FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394496
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC25500.646394386761843No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA25040.6347339390006489No Hit
CCTCTCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA18310.4641365184944841No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC17230.436759815055159No Hit
GTTTAAGTCCCAATCTGGATTGTTTGAATTGGCTCTGAATGCAGGGTCCA17000.4309295911745619No Hit
CTCTCTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC15910.4032993997404283No Hit
TTAACCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCT13620.3452506489292667No Hit
CTATCAATAATATCCTCTTTTAAATGAATGTGAGGAAAAGAGGGAGTTTG13130.33282973718364695No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT12480.3163530175210902No Hit
CATTCTGTGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGG11870.3008902498377677No Hit
CCCACGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC11610.294299561972745No Hit
CCATCTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGA9900.2509531148604802No Hit
GAACACATAGTTAATCATTACTTCCAAACTAGGCATTATTTACATACTCT9670.24512289097988318No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCGTTGTATGCATGTAT9400.23827871512005191No Hit
GGATTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG9370.2375182511356262No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC9000.2281391953277093No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8750.22180199545749515No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGA8670.2197740914990266No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACCACAGAGT8620.21850665152498377No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8200.20786015574302402No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA7910.2005090038935756No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG7730.1959462199870214No Hit
GCTTTATATAGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA7540.19112994808565867No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA7450.18884855613238155No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG7370.18682065217391305No Hit
CCTCTGCTTTATATAGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCT7360.18656716417910446No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA7350.18631367618429592No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA7290.1847927482154445No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG7270.18428577222582737No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT7090.17972298831927322No Hit
ATTTCCGAGAGAGGACCACAGAGTTGTCGGTGCCGAGAATGTTTGCTCCA6570.16654161258922778No Hit
GCTTTGAGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTACTGTGG6530.1655276606099935No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG6530.1655276606099935No Hit
CCCAAGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC6450.163499756651525No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC6400.16223231667748217No Hit
TGGAAACCACCGTGAACGCCCGCCAGGTCTTGCCCAAGGTCTTACATAAG6380.16172534068786504No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACATTACCAC6260.15868348475016222No Hit
CTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC6190.15690906878650226No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT6060.1536137248539909No Hit
GATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTG5930.15031838092147956No Hit
ACACTTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA5920.150064892926671No Hit
GTATACATGCATACAACGGCATTAATGCTGGGTAACCACATTGTGTAAAG5890.1493044289422453No Hit
GTTGCGGTCCCAATCTGGATTGTTTGAATTGGCTCTGAATGCAGGGTCCA5840.14803698896820247No Hit
CTATATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATAT5840.14803698896820247No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC5780.14651606099935105No Hit
CATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGC5730.14524862102530822No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5700.14448815704088255No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5630.1427137410772226No Hit
TATTAACATTGACATAGCTTACTACTAATTCCCTGGATGCTGGATCTTCC5530.1401788611291369No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTTTCCATGC5480.13891142115509408No Hit
GTTAAGTGAGTAGCCCCAACGTTTGGTTTTAGCGGGGTTTAAATGTATAC5430.13764398118105126No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG5420.13739049318624272No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG5360.1358695652173913No Hit
CTCCCAGTACAAAGATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAG5290.13409514925373134No Hit
ACTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG5250.13308119727449708No Hit
GTGCTGGAGTTCCACTGCATGGCCTGAGGATGACTGTCTCTTAGAGGTGG5150.13054631732641142No Hit
CCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGAAGTATGCCTCA5150.13054631732641142No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5140.13029282933160286No Hit
CTATTAATAATATCCTCTTTTAAATGAATGTGAGGAAAAGAGGGAGTTTG5100.1292788773523686No Hit
GCACGGGACCATGCAAGACCTGCACGAATCCTGCTCAAGGAACCTCTATG5080.12877190136275146No Hit
GGATATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG5040.1277579493835172No Hit
CTCGAAAATACACCTCTTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGG5000.1267439974042829No Hit
GTACAAAGATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTA4970.12598353341985724No Hit
ACTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACATTACCA4920.12471609344581441No Hit
CTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATAT4900.12420911745619728No Hit
GTAAAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC4900.12420911745619728No Hit
TCTCATGTTCATGTCTCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4730.11989982154445165No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGGCAACGGTCAGGTC4640.11761842959117456No Hit
GTTCAAGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTGACGCAACCCCC4640.11761842959117456No Hit
GATCCATGGCCAGCGGCACACCAGGTAGGAGTGGGATCATTCGGGCCAGG4630.11736494159636598No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT4630.11736494159636598No Hit
ATATAGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4590.11635098961713174No Hit
ATCCTGATGTGGTGCTCTCCATCTTCGGTGCAGGGTCCCCAGTCCTCGAG4530.11483006164828033No Hit
GTATAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC4530.11483006164828033No Hit
CATTTAAACCCCGCTAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4500.11406959766385465No Hit
ATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGCA4480.11356262167423752No Hit
GGGATCATTCGGGCCAGGGTTCACTCCACCACACGGCAATCTTTTGGGGT4460.11305564568462037No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGGCAACGGTCAGGTC4460.11305564568462037No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC4450.11280215768981182No Hit
CAAGACCATATTGTCAACAAAATCAAACAATGTTTTCGGAAACTTCCTGT4420.11204169370538611No Hit
TAAGTGAGTAGCCCCAACGTTTGGTTTTAGCGGGGTTTAAATGTATACCC4420.11204169370538611No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4400.11153471771576898No Hit
CTTATATAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTTTCCA4370.11077425373134328No Hit
GTCTCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGAGGCATG4310.10925332576249189No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA4310.10925332576249189No Hit
GGTGCTCTCCATCTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACG4310.10925332576249189No Hit
GGACCGCAACCCCAAAAAGGATCAATGGCCAGCGGCACACCAGGTAGGAG4290.10874634977287477No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4290.10874634977287477No Hit
GCACTAAGGCAAGCTATTTTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4280.10849286177806619No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG4280.10849286177806619No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4240.10747890979883193No Hit
GTTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA4220.1069719338092148No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4210.10671844581440623No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA4190.1062114698247891No Hit
CCGAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTT4090.10367658987670343No Hit
GTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGG4080.10342310188189488No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4080.10342310188189488No Hit
TCTCGGGGCCGTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCG4060.10291612589227773No Hit
GTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTC4030.10215566190785205No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3980.10088822193380922No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG3970.10063473393900065No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC3960.1003812459441921No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)