FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398750
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT13990.350846394984326No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGCGAGTCCAA13720.3440752351097179No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG13290.33329153605015677No Hit
GTTATTAGACAACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG12120.3039498432601881No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC11950.29968652037617555No Hit
GTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGT11360.28489028213166145No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT11110.2786206896551724No Hit
CCTATCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10850.2721003134796238No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10480.2628213166144201No Hit
CTATACTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT9650.2420062695924765No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG9350.23448275862068968No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT8860.22219435736677115No Hit
GTTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG8580.21517241379310345No Hit
GTATTTTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACGAGC8300.20815047021943575No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG7910.19836990595611284No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTAAC7530.18884012539184952No Hit
ATCCTAAACATACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT7410.1858307210031348No Hit
GCCACACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGT7190.18031347962382444No Hit
TAATATTCCTTGGACTCATAAGGTGGGAAACTTTACCGGGCTTTATTCTT7070.17730407523510971No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6970.17479623824451412No Hit
GCTTACTACTGCTTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC6930.17379310344827587No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTGAAAATCAGACAACTATT6930.17379310344827587No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC6900.17304075235109717No Hit
GGAACATTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCG6720.1685266457680251No Hit
GTGCTGTATGGTGAGGTGTACAATGGTGCGGAGACTCTAAGGCCTCCCGA6610.1657680250783699No Hit
GTTGTTAGACAACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG6610.1657680250783699No Hit
CTCACAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCT6410.16075235109717867No Hit
CTATTCTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGT6350.1592476489028213No Hit
GTCACTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTGTGG6310.15824451410658308No Hit
CCTATAAATAGACCTATAGATTGGAAAGTATGTCAACGAATTGTGGGGCT6280.15749216300940438No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6180.1549843260188088No Hit
CTCCTATCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG6120.1534796238244514No Hit
GTGCTGGAGTTCCATTGCATGGCCTGAGGATGACTGTCTCTTAGAGGTGG6040.15147335423197494No Hit
CTGTGTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG6030.15122257053291538No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC5990.15021943573667712No Hit
GAATATTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5930.14871473354231976No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG5910.14821316614420063No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5900.14796238244514107No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5780.14495297805642635No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC5730.14369905956112852No Hit
GTCTAATAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGT5700.14294670846394983No Hit
GTATCCATTTAAACCCTACTAAAACTAAACGTTGGGGCTACAACCTTAAC5500.13793103448275862No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5470.13717868338557992No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT5440.13642633228840126No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT5380.1349216300940439No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5360.13442006269592477No Hit
GCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGT5350.1341692789968652No Hit
CTTTTGGAGTGTGGATTCGCACTCCTATCGCTTACAGACCACCAAATGCC5340.13391849529780564No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5330.13366771159874608No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG5280.13241379310344828No Hit
GTTAATATTCCTTGGACTCATAAGGTGGGAAACTTTACCGGGCTTTATTC5240.13141065830721005No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA5040.1263949843260188No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5020.12589341692789968No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5000.12539184952978058No Hit
AATGTATACCCAAAGATAAAAGAAAATTGGTAACAGCGGTAAAAAGGGAT4960.12438871473354231No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4880.12238244514106583No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4710.1181191222570533No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4690.11761755485893417No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA4640.11636363636363636No Hit
ACGTTGGGGCTACAACCTTAACTTCATGGGATATGTAATTGGAAGTTGGG4630.1161128526645768No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4610.1156112852664577No Hit
ATACTCTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4600.11536050156739812No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAGAGTATAGGCCCCT4580.11485893416927899No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA4560.11435736677115989No Hit
AGGTAGGAGTGGGATCCTTCGGGCCGGGGTTCACTCCACCACACGGCAAT4530.1136050156739812No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4520.11335423197492162No Hit
GTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACGAGC4500.11285266457680251No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4470.11210031347962382No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4450.1115987460815047No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4450.1115987460815047No Hit
CTATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT4450.1115987460815047No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4410.11059561128526646No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4380.10984326018808777No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC4350.1090909090909091No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG4350.1090909090909091No Hit
ATACTATGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA4340.10884012539184953No Hit
ATAATATCCTCTTTCAAATGAATGTTAGGAAAAGAGGGAGTTTGCCAGTC4310.10808777429467085No Hit
TCCTCAGGCCATGCAATGGAACTCCAGCACATTCCACCAAGCTCTGCTAG4260.10683385579937305No Hit
CTCCACCACATTCCACCAAGCTCTGCTAGATCCCAGAGTGAGGGGCCTAT4250.10658307210031348No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTAACATTCATTTGAAAG4230.10608150470219435No Hit
ATATAAGAGAGAAACTACTCGAAGCGCCTCATTTTGTGGGTCACCATATT4160.10432601880877744No Hit
GTTAATATGGGCCTGAAAATCAGACAACTATTGTGGTTTCACATTTCCTG4150.10407523510971788No Hit
ATTTTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACGAGCAG4140.10382445141065831No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4110.10307210031347963No Hit
GACCTATAGATTGGAAAGTATGTCAACGAATTGTGGGGCTTCTGGGCTTT4100.10282131661442007No Hit
ATAGTATAGCTTGCCTTAGTGCGGTATGGTGAGGTGTACAATGGTGCGGA4090.1025705329153605No Hit
CTCCCACCTGGTTTGACGCTGGCCATTGATCCTTGTTGGGGTTGAAGTCC4080.10231974921630094No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT4080.10231974921630094No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG4010.10056426332288401No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG4010.10056426332288401No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCGGTAAAGTTTCCCAC4000.10031347962382445No Hit
TCTCATTGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTG4000.10031347962382445No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)