FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508523
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG27110.5331125632468935No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC25790.5071550352688079No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA22320.43891820035671936No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT21860.429872395152235No Hit
CCTTTGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT20020.3936891743342975No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT19900.39132939906356246No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16990.33410484874823754No Hit
CCTCCCGCGTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16340.3213227326984227No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACCGAGT13000.2556423209962971No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAGGTATAGGCCCCT11870.23342110386354203No Hit
TCCGCAAACATTGTTTGATTTTTTGTACAATATGTTCCTGTGGCAAGGTA10690.2102166470346474No Hit
TACCCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG10650.20943005527773573No Hit
GTTTACTACTGATTCCCTGGATGCCGGGTCTTCCAAATTACTTCCCACCC10520.20687363206777273No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCGGCTAGG10430.20510380061472144No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG10410.20471050473626565No Hit
GTATGTAAATAATGCCTGGTTTGGAAGTAATGATTAACTATATGTTCAGG10380.20412056091858186No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10370.20392391297935392No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC10310.20274402534398642No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG10240.20136748976939098No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG10240.20136748976939098No Hit
TCTCTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG10220.2009741938909351No Hit
GTAGTAAACTATGTCAATGTTAATATGGGCCTAAAAATGAGACAACTATT10020.1970412351063767No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG9980.19625464334946502No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG9690.19055185311185532No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT9620.18917531753725988No Hit
CTACAAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA9470.18622559844884107No Hit
CTCCAAAGGACACCAAATACTCAAGAACCGTTTCTCTTCCAAAAGTAAGA9470.18622559844884107No Hit
CTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA9260.18209599172505472No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA9190.18071945615045926No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA9170.18032616027200343No Hit
GTTTTCGGAAACTTCCTGTCAATAGGCCTATTGATTGGAAAGTATGTCAA9010.17717979324435668No Hit
ACCACACGGCAATCTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGA8990.17678649736590085No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC8930.17560660973053333No Hit
ACAAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC8830.17364013033825412No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAGCATTGTTCACCTCACCATACAG8800.17305018652057036No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG8790.17285353858134245No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC8740.17187029888520283No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8700.17108370712829113No Hit
ATTCTTTGACATACTTTCCAATCAATAGGCCTATTGACAGGAAGTTTCCG8690.17088705918906325No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG8570.16852728391832816No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC8560.16833063597910025No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG8500.16715074834373272No Hit
GCACTAAGGCAAGCTATACTATGTTGGAGCGAGGTAATGAATCTGGCCAC8450.16616750864759314No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC8400.16518426895145352No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG8330.16380773337685808No Hit
GTATAGGCCCCTTACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG8220.16164460604535094No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC8100.15928483077461592No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG8030.15790829520002045No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA8000.15731835138233669No Hit
TGCAAAACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG7710.151615561144727No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA7520.1478792502993965No Hit
CCTTTGGAGTGTGGATTCGCACTCCTCCCGCGTACAGACCACCAAATGCC7410.14571612296788936No Hit
GCGCTAAATCCTGCGGACGACCCGTCCCGGGGCCGTTTGGGGCTCTACCG7380.1451261791502056No Hit
GGGTGTACTGTTCCGGAACTGGAGCCACCAGCAGGAAGGTATAGGCCCCT7360.14473288327174974No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC7350.14453623533252186No Hit
ATACTATGTTGGAGCGAGGTAATGAATCTGGCCACCTGGGTGGGAAGTAA7350.14453623533252186No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC7280.1431596997579264No Hit
ATGTAAGACCTTAGGCAAGACTTGGCGGGCGTTCACGGTGGTCTCCATGC7190.1413898683048751No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC7080.13922674097336796No Hit
GGATATTAACATTGAGAGTCCCGAGATTGAGATCTTCTGCGACGCGGCGA7070.13903009303414005No Hit
CCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT7010.13785020539877252No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6990.13745690952031667No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6980.13726026158108875No Hit
CATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACC6900.1356870780672654No Hit
GTCTAATAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGT6760.1329340069180745No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6580.12939434401197192No Hit
GTCCAAGGGATATTAACATTGAGAGTCCCGAGATTGAGATCTTCTGCGAC6530.1284111043158323No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC6530.1284111043158323No Hit
ATGTTGGAGCGAGGTAATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG6440.12664127286278104No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT6400.12585468110586934No Hit
CAGTACACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG6310.12408484965281807No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC6250.12290496201745053No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG6220.12231501819976677No Hit
CTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA6170.12133177850362717No Hit
CCTTATGAGTCCAAGGGATATTAACATTGAGAGTCCCGAGATTGAGATCT6170.12133177850362717No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG6160.12113513056439926No Hit
CTACCAGGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA6150.12093848262517133No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6110.12015189086825966No Hit
CCGTCTGGTGGAGTGAACCCCGGCCCGAATGATCCCACTCCTATCTGGTT6080.11956194705057589No Hit
GCCTTAGAGTCTCCGGAGCATTGTTCACCTCACCATACAGCACTAAGGCA6060.11916865117212003No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6020.11838205941520835No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5940.11680887590138499No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5920.11641558002292915No Hit
CCTATCTGGTTTGCCTCTGGCCATTGATCCTTGTTGGGGTTGAAGTCCCA5890.11582563620524539No Hit
GTATAAAGAATTTGGAGCTTCTCTGGAGTTACTCTCTTTTTTGCCTTCTG5890.11582563620524539No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5870.11543234032678955No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTAGGCAAGACTTGGCGGGCGTTC5850.11503904444833371No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT5820.11444910063064995No Hit
GTCAACGAATTGTGGGTCTTTTGGGTTTTGCCGCTCCCTTTACACAATGT5810.11425245269142202No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCCTCAT5800.11405580475219411No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA5790.11385915681296618No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5770.11346586093451035No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA5770.11346586093451035No Hit
GGCACTATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAA5760.11326921299528242No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG5750.11307256505605448No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC5730.11267926917759864No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA5700.11208932535991488No Hit
TATTAACATTGACATAGTTTACTACTGATTCCCTGGATGCCGGGTCTTCC5670.11149938154223114No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC5590.10992619802840776No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG5540.10894295833226816No Hit
AATAATGCCTGGTTTGGAAGTAATGATTAACTATATGTTCAGGATAATAA5510.1083530145145844No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA5510.1083530145145844No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG5500.10815636657535647No Hit
CTATTGTGGTTTCACGTTTCCTGTCTTACTTTTGGAAGAGAAACGGTTCT5490.10795971863612856No Hit
GCTAGGAGTTCCGCAGTATGGATCGGCAGAGGAGCCACAAAGGTTCCACG5480.10776307069690064No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5440.10697647893998893No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT5430.10677983100076104No Hit
TAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCT5430.10677983100076104No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT5410.1063865351223052No Hit
GGATTCAGCGCTGACGGGACGTAGACAAAGGACGTCCCGCGCAGGATCCA5400.10618988718307726No Hit
CTCCTATCTGGTTTGCCTCTGGCCATTGATCCTTGTTGGGGTTGAAGTCC5370.1055999433653935No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5370.1055999433653935No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA5310.10442005573002598No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT5280.10383011191234222No Hit
ACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGCAAACATTATCG5250.10324016809465845No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG5240.10304352015543053No Hit
GTACAAAGATCATTAACCGAGTCTCCTCCCCCAACTCCTCCCAGTCCTTA5240.10304352015543053No Hit
ATTTAGCGCTGACGGGACGTAGACAAAGGACGTCCCGCGCAGGATCCAGT5170.10166698458083509No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG5170.10166698458083509No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5120.10068374488469549No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT5120.10068374488469549No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5100.10029044900623965No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)