420768No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG6710.1593385195528073No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6680.15862612676792143No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATAACCCATGAA6620.15720134119814969No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG6350.1507898061341768No Hit
CATTTAAACCCTACTAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6330.15031487761091955No Hit
CCGCCGACACATCCAGCGATAACCAGGACAAATTGGAGGACAAGAGGTTG6240.14817769925626192No Hit
ATCCTAATCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT6230.1479402349946333No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGATT6120.14532812811671844No Hit
GTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAATCTT6080.14437827107020393No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC6070.1441408068085753No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA6040.14342841402368944No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6010.14271602123880356No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGATAGGACAACAGAGT6000.14247855697717493No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5930.14081630714577456No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAT5900.1401039143608887No Hit
GTCCCGTGCTTGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5800.13772927174460245No Hit
ACCAGGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACA5750.1365419504364593No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA5730.13606702191320208No Hit
CCACAAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG5730.13606702191320208No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5710.1355920933899448No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT5660.13440477208180168No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC5660.13440477208180168No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5610.13321745077365857No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5590.1327425222504013No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5500.1306053438957437No Hit
GTCCAAGGGATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC5440.12918055832597194No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5370.12751830849457158No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGGTTCCCGAGATTGAGATCT5340.1268059157096857No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5220.1239563445701422No Hit
GCCTAATCATCTCATGTTCATGTTCCACTGTTCAAGCCTCCAAGCTGTGC5200.12348141604688495No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5190.12324395178525632No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTAGTAGGGTTTAAATGTATAC5180.1230064875236277No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5160.12253155900037044No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACTCATGCGCTGATGGCC5100.1211067734305987No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5090.12086930916897008No Hit
ATCCAGATTGGGACTTCAACCCCAACAAGGATCAATGGCCAGCGGCAAAC5070.12039438064571281No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT5020.1192070593375697No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAGTATAGGCCCCT5010.11896959507594108No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATTAGACAACTATT4970.11801973802942657No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACAC4930.11706988098291206No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG4830.11469523836662582No Hit
ATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC4820.11445777410499719No Hit
GTGGAACTCCAGCACATTCCACCAAGCTCTGCTAGATCCCAGAGTGAGGG4800.11398284558173995No Hit
CTCAATTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCAC4790.11374538132011133No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4780.1135079170584827No Hit
CTTATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCA4770.11327045279685406No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG4770.11327045279685406No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4750.11279552427359682No Hit
ATCCCGATTGGGACTTCAACCCCAAAAAGGATCAATGGCCAGCGGCAAAC4740.11255806001196819No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4720.11208313148871095No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4690.11137073870382506No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG4680.11113327444219644No Hit
GGATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4660.1106583459189392No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT4660.1106583459189392No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4590.10899609608753882No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4580.1087586318259102No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4550.10804623904102434No Hit
GGATAGAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCATTAAC4550.10804623904102434No Hit
ATGTATCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCTATGCCT4550.10804623904102434No Hit
CCCCAACAAGGATCAATGGCCAGCGGCAAACCAGGTAGGAGTGGGATCAT4540.10780877477939571No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC4540.10780877477939571No Hit
ATATGGGAGAGGCAGTAGTCGGAACAGGGTTTACTGTTCCGGAACTGGAG4520.10733384625613844No Hit
GGTTTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGGAGTTCCACTGCAT4510.10709638199450983No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4480.10638398920962396No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4480.10638398920962396No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG4450.10567159642473807No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCCTCAT4440.10543413216310944No Hit
AGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAATCT4380.10400934659333771No Hit
GGAACATATTGTACACAAAATCAAACAATGTTTTCGGAAACTTCCTATAA4340.1030594895468232No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA4290.10187216823868007No Hit
GGTAATAGCGGTAAAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCA4290.10187216823868007No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)