FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences627603
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC26780.4267028678957876No Hit
GCCTGCGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT25170.4010497081753911No Hit
TCCTCATGCTATGCAGTGGAACTCCAGCACGTTCCACCAAGCTCTGCTAG24340.3878247873257457No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC22360.3562761809615314No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT20390.32488691099309597No Hit
TTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAATGGCAA20090.32010681911973016No Hit
GGAAAGTATAGGCCCCTCACCCTGGGATCTAGCAGAGCTTGGTGGAACGT19210.30608521629119045No Hit
CATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTCTT17910.2853714848399386No Hit
CCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC17580.2801133837792362No Hit
ACCCATCTCGGGGCCGTTTGGGACTCTACCGTCCCCTTCTGCGTCTGCCG17210.27421793713541837No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT17170.27358059155230297No Hit
ATACCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA15650.2493614593939162No Hit
GTTGCACAGTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCC13580.21637882546769216No Hit
ATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAA13310.21207674278166294No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12960.2064999689294028No Hit
ATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA12900.20554395055472968No Hit
ACAATACCAGCTGCGCCTCCTCCTGCCTCCACCAATCGGCAGTCAGGAAG12370.19709912157845005No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12360.1969397851826712No Hit
ATCCTAACCGTACCAAATATTTGCCATTAGATAAAGGCATTAAACCTTAT12050.19200035691352654No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCACCCACG11800.18801694701905505No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG11720.18674225585282417No Hit
CCCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC11370.18116548200056404No Hit
TTGTTCACATCTCTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAG11120.17718207210609255No Hit
GAAGATGAGGCATAGCAGCAGGATGAAGAGGAAGATGATAAAACGCCGCA10950.17447335337785191No Hit
ATATAGGGCTGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAAGAAGT10840.17272065302428447No Hit
GTTTCTCTTCCAAAAGTGAGACAGGAAATGTGAAACCACAATATTTGTCT10750.17128662546227472No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC10670.17001193429604383No Hit
ACACTTGGGTGTTTCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA10660.16985259790026497No Hit
GAAGACAAACTGTTCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGC10360.16507250602689916No Hit
CCAATAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCAC10250.1633198056733317No Hit
GGGGTCCTAGGAATCCTGATGTGGTGCTCTCCATATTCGGTGCAGGGTCC9840.15678701344639845No Hit
ATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAA9770.15567165867594643No Hit
ATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAATGGCA9680.15423763111393668No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9430.1502542212194652No Hit
CTGATGGCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACA9300.14818284807433998No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8990.14324341980519534No Hit
TTAATTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAATGGCAA8950.14260607422207988No Hit
GTATAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC8950.14260607422207988No Hit
GGCCACCAGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGATCCATAC8910.14196872863896445No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC8800.14021602828539698No Hit
GGTTTGCCGCTGGCCATTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA8690.13846332793182953No Hit
GCGTACTACTGCTTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC8650.13782598234871407No Hit
GTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGGTTCTATCC8520.1357546092035889No Hit
GTTGACATCTATTGAGAATATCCTCTTTCAAATGAATGTGAGGAAAAGAG8450.13463925443313687No Hit
GCCCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCATCTCACCA8440.134479918037358No Hit
GAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGGACGTAGACAAAGGAC8290.1320898721006751No Hit
GTAGTACGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAAATATT8120.1293811533724345No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG8010.12762845301886702No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAATTATGTGTTCAGG7990.1273097802273093No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG7920.1261944254568573No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT7630.12157366997927034No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG7590.12093632439615488No Hit
GTCCTCGAGAAGATTGACGATATGGGAGAGGCAGTAGTCGGAACAGGGTT7590.12093632439615488No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAATGGCAAATATTTGGTA7570.12061765160459718No Hit
ACACGTGGGTGCTTCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA7530.11998030602148173No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7490.11934296043836629No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7420.11822760566791427No Hit
CATAATTAATCATTACTTCCAAACTAGGCATTATTTACATACTCTGTGGA7240.1153595505438948No Hit
CTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTC7210.1148815413565582No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7150.11392552298188505No Hit
GTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG7130.11360685019032733No Hit
GTTGTTCAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCC7110.1132881773987696No Hit
TCCGCAGGCTATGCAGTGGAACTCCAGCACGTTCCACCAAGCTCTGCTAG7100.11312884100299074No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTAGTAACAGCGGTAAAAA7090.11296950460721188No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7020.11185414983675987No Hit
GCCTTAGAGTCTCCGGAACATTGTTCATCTCACCATACAGCACTACGGCA7020.11185414983675987No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6990.11137614064942328No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6970.11105746785786555No Hit
ATACAGCACTACGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG6960.11089813146208671No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCATTAGATAAAGGCATTAAAC6960.11089813146208671No Hit
GATGTGAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTA6900.10994211308741354No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6800.10834874912962493No Hit
GGACCCTGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCC6750.10755206715073062No Hit
CATAGGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTCTT6560.1045246756309323No Hit
CTCTAAGGCCTCCCGATATAGGGCTGAGGCGGTGTCGAGGAGATCACGAA6520.10388733004781685No Hit
GGTCCTAGGAATCCTGATGTGGTGCTCTCCATATTCGGTGCAGGGTCCCC6500.10356865725625913No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT6490.10340932086048027No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG6430.10245330248580711No Hit
CATTTAAACACTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT6300.10038192934068192No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)