FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508523
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT24260.477067900566936No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG23860.4692019829978192No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA23440.4609427695502465No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC23000.4522902602242179No Hit
CCTTTGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT22950.45130702052807836No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT20600.405094754809517No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC18790.3695014778092633No Hit
CCTCCCGCGTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17710.3482635003726478No Hit
TACCCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG13510.2656713658969211No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACCGAGT12650.24875964312331988No Hit
TCCGCAAACATTGTTTGATTTTTTGTACAATATGTTCCTGTGGCAAGGTA11970.23538758325582126No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11940.2347976394381375No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT11300.22221217132755056No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAGGTATAGGCCCCT11270.22162222750986682No Hit
ACCACACGGCAATCTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGA10810.21257642230538246No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG10760.21159318260924287No Hit
GTAGTAAACTATGTCAATGTTAATATGGGCCTAAAAATGAGACAACTATT10650.20943005527773573No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC10610.20864346352082405No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG10330.20313732122244224No Hit
GTTTACTACTGATTCCCTGGATGCCGGGTCTTCCAAATTACTTCCCACCC10150.1995976583163397No Hit
TCTCTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG10150.1995976583163397No Hit
GCACTAAGGCAAGCTATACTATGTTGGAGCGAGGTAATGAATCTGGCCAC9970.1960579954102371No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG9960.19586134747100917No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG9850.19369822013950205No Hit
CTACAAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA9750.19173174074722285No Hit
ACAAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC9680.19035520517262738No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC9590.18858537371957612No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA9480.18642224638806898No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA9460.18602895050961313No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT9450.18583230257038522No Hit
GTATGTAAATAATGCCTGGTTTGGAAGTAATGATTAACTATATGTTCAGG9300.1828825834819664No Hit
CTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA9150.17993286439354758No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC9140.17973621645431967No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG9040.17776973706204047No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCGGCTAGG9000.1769831453051288No Hit
ATGTAAGACCTTAGGCAAGACTTGGCGGGCGTTCACGGTGGTCTCCATGC8920.1754099617913054No Hit
GTTTTCGGAAACTTCCTGTCAATAGGCCTATTGATTGGAAAGTATGTCAA8900.1750166659128496No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAGCATTGTTCACCTCACCATACAG8890.17482001797362165No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC8830.17364013033825412No Hit
ATTCTTTGACATACTTTCCAATCAATAGGCCTATTGACAGGAAGTTTCCG8560.16833063597910025No Hit
CTCCAAAGGACACCAAATACTCAAGAACCGTTTCTCTTCCAAAAGTAAGA8340.164004381316086No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC8220.16164460604535094No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG8210.16144795810612303No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG8140.1600714225315276No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA8140.1600714225315276No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC8090.15908818283538798No Hit
GCGCTAAATCCTGCGGACGACCCGTCCCGGGGCCGTTTGGGGCTCTACCG8020.15771164726079254No Hit
GTATAGGCCCCTTACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG8000.15731835138233669No Hit
CATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACC7970.15672840756465295No Hit
TGCAAAACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG7920.1557451678685133No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7890.15515522405082957No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC7670.1508289693878153No Hit
CCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT7650.15043567350935946No Hit
ATACTATGTTGGAGCGAGGTAATGAATCTGGCCACCTGGGTGGGAAGTAA7630.1500423776309036No Hit
GGATATTAACATTGAGAGTCCCGAGATTGAGATCTTCTGCGACGCGGCGA7510.14768260236016856No Hit
CCTTTGGAGTGTGGATTCGCACTCCTCCCGCGTACAGACCACCAAATGCC7440.14630606678557312No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC7370.14492953121097768No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC7360.14473288327174974No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG7350.14453623533252186No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT7280.1431596997579264No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG7200.141586516244103No Hit
GGGTGTACTGTTCCGGAACTGGAGCCACCAGCAGGAAGGTATAGGCCCCT7100.1396200368518238No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC7010.13785020539877252No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT7010.13785020539877252No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC7000.1376535574595446No Hit
CCGTCTGGTGGAGTGAACCCCGGCCCGAATGATCCCACTCCTATCTGGTT6850.1347038383711258No Hit
CTACCAGGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA6790.13352395073575826No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTAGGCAAGACTTGGCGGGCGTTC6750.1327373589788466No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC6720.13214741516116282No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT6660.1309675275257953No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6620.13018093576888362No Hit
GGCACTATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAA6460.12703456874123686No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG6460.12703456874123686No Hit
ATGTTGGAGCGAGGTAATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG6450.12683792080200895No Hit
TAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCT6420.12624797698432522No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6380.1254613852274135No Hit
CCTTATGAGTCCAAGGGATATTAACATTGAGAGTCCCGAGATTGAGATCT6320.12428149759204597No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6310.12408484965281807No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG6280.1234949058351343No Hit
CTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA6260.12310160995667846No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG6240.12270831407822262No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA6210.12211837026053886No Hit
AATAATGCCTGGTTTGGAAGTAATGATTAACTATATGTTCAGGATAATAA6210.12211837026053886No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6200.12192172232131093No Hit
GTCAACGAATTGTGGGTCTTTTGGGTTTTGCCGCTCCCTTTACACAATGT6140.1207418346859434No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCCTCAT6140.1207418346859434No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT6080.11956194705057589No Hit
GGATTCAGCGCTGACGGGACGTAGACAAAGGACGTCCCGCGCAGGATCCA6000.11798876353675253No Hit
GTCTAATAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGT5980.11759546765829669No Hit
GTATAAAGAATTTGGAGCTTCTCTGGAGTTACTCTCTTTTTTGCCTTCTG5940.11680887590138499No Hit
ACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGCAAACATTATCG5920.11641558002292915No Hit
GTCCAAGGGATATTAACATTGAGAGTCCCGAGATTGAGATCTTCTGCGAC5890.11582563620524539No Hit
CAGTACACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG5890.11582563620524539No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG5880.11562898826601746No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT5880.11562898826601746No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA5860.11523569238756162No Hit
CCTATCTGGTTTGCCTCTGGCCATTGATCCTTGTTGGGGTTGAAGTCCCA5840.11484239650910578No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5830.11464574856987786No Hit
TATTAACATTGACATAGTTTACTACTGATTCCCTGGATGCCGGGTCTTCC5790.11385915681296618No Hit
GCCTTAGAGTCTCCGGAGCATTGTTCACCTCACCATACAGCACTAAGGCA5770.11346586093451035No Hit
ATGTTAATATGGGCCTAAAAATGAGACAACTATTGTGGTTTCACGTTTCC5750.11307256505605448No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5700.11208932535991488No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG5680.11169602948145904No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA5670.11149938154223114No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA5660.1113027336030032No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5640.11090943772454737No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA5630.11071278978531944No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG5600.11012284596763568No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT5580.10972955008917984No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT5580.10972955008917984No Hit
TCCACAGAGTATGTAAATAATGCCTGGTTTGGAAGTAATGATTAACTATA5580.10972955008917984No Hit
GTACAAAGATCATTAACCGAGTCTCCTCCCCCAACTCCTCCCAGTCCTTA5570.10953290214995191No Hit
TATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTC5560.109336254210724No Hit
CTATTGTGGTTTCACGTTTCCTGTCTTACTTTTGGAAGAGAAACGGTTCT5540.10894295833226816No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC5530.10874631039304024No Hit
GCTAGGAGTTCCGCAGTATGGATCGGCAGAGGAGCCACAAAGGTTCCACG5510.1083530145145844No Hit
ATACAGCACTAAGGCAAGCTATACTATGTTGGAGCGAGGTAATGAATCTG5460.1073697748184448No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC5420.1065831830615331No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5380.10579659130462143No Hit
TACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGC5380.10579659130462143No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG5380.10579659130462143No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT5380.10579659130462143No Hit
ATTTAGCGCTGACGGGACGTAGACAAAGGACGTCCCGCGCAGGATCCAGT5350.10520664748693766No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5340.10500999954770973No Hit
ATTCAACGCTGACGGGACGTAGACAAAGGACGTCCCGCGCAGGATCCAGT5310.10442005573002598No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5290.10402675985157013No Hit
ACCTTTGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGA5240.10304352015543053No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG5240.10304352015543053No Hit
CCTCTAAGAGACAGTCATCCTCAGGCCATGAAATGGAATTCCAGCACATT5170.10166698458083509No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5100.10029044900623965No Hit
GCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGAT5090.10009380106701171No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)