FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394496
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC23160.5870781959766386No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA21020.5328317650876054No Hit
CCTCTCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA18080.4583062946138871No Hit
GTTTAAGTCCCAATCTGGATTGTTTGAATTGGCTCTGAATGCAGGGTCCA16280.41267845554834526No Hit
CTCTCTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC14340.36350178455548343No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT11890.3013972258273848No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC11880.30114373783257625No Hit
CTATCAATAATATCCTCTTTTAAATGAATGTGAGGAAAAGAGGGAGTTTG11820.29962280986372486No Hit
CATTCTGTGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGG10900.2763019143413368No Hit
GAACACATAGTTAATCATTACTTCCAAACTAGGCATTATTTACATACTCT8870.2248438513951979No Hit
TTAACCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACACACAGTCT8850.22433687540558078No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG8710.22078804347826086No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8690.22028106748864373No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCGTTGTATGCATGTAT8680.2200275794938352No Hit
CCATCTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGA8580.21749269954574954No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7960.20177644386761845No Hit
GCTTTATATAGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA7810.19797412394548997No Hit
GGATTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG7760.1967066839714471No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA7340.18606018818948736No Hit
CCTCTGCTTTATATAGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCT7330.18580670019467876No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA7130.18073694029850745No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG6850.17363927644386762No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACCACAGAGT6750.17110439649578196No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6660.16882300454250487No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6660.16882300454250487No Hit
TGGAAACCACCGTGAACGCCCGCCAGGTCTTGCCCAAGGTCTTACATAAG6610.16755556456846205No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG6530.1655276606099935No Hit
CTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACATTACCAC6520.16527417261518496No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6340.16071138870863078No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT6290.15944394873458795No Hit
CTCGAAAATACACCTCTTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGG6150.15589511680726803No Hit
CCCAAGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC6130.15538814081765087No Hit
ATTTCCGAGAGAGGACCACAGAGTTGTCGGTGCCGAGAATGTTTGCTCCA5960.15107884490590526No Hit
GCTTTGAGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTACTGTGG5920.150064892926671No Hit
GATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTG5910.14981140493186243No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5600.1419532770927969No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC5570.14119281310837117No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5570.14119281310837117No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGGCAACGGTCAGGTC5440.13789746917585985No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG5390.136630029201817No Hit
CTCTGTGGAAGGCGGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC5350.13561607722258276No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5330.1351091012329656No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5290.13409514925373134No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA5250.13308119727449708No Hit
ACACTTGGGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA5150.13054631732641142No Hit
GTTCAAGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTGACGCAACCCCC5130.1300393413367943No Hit
TATTAACATTGACATAGCTTACTACTAATTCCCTGGATGCTGGATCTTCC5110.12953236534717716No Hit
GCACGGGACCATGCAAGACCTGCACGAATCCTGCTCAAGGAACCTCTATG5080.12877190136275146No Hit
GTTGCGGTCCCAATCTGGATTGTTTGAATTGGCTCTGAATGCAGGGTCCA5000.1267439974042829No Hit
CTATATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATAT5000.1267439974042829No Hit
GTATACATGCATACAACGGCATTAATGCTGGGTAACCACATTGTGTAAAG4970.12598353341985724No Hit
GGATATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG4930.12496958144062296No Hit
TCTCGGGGCCGTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCG4910.12446260545100583No Hit
GTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTC4800.12167423750811161No Hit
ACTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACATTACCA4750.12040679753406879No Hit
GTTAAGTGAGTAGCCCCAACGTTTGGTTTTAGCGGGGTTTAAATGTATAC4690.1188858695652174No Hit
GTGCTGGAGTTCCACTGCATGGCCTGAGGATGACTGTCTCTTAGAGGTGG4680.11863238157040883No Hit
CTATTAATAATATCCTCTTTTAAATGAATGTGAGGAAAAGAGGGAGTTTG4670.11837889357560027No Hit
ATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGCA4660.1181254055807917No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4660.1181254055807917No Hit
CTCCCAGTACAAAGATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAG4620.11711145360155743No Hit
TCTCATGTTCATGTCTCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4620.11711145360155743No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA4610.11685796560674887No Hit
ACTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG4600.11660447761194029No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGGCAACGGTCAGGTC4490.11381610966904607No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC4470.11330913367942894No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4460.11305564568462037No Hit
CATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGC4460.11305564568462037No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG4450.11280215768981182No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4440.11254866969500324No Hit
GTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGG4440.11254866969500324No Hit
CCCCCAACTCCTCCCAGTCTTTAAACACACAGTCTTTGAAGTATGCCTCA4430.11229518170019469No Hit
GTACAAAGATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTA4410.11178820571057756No Hit
GTTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA4410.11178820571057756No Hit
CTGTATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATAT4390.11128122972096041No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT4390.11128122972096041No Hit
ATATAGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4360.11052076573653473No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4320.10950681375730045No Hit
ACTCTGTGGTCCTCTCTCGGAAATACACCTCTTTTCCATGGCTGCTAGGC4310.10925332576249189No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTTTCCATGC4290.10874634977287477No Hit
CAAGACCATATTGTCAACAAAATCAAACAATGTTTTCGGAAACTTCCTGT4280.10849286177806619No Hit
GTAAAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC4260.10798588578844906No Hit
CATTTAAACCCCGCTAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4250.10773239779364048No Hit
CTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACGGGGCGCACCT4220.1069719338092148No Hit
GTCTCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGAGGCATG4200.10646495781959765No Hit
GTATAAAGGGATTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC4200.10646495781959765No Hit
GGACCGCAACCCCAAAAAGGATCAATGGCCAGCGGCACACCAGGTAGGAG4170.10570449383517197No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4150.10519751784555484No Hit
GGGATCATTCGGGCCAGGGTTCACTCCACCACACGGCAATCTTTTGGGGT4140.10494402985074627No Hit
GATCCATGGCCAGCGGCACACCAGGTAGGAGTGGGATCATTCGGGCCAGG4140.10494402985074627No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4070.10316961388708631No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4060.10291612589227773No Hit
ATCCTGATGTGGTGCTCTCCATCTTCGGTGCAGGGTCCCCAGTCCTCGAG4060.10291612589227773No Hit
GGTGCTCTCCATCTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACG4050.10266263789746918No Hit
CTGCTAGGCTGCGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4050.10266263789746918No Hit
ATGTTCTTGTGGTAATGTACCCCAACTTCCAATTACATATCCCATGAAGT4010.10164868591823492No Hit
CCCACGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC3990.10114170992861778No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)