FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520198
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGGTC34960.6720517956624208No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA24420.4694366375879953No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22380.4302208005413323No Hit
GGAACCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG16290.3131499928873237No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG15810.30392273711163825No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15340.29488771583127965No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT14580.2802778941864444No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG14510.27893225271915695No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT13550.2604777411677861No Hit
TCTAAACTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA13190.25355729933602206No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC12050.2316425668687692No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT11870.22818234595288717No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG11750.22587553200896582No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA11570.2224153110930838No Hit
GTGCTGGTGTGAACAATATCTGCACCTTTACCCCGTTGCTCGGCAACGGT11510.2212619041211231No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT10900.20953559990618958No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT10900.20953559990618958No Hit
ATCTGCACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG10730.20626761348563433No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG10110.194349074775374No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGGTCATT10030.19281119881275974No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG9580.18416064652305467No Hit
CCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGA9430.181277129093153No Hit
GCCTAGCAGCCATGGAAAGGAAGTGAATTTCCGAGAGAGGACAACAGAGT9240.17762467368194418No Hit
GTGCTGGTGAACAGACCAACTTTTTCACCTCTGCCTAAATCATCTCTTGT9000.17301104579410148No Hit
ATCCTAAGCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAACTTAT8940.1718576388221408No Hit
CCTACAAATAGACCTATTGATTGGAAAGTATGTCAACGCATTGTGGGGCT8710.16743624542962487No Hit
GGAGTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG8330.16013133460720724No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC8330.16013133460720724No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC8250.158593458644593No Hit
CATTTAAACCCTGCTAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8230.15820898965393945No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8180.15724781717730557No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG8170.1570555826819788No Hit
GCTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG8150.15667111369132525No Hit
TGTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA8020.1541720652520771No Hit
GCTTACTACTGCTTCCCTGGATGTTGGGTCTTCCAAATTACCTCCCACCC7810.15013514085021473No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7800.14994290635488794No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC7790.14975067185956117No Hit
CCTACAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT7680.1476360924109666No Hit
GTAGTAAGCTATGTCAACGTTAATATGGGCCTAAAACTCCGACAATTATT7550.14513704397171845No Hit
GCATCAATGCTGGGTAACCACATTGTGTAAAGGGAGCGGCAAAGCCCAGA7520.14456034048573813No Hit
GTGGTGGAGTGAACCCTGGCCCGAAGGATCCCACTCCTACCTGGTGTGCC7450.14321469901845066No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT7420.1426379955324703No Hit
GACCAATTTATGCCTACAGCCTCCTAGTACAAAGGTCATTAATCTAAACT7390.14206129204648998No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTCAACG7290.1401389470932222No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA7130.13706319516799373No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG7100.13648649168201338No Hit
ATCTGACCCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG7010.13475638122407235No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6980.13417967773809203No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6930.13321850526145815No Hit
ATGTAGGCCCTCTTACAGTCAACGAAAAAAGGAGATTAAAATTGATTATG6930.13321850526145815No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG6860.13187286379417068No Hit
AATCTAAACTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTT6860.13187286379417068No Hit
ATTTATAGGAAGTTTTCGAAAACATTGTTTGATTTTGTGTACAATATGTT6620.12725923590632796No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG6620.12725923590632796No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC6540.12572135994371375No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC6520.1253368909530602No Hit
ATATATAATTGGAAGTTGGGGTACCTTACCACAGGAACATATTGTACACA6510.1251446564577334No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6500.12495242196240662No Hit
GCACGGGACCATGCAAGACCTGCACGGTTCCTGCTCAAGGAACCTCTATG6440.12379901499044595No Hit
GACCAACTTTTTCACCTCTGCCTAAATCATCTCTTGTTCATGTCCCACTG6400.12303007700913884No Hit
TCCTAGTACAAAGGTCATTAATCTAAACTCCTCCCCCAACTCCTCCCAGT6350.12206890453250493No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6230.11976209058858357No Hit
TTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCT6200.11918538710260325No Hit
GCACGGTTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA6190.11899315260727646No Hit
CTTCAAAGACTGTGTGTTTAAAGACTGGGAGGAGTTGGGGGAGGAGTTTA6150.11822421462596934No Hit
CCTTACCACAGGAACATATTGTACACAAAATCAAACAATGTTTTCGAAAA6150.11822421462596934No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6040.11610963517737477No Hit
CTCTTACAGTCAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG5860.11264941426149275No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTGTCTTCCAAAAGTAAGA5830.1120727107755124No Hit
CCCTTAACTTCATGGGATATATAATTGGAAGTTGGGGTACCTTACCACAG5820.11188047628018562No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC5810.11168824178485884No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTGAC5780.1111115382988785No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5750.11053483481289818No Hit
CCTTACCACAGGAACATATTGTACACAAAATCAAACAATGTTTTCGGAAA5670.10899695885028392No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5650.10861248985963036No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5630.1082280208689768No Hit
GCCTAAATCATCTCTTGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTG5600.10765131738299648No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC5570.10707461389701614No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGGTCATTAATCTAAACTCCTCC5570.10707461389701614No Hit
GTGGTAAGGTACCCCAACTTCCAATTATATATCCCATGAAGTTAAGGGAG5530.10630567591570902No Hit
ATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTG5470.10515226894374835No Hit
GAAAAAAGTCAGAAGGCAAAAAAGAGAGTAACTCCACAGAAGCTCCAAAT5420.10419109646711444No Hit
GCCTGAGGATGACTGTCTCTTAGAGGCGGAGAGATGGGAGTAGGCTGTCT5370.10322992399048055No Hit
ATATTAACGTTGACATAGCTTACTACTGCTTCCCTGGATGTTGGGTCTTC5360.10303768949515377No Hit
GTACAAAGGTCATTAATCTAAACTCCTCCCCCAACTCCTCCCAGTCTTTA5350.10284545499982699No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5350.10284545499982699No Hit
GACTTCTTTCCATCTATTCGCGATCTCCTCGACACCGCATCTGCCCTGTA5310.10207651701851987No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5290.10169204802786631No Hit
TCCCGATACAGGGCAGATGCGGTGTCGAGGAGATCGCGAATAGAAGGAAA5270.10130757903721276No Hit
TCTGCACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGT5260.10111534454188596No Hit
GCCTATATTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTT5240.1007308755512324No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)