FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398750
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGCGAGTCCAA15150.3799373040752351No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT14030.3518495297805643No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG13450.3373040752351097No Hit
GTTATTAGACAACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG13320.3340438871473354No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT12090.3031974921630094No Hit
CCTATCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11140.2793730407523511No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC11060.2773667711598746No Hit
GTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGT10790.27059561128526644No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT10680.2678369905956113No Hit
CTATACTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT10390.260564263322884No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG9670.24250783699059558No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9260.2322257053291536No Hit
ATCCTAAACATACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT9210.2309717868338558No Hit
GCCACACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGT8930.22394984326018808No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG8190.20539184952978054No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTAAC8160.20463949843260187No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC8120.20363636363636364No Hit
GTTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG8050.20188087774294672No Hit
GCTTACTACTGCTTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC7580.19009404388714732No Hit
GTATTTTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACGAGC7430.1863322884012539No Hit
TAATATTCCTTGGACTCATAAGGTGGGAAACTTTACCGGGCTTTATTCTT7380.1850783699059561No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTGAAAATCAGACAACTATT7300.18307210031347962No Hit
GTTGTTAGACAACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG7220.18106583072100313No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC7220.18106583072100313No Hit
GGAACATTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCG7040.17655172413793102No Hit
CCTATAAATAGACCTATAGATTGGAAAGTATGTCAACGAATTGTGGGGCT6950.174294670846395No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6870.1722884012539185No Hit
GTGCTGTATGGTGAGGTGTACAATGGTGCGGAGACTCTAAGGCCTCCCGA6830.17128526645768027No Hit
CTGTGTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG6770.1697805642633229No Hit
CTCACAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCT6750.16927899686520376No Hit
GCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGT6630.16626959247648904No Hit
CTATTCTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT6580.16501567398119124No Hit
GTCACTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTGTGG6550.16426332288401255No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6180.1549843260188088No Hit
GAATATTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6160.15448275862068966No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG6090.15272727272727274No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6050.15172413793103448No Hit
GTGCTGGAGTTCCATTGCATGGCCTGAGGATGACTGTCTCTTAGAGGTGG6020.15097178683385581No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5980.14996865203761756No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5760.14445141065830722No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT5740.1439498432601881No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC5700.14294670846394983No Hit
GTTAATATTCCTTGGACTCATAAGGTGGGAAACTTTACCGGGCTTTATTC5600.1404388714733542No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5590.14018808777429467No Hit
CTCCTATCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5500.13793103448275862No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG5490.13768025078369905No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT5490.13768025078369905No Hit
AATGTATACCCAAAGATAAAAGAAAATTGGTAACAGCGGTAAAAAGGGAT5430.1361755485893417No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA5180.12990595611285266No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5150.129153605015674No Hit
CTTTTGGAGTGTGGATTCGCACTCCTATCGCTTACAGACCACCAAATGCC5060.1268965517241379No Hit
GTATCCATTTAAACCCTACTAAAACTAAACGTTGGGGCTACAACCTTAAC5030.12614420062695925No Hit
GTCTAATAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGT5030.12614420062695925No Hit
ATATAAGAGAGAAACTACTCGAAGCGCCTCATTTTGTGGGTCACCATATT5010.12564263322884012No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4930.12363636363636364No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4930.12363636363636364No Hit
AGGTAGGAGTGGGATCCTTCGGGCCGGGGTTCACTCCACCACACGGCAAT4930.12363636363636364No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4920.12338557993730406No Hit
ATACTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA4890.1226332288401254No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA4880.12238244514106583No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4860.1218808777429467No Hit
GTTAATCATTACTTCCAAACTAGGCATTATTTACATACTCTGTGGAAGGC4780.11987460815047021No Hit
ACGTTGGGGCTACAACCTTAACTTCATGGGATATGTAATTGGAAGTTGGG4740.11887147335423198No Hit
ATAGTATAGCTTGCCTTAGTGCGGTATGGTGAGGTGTACAATGGTGCGGA4720.11836990595611285No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG4720.11836990595611285No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG4700.11786833855799374No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG4620.11586206896551723No Hit
TCCTCAGGCCATGCAATGGAACTCCAGCACATTCCACCAAGCTCTGCTAG4600.11536050156739812No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA4580.11485893416927899No Hit
CTATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT4560.11435736677115989No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4560.11435736677115989No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4530.1136050156739812No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG4520.11335423197492162No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4520.11335423197492162No Hit
ATTTTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACGAGCAG4440.11134796238244514No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC4400.1103448275862069No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT4400.1103448275862069No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGAAGCGCCTCAT4330.10858934169278997No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT4310.10808777429467085No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC4290.10758620689655173No Hit
ATAATATCCTCTTTCAAATGAATGTTAGGAAAAGAGGGAGTTTGCCAGTC4280.10733542319749216No Hit
GACCTATAGATTGGAAAGTATGTCAACGAATTGTGGGGCTTCTGGGCTTT4270.1070846394984326No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4260.10683385579937305No Hit
ATACTCTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4230.10608150470219435No Hit
GTTAATATGGGCCTGAAAATCAGACAACTATTGTGGTTTCACATTTCCTG4210.10557993730407525No Hit
ATACCGCACTAAGGCAAGCTATACTATGTTGGGGTGAGTTGATGAATCTG4200.10532915360501567No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4170.10457680250783698No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTAACATTCATTTGAAAG4170.10457680250783698No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGAAGCGCC4160.10432601880877744No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCGGTAAAGTTTCCCAC4150.10407523510971788No Hit
GTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACGAGC4140.10382445141065831No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4110.10307210031347963No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT4110.10307210031347963No Hit
CCACCACATTCCACCAAGCTCTGCTAGATCCCAGAGTGAGGGGCCTATAC4110.10307210031347963No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4070.10206896551724137No Hit
GTGCGGTATGGTGAGGTGTACAATGGTGCGGAGACTCTAAGGCCTCCCGA4060.10181818181818182No Hit
GTACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTA4050.10156739811912226No Hit
TCTCATTGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTG4010.10056426332288401No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA4010.10056426332288401No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG3990.10006269592476488No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)