FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204391
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9870.4828979749597585No Hit
GTGTAAAGGGTGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT7920.38749259996770896No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC7810.38211075830149077No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT7740.37868594996844285No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7620.3728148499689321No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG6590.32242124163979824No Hit
ATATAAGACCTTAGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC5510.26958134164420156No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4480.2191877333150677No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4430.21674144164860487No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTGAAACTCAGACAACTATT4200.20548849998287597No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAA4200.20548849998287597No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC3680.18004706665166276No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG3610.1766222583186148No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG3520.17221893331898175No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3360.16439079998630077No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG3350.16390154165300821No Hit
CTTATATAAAATACCAGCCTTCCAAAGAGTATGTAAATAATGCCTAGTTT3310.16194450831983795No Hit
CTCTTTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC3300.1614552499865454No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG3270.1599874749866677No Hit
CGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG3230.15803044165349747No Hit
CTCTTACAGTTAACGAAAAAAGAAGATTAAAATTGATCATGCCTGCTAGG3230.15803044165349747No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT3070.15020230832081646No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC3050.14922379165423136No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCCAACGGAACCTCTATG3020.14775601665435367No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC3010.14726675832106112No Hit
GCTTACTACTGCTTCCCTGGATGTAGGGTCTTCCAAATTACTTCCCACCC2940.14384194998801317No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT2900.1418849166548429No Hit
CTCCAAAAGACACCAAATACTCAAGAACAACTTCTCTTCCAAAAGTAAGA2890.14139565832155035No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG2890.14139565832155035No Hit
ATCCTAACCGTACCAAATATTTACCCTTAGATAAAGGCATCAAACCTTAT2870.14041714165496524No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG2850.1394386249883801No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT2780.1360138166553322No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT2770.1355245583220396No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG2760.13503529998874705No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG2750.1345460416554545No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG2740.13405678332216192No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT2710.13258900832228424No Hit
TCCAAAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA2680.13112123332240658No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA2670.130631974989114No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC2650.1296534583225289No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC2640.1291641999892363No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG2620.1281856833226512No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTTCTTTTTTCGTTAAC2590.1267179083227735No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC2550.12476087498960325No Hit
ATTTACATACTCTTTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT2540.12427161665631069No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA2520.12329309998972558No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT2510.12280384165643302No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG2500.12231458332314046No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT2470.12084680832326276No Hit
GGTTGGGGCTTGGCCATGGGCCATCAGCGCATGCGTGGAACCTTTGTGGC2460.12035754998997021No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTTCTTTTTTCG2450.11986829165667764No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA2450.11986829165667764No Hit
ATCTAAGGGTAAATATTTGGTACGGTTAGGATAGAACCTAGCAGGCATGA2440.11937903332338508No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT2440.11937903332338508No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG2440.11937903332338508No Hit
CCCCAAAAGATTGCCGTGTGGTGGAGTGAACCCCGGCCCGAATGATCCCA2410.11791125832350739No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG2400.11742199999021483No Hit
GCCTAATCATCTCATGTTCATGTTCCACTGTTCAAGCCTCCAAGCTGTGC2380.1164434833236297No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG2380.1164434833236297No Hit
CTTCAAAGACTGTGTATTTAAGGACTGGGAGGAGTTGGGGGAGGAGATTC2360.11546496665704457No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA2330.1139971916571669No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG2330.1139971916571669No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTAGGCAAGACCTGGTGGGCGTTC2330.1139971916571669No Hit
ACATAGAGGTTCCGTTGGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG2320.11350793332387435No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT2310.11301867499058178No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG2310.11301867499058178No Hit
GAATAAAGCCCAGTGAAGTTTCCCACCTTATGAGTCCAAGGGATACTAAC2300.11252941665728922No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC2290.11204015832399665No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGCGGGTCACCATATT2290.11204015832399665No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC2270.11106164165741153No Hit
CTCTTATATAAGACCTTAGGCAAGACCTGGTGGGCGTTCACGGTGGTTTC2260.11057238332411896No Hit
GGATACTAACATTGGGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA2220.10861534999094871No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG2220.10861534999094871No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC2210.10812609165765615No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAAT2210.10812609165765615No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGTAAATATTTGGTA2210.10812609165765615No Hit
TCCTTTGTCTACGTCCCGTCAGCGCTGAATCCCGCGGACGACCCGTCTCG2180.10665831665777849No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA2130.10421202499131567No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA2110.10323350832473055No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA2100.10274424999143798No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT2090.10225499165814542No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA2090.10225499165814542No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGAGCATGGACATTGA2070.10127647499156028No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG2050.10029795832497516No Hit
GTCCAAGGGATACTAACATTGGGGTTCCCGAGATTGAGATCTTCTGCGAC2050.10029795832497516No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)