FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204391
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9840.48143019995988084No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT9070.44375730829635357No Hit
GTGTAAAGGGTGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT8440.4129340332989222No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC8260.40412738329965603No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7970.3899388916341717No Hit
ATATAAGACCTTAGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC7400.3620511666364957No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6290.3077434916410214No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG6050.2960012916419999No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5100.2495217499792065No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAA4110.20108517498324288No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4080.1996173999833652No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC4080.1996173999833652No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTGAAACTCAGACAACTATT4040.19766036665019496No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC3750.18347187498471068No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG3730.18249335831812555No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3670.17955780831837018No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG3670.17955780831837018No Hit
ATCCTAACCGTACCAAATATTTACCCTTAGATAAAGGCATCAAACCTTAT3590.17564374165202967No Hit
GCTTACTACTGCTTCCCTGGATGTAGGGTCTTCCAAATTACTTCCCACCC3580.17515448331873715No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG3460.16928338331922638No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT3450.16879412498593382No Hit
TCCAAAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA3410.16683709165276356No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCCAACGGAACCTCTATG3380.1653693166528859No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC3320.16243376665313053No Hit
CTCTTACAGTTAACGAAAAAAGAAGATTAAAATTGATCATGCCTGCTAGG3280.16047673331996026No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT3280.16047673331996026No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3270.1599874749866677No Hit
CTCTTTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC3240.15851969998679002No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT3200.1565626666536198No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT3100.15167008332069415No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3090.1511808249874016No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT3060.1497130499875239No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG3010.14726675832106112No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC2980.1457989833211834No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG2950.14433120832130572No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA2920.14286343332142803No Hit
CTTATATAAAATACCAGCCTTCCAAAGAGTATGTAAATAATGCCTAGTTT2880.14090639998825782No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA2850.1394386249883801No Hit
CCCCAAAAGATTGCCGTGTGGTGGAGTGAACCCCGGCCCGAATGATCCCA2830.138460108321795No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA2810.13748159165520987No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGCGGGTCACCATATT2800.1369923333219173No Hit
ACATAGAGGTTCCGTTGGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG2780.1360138166553322No Hit
CTCTTATATAAGACCTTAGGCAAGACCTGGTGGGCGTTCACGGTGGTTTC2740.13405678332216192No Hit
CTCCAAAAGACACCAAATACTCAAGAACAACTTCTCTTCCAAAAGTAAGA2700.1320997499889917No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT2700.1320997499889917No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTTCTTTTTTCG2670.130631974989114No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG2660.13014271665582144No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG2650.1296534583225289No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG2650.1296534583225289No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG2640.1291641999892363No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT2630.12867494165594376No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG2630.12867494165594376No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC2630.12867494165594376No Hit
GCCTAATCATCTCATGTTCATGTTCCACTGTTCAAGCCTCCAAGCTGTGC2600.12720716665606607No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG2560.1252501333228958No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG2540.12427161665631069No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTAGGCAAGACCTGGTGGGCGTTC2520.12329309998972558No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC2500.12231458332314046No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG2490.1218253249898479No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTTCTTTTTTCGTTAAC2480.12133606665655533No Hit
TAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCT2480.12133606665655533No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC2460.12035754998997021No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA2460.12035754998997021No Hit
ATTTACATACTCTTTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT2450.11986829165667764No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA2440.11937903332338508No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG2430.11888977499009251No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGAGCATGGACATTGA2430.11888977499009251No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT2430.11888977499009251No Hit
ATCTAAGGGTAAATATTTGGTACGGTTAGGATAGAACCTAGCAGGCATGA2420.11840051665679996No Hit
GTACAAAGATCATTAACCGAATCTCCTCCCCCAACTCCTCCCAGTCCTTA2410.11791125832350739No Hit
GGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCG2330.1139971916571669No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG2330.1139971916571669No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC2320.11350793332387435No Hit
CCAAAAGACACCAAATACTCAAGAACAACTTCTCTTCCAAAAGTAAGACA2310.11301867499058178No Hit
ATTTATGCCTACAGCCTCCCAGTACAAAGATCATTAACCGAATCTCCTCC2290.11204015832399665No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG2290.11204015832399665No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA2280.1115508999907041No Hit
GGATACTAACATTGGGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA2280.1115508999907041No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG2270.11106164165741153No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG2250.1100831249908264No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG2250.1100831249908264No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA2240.10959386665753384No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC2240.10959386665753384No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC2240.10959386665753384No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAAT2230.10910460832424128No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC2220.10861534999094871No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT2180.10665831665777849No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGTAAATATTTGGTA2180.10665831665777849No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC2170.10616905832448592No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG2170.10616905832448592No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT2170.10616905832448592No Hit
GCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGCGG2150.10519054165790079No Hit
CTTCCAAACTAGGCATTATTTACATACTCTTTGGAAGGCTGGTATTTTAT2150.10519054165790079No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG2140.10470128332460824No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA2120.1037227666580231No Hit
CGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG2090.10225499165814542No Hit
TCCTTTGTCTACGTCCCGTCAGCGCTGAATCCCGCGGACGACCCGTCTCG2090.10225499165814542No Hit
TTATTATCCTGAACATATAGTTAATCATTACTTCCAAACTAGGCATTATT2080.10176573332485285No Hit
GTCCAAGGGATACTAACATTGGGGTTCCCGAGATTGAGATCTTCTGCGAC2070.10127647499156028No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG2070.10127647499156028No Hit
GAATAAAGCCCAGTGAAGTTTCCCACCTTATGAGTCCAAGGGATACTAAC2070.10127647499156028No Hit
GGTTATCGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCT2060.10078721665826773No Hit
CCTTATGAGTCCAAGGGATACTAACATTGGGGTTCCCGAGATTGAGATCT2060.10078721665826773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)