FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466283
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT23480.5035568528125622No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA22810.4891878966207217No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT18030.3866750449834114No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC15370.32962814428147713No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT13530.29016713026209406No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG13100.2809452628553904No Hit
CTCCTAACGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG12580.2697932371542604No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTGAAAATCAGACAACTATT12450.2670052307289779No Hit
CCTAACGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10840.23247684346201772No Hit
CCTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT10240.21960912149917539No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA9790.20995833002704364No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC9760.20931494392890157No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG9640.2067413995363331No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG9600.20588355140547693No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG9590.2056690893727629No Hit
GCTTACTACTGCTTCCCTGGATGCCGGGTCGTCCAAATTACTTCCCACCC9180.19687614603148734No Hit
GATTTATATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAA9060.19430260163891885No Hit
CTACTGCACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC9030.19365921554077675No Hit
GGAACATATTGTGCAAAAAATCAAACAATGTTTTCGGAAACTTCCTACAA9010.19323029147534865No Hit
GGTTAGGATAGAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCG8870.1902278230173521No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG8730.1872253545593556No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAC8700.18658196846121347No Hit
GTCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC8540.18315057593778886No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC8430.18079149357793442No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8290.17778902511993788No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG8250.17693117698908173No Hit
ATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC8230.17650225292365365No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG8200.17585886682551155No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8110.17392870853108522No Hit
CTTTTGGAGTGTGGATTCGCACTCCTAACGCTTACAGACCACCAAATGCC8010.1717840882039448No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTAATCATGCCTGCTAGG7990.17135516413851673No Hit
CATATAAATCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7980.1711407021058027No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT7860.16856715771323424No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG7560.1621332967318131No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7550.16191883469909907No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG7550.16191883469909907No Hit
GTTCAGGATAGTAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA7220.1548415876195358No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAATCGAAGTGCACACGGTCCG7150.1533403533905375No Hit
GCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGG7120.1526969672923954No Hit
CATTTAAATCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7120.1526969672923954No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAG6970.1494800368016848No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC6940.14883665070354268No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6930.14862218867082866No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6920.1484077266381146No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6800.14583418224554615No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA6780.14540525818011807No Hit
CCCCAAAAGATTGCCGTGTGGTGGAGTGAACCCTGGCCCGAATGATCCCA6740.14454741004926192No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6690.14347509988569174No Hit
GTATTCATTTAAATCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC6640.14240278972212156No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6640.14240278972212156No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT6640.14240278972212156No Hit
GGGTAAAGGTGCAAATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6620.14197386565669348No Hit
TAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCT6510.13961478329683905No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG6380.13682677687155653No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6380.13682677687155653No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6370.1366123148388425No Hit
ATCCTGAACATATAGTTAATCATTACTTCCAAACTAGGCATTATTTACAT6320.1355400046752723No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6240.13382430841356No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6230.13360984638084597No Hit
GCATATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGG6090.13060737792284943No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6090.13060737792284943No Hit
TACCTTGGCAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6080.1303929158901354No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT6060.12996399182470733No Hit
TTATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATA6050.12974952979199328No Hit
CAATAGGCCTATTTGTAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGC6000.1286772196284231No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC5950.1276049094648529No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCATTC5950.1276049094648529No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5930.12717598539942482No Hit
GTTAATATGGGCCTGAAAATCAGACAACTATTGTGGTTTCACATTTCCTG5890.12631813726856866No Hit
GTGCAGAGGTGAATCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5870.12588921320314056No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGAGCATGGACATTGA5810.12460244100685636No Hit
GGATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5810.12460244100685636No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5770.1237445928760002No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC5770.1237445928760002No Hit
TACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGC5690.1220288966142879No Hit
ACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAGGAACAT5660.12138551051614577No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5640.12095658645071769No Hit
ATGTTAATATGGGCCTGAAAATCAGACAACTATTGTGGTTTCACATTTCC5590.11988427628714751No Hit
GTCATTATGCCTTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTGTGG5580.11966981425443346No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACGG5540.1188119661235773No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCACCCAAG5500.11795411799272117No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG5440.11666734579643694No Hit
GTCAACATGCCTTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTGTGG5360.11495164953472463No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCCTCAT5340.11452272546929655No Hit
TCCTCAGGCCATGCAGTGGACTGCTGGTGGCTCCAGTTCCGGAACAGTAA5340.11452272546929655No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG5340.11452272546929655No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTAGGCAAGACCTGGCGGGCGTTC5310.11387933937115442No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5270.11302149124029827No Hit
GCCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACCACC5260.11280702920758423No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA5230.11216364310944213No Hit
ATATGGGAGAGGCAGTAGTCGGAACAGGGTTTACTGTTCCGGAACTGGAG5210.11173471904401405No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGTCCACTGCATGGCCTG5200.1115202570113No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC5180.11109133294587192No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG5160.11066240888044385No Hit
GTCAATATGCCTTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTGTGG5160.11066240888044385No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA5150.11044794684772982No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAGCACTAAGGCA5140.11023348481501577No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC5140.11023348481501577No Hit
CCCAAGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACC5100.10937563668415962No Hit
GATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCATT5060.10851778855330346No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC5040.10808886448787539No Hit
GACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACAGCCGAGCAGCC5030.10787440245516135No Hit
GGTCTTGCATGGTCCCGTGCTGGTGGTTGATGTTCCTGGAAGTAGAGGAC5000.10723101635701923No Hit
CTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCGTGATCTCCTCGACACC4990.1070165543243052No Hit
TGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4970.10658763025887712No Hit
CCTTATGCGTCCAAGGGATACTAACATTGAGGTTCCCGAGATTGAGATCT4930.10572978212802096No Hit
GCCTAATCATCTCTTGTTCATGTTCCACTGTTCAAGCCTCCAAGCTGTGC4930.10572978212802096No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4890.10487193399716481No Hit
GTGTAAACAATATTTGCACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC4880.10465747196445079No Hit
CCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGGGA4870.10444300993173673No Hit
GGATAGAACCTAGCAGGCATGATTAATTTTAATCTCCTTTTTTCGTTAAC4870.10444300993173673No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4850.10401408586630866No Hit
TATTAACATTGACATAGCTTACTACTGCTTCCCTGGATGCCGGGTCGTCC4780.10251285163731039No Hit
GAATAAAGTCCAGTAAAGTTTCCCACCTTATGCGTCCAAGGGATACTAAC4720.10122607944102616No Hit
ATTTATGCCTACAGCCTCCCAGTACAAAGATCATTAACCTAGTCTCCTCC4710.1010116174083121No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4680.10036823131017No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)