FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences627603
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC27780.44263650747367367No Hit
GCCTGCGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT24160.38495673220172627No Hit
TCCTCATGCTATGCAGTGGAACTCCAGCACGTTCCACCAAGCTCTGCTAG22560.35946290887710863No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC21750.3465566608190209No Hit
TTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAATGGCAA20020.3189914643492781No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT19900.3170794275999318No Hit
ACCCATCTCGGGGCCGTTTGGGACTCTACCGTCCCCTTCTGCGTCTGCCG19620.3126180085181237No Hit
GGAAAGTATAGGCCCCTCACCCTGGGATCTAGCAGAGCTTGGTGGAACGT18750.2987557420853629No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT16180.2578062883701958No Hit
CATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTCTT15900.25334486928838773No Hit
ATACCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA15560.24792743183190646No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13580.21637882546769216No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12910.20570328695050855No Hit
ATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA12680.20203854984759476No Hit
GTTGCACAGTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCC12480.19885182193201753No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCACCCACG12120.19311571168397856No Hit
ATCCTAACCGTACCAAATATTTGCCATTAGATAAAGGCATTAAACCTTAT12080.19247836610086314No Hit
ATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAA11900.18961031097684364No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG11780.18769827422749732No Hit
GTTTCTCTTCCAAAAGTGAGACAGGAAATGTGAAACCACAATATTTGTCT11630.18530822829081442No Hit
GAAGATGAGGCATAGCAGCAGGATGAAGAGGAAGATGATAAAACGCCGCA11410.1818028275836795No Hit
CCCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC11370.18116548200056404No Hit
ACAATACCAGCTGCGCCTCCTCCTGCCTCCACCAATCGGCAGTCAGGAAG11290.17989079083433318No Hit
TTGTTCACATCTCTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAG11130.1773414085018714No Hit
ATATAGGGCTGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAAGAAGT10260.16347914206911057No Hit
GAAGACAAACTGTTCTTGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGC9940.15838037740418703No Hit
ACACTTGGGTGTTTCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA9750.1553529858843887No Hit
CTGATGGCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACA9550.1521662579688115No Hit
GGGGTCCTAGGAATCCTGATGTGGTGCTCTCCATATTCGGTGCAGGGTCC9510.15152891238569605No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC9430.1502542212194652No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9380.14945753924057087No Hit
GTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGGTTCTATCC9220.1469081569081091No Hit
GGTTTGCCGCTGGCCATTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA9190.14643014772077254No Hit
TTAATTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAATGGCAA9150.14579280213765708No Hit
GAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGGACGTAGACAAAGGAC8780.13989735549383925No Hit
ATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAATGGCA8760.13957868270228155No Hit
CCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC8730.13910067351494496No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAATTATGTGTTCAGG8710.13878200072338726No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC8630.13750730955715634No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8590.13686996397404091No Hit
GGCCACCAGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGATCCATAC8520.1357546092035889No Hit
GTATAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC8480.13511726362047347No Hit
ATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAA8480.13511726362047347No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG8260.13161186291333854No Hit
GCCCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCATCTCACCA8260.13161186291333854No Hit
CCAATAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCAC8080.12874380778931904No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG8000.12746911662308816No Hit
GTCCTCGAGAAGATTGACGATATGGGAGAGGCAGTAGTCGGAACAGGGTT7990.1273097802273093No Hit
GCGTACTACTGCTTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC7950.12667243464419387No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCATTAGATAAAGGCATTAAAC7920.1261944254568573No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG7810.12444172510328982No Hit
GTAGTACGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAAATATT7750.12348570672861667No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTAGTAACAGCGGTAAAAA7560.12045831520881832No Hit
GTCTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG7510.11966163322992401No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAATGGCAAATATTTGGTA7480.11918362404258742No Hit
GTTGACATCTATTGAGAATATCCTCTTTCAAATGAATGTGAGGAAAAGAG7370.11743092368901996No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7280.11599689612701022No Hit
GAAGTTTTCGAAAACATTGTTTGATTTTGTTTACAATATGTTCCTGTGGT7170.11424419577344277No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT7110.1132881773987696No Hit
CTCTAAGGCCTCCCGATATAGGGCTGAGGCGGTGTCGAGGAGATCACGAA6990.11137614064942328No Hit
GTATAGGCCCCTCACCCTGGGATCTAGCAGAGCTTGGTGGAACGTGCTGG6980.11121680425364441No Hit
GTTGTTCAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCC6850.10914543110851925No Hit
GGACCCTGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCC6820.10866742192118266No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6800.10834874912962493No Hit
TCCGCAGGCTATGCAGTGGAACTCCAGCACGTTCCACCAAGCTCTGCTAG6780.10803007633806722No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT6730.10723339435917292No Hit
ATACAGCACTACGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG6690.10659604877605747No Hit
CATAATTAATCATTACTTCCAAACTAGGCATTATTTACATACTCTGTGGA6680.10643671238027863No Hit
ACACGTGGGTGCTTCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTA6670.10627737598449977No Hit
GCCTTAGAGTCTCCGGAACATTGTTCATCTCACCATACAGCACTACGGCA6640.10579936679716317No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6520.10388733004781685No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6510.103727993652038No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6460.10293131167314368No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6430.10245330248580711No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6420.10229396609002825No Hit
GGATAATAAGGTTTAATGCCTTTATCTAATGGCAAATATTTGGTACGGTT6370.10149728411113394No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6360.10133794771535508No Hit
CTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTC6330.10085993852801851No Hit
GATGTGAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTA6320.10070060213223965No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)