FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417793
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC19490.4664989600112974No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA18480.4423243089281055No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG17940.4293992479529336No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT17220.41216583331937107No Hit
TATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTCC16310.3903847120463962No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA16000.3829647696347234No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA15090.36118364836174854No Hit
GTTGTAAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG14770.35352435296905405No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG14170.33916317410775193No Hit
ACGTCAATATGGGCTTAAAAATCAGACAACTATTGTGGTTTCACATTTCC14000.335094173430383No Hit
ACTTACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT13690.32767423101871024No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT13590.3252807012084932No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGA13350.3195362296639723No Hit
GTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTTCCTGTGGCAAT12210.2922499898274983No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA11240.2690327506683932No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA10840.2594586314275251No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGT10780.2580225135413949No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC9330.2233163312932481No Hit
AAGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT9150.21900797763485746No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG9020.2158963888815753No Hit
GTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGGCCCTGACTCTGGG9000.2154176829195319No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA8670.20751903454581574No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT8400.2010565040582298No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC8340.1996203861720996No Hit
GATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCAGTCTTCGATAAGAT8320.19914168021005618No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA8020.1919610907794051No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7470.1787966768232115No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT7420.17759991191810298No Hit
GCATCAAGGCAGGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAA7180.17185544037358214No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG7170.17161608739256043No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7100.16994061652540854No Hit
CTCTTTGGAAGGCGGGTATATTATATAAAAGAGAGTCCACACGTAGCGCC7090.16970126354438683No Hit
CACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCGG7060.1689832046013217No Hit
TTTAAAAAACTTCCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACG6880.16467485094293108No Hit
CTTTTATATAATATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT6750.16156326218964895No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6660.15940908536045362No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6660.15940908536045362No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGA6580.15749426151228002No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6540.1565368495881932No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6410.15342526083491106No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG6410.15342526083491106No Hit
CAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTAAGTAAA6380.15270720189184597No Hit
CCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAG6340.15174978996775915No Hit
ACACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCG6330.15151043698673747No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6300.15079237804367235No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT6240.14935626015754214No Hit
CAAGAAGGGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTAAGTAAA6150.1472020833283468No Hit
GTTCAGAAACAGTGAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCA5910.14145761178382596No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA5880.14073955284076087No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA5850.14002149389769575No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5820.13930343495463066No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5810.13906408197360895No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC5630.1347557283152183No Hit
ATTGTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA5600.1340376693721532No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG5490.13140478658091448No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA5450.13044737465682765No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAGAAGGGCTTTTACTTT5440.13020802167580595No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA5430.12996866869478427No Hit
ATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA5420.12972931571376256No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5400.12925060975171915No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5400.12925060975171915No Hit
CCCCAACAGTCCCCCATGGGGCGGAATGAACCCTGGCCCGAATGCTCCCA5300.12685707994150214No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5300.12685707994150214No Hit
CTGTAAGGGGCCCCACAAATTGCTTGCATCTATCAACAATGTCCTCCTGC5180.1239848441692417No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5110.12230937330208978No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5100.12207002032106809No Hit
GGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATGATTAATTT5090.12183066734004638No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT5070.12135196137800297No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA4990.11943713752982936No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG4990.11943713752982936No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC4950.11847972560574255No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4940.11824037262472084No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4910.11752231368165574No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG4880.11680425473859063No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG4840.11584684281450383No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATATCCACATTGCGTGAAA4820.11536813685246042No Hit
CCTGTTAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4810.11512878387143873No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4810.11512878387143873No Hit
GTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGATAATAAGGTTTG4720.1129746070422434No Hit
TTAAAAAACTTCCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGA4710.1127352540612217No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG4680.1120171951181566No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4670.11177784213713489No Hit
ATATCTGCCCTTAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAG4650.11129913617509149No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT4630.11082043021304808No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4630.11082043021304808No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT4590.10986301828896126No Hit
CATCTCATCAGGACTCCTAGGACCCCTGCTCGTGTTACAGGCGGGGGTTT4530.10842690040283105No Hit
ATATTGACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC4510.10794819444078765No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT4490.10746948847874425No Hit
GGCCCATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGG4470.10699078251670084No Hit
GGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAG4390.10507595866852724No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC4340.10387919376341874No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC4340.10387919376341874No Hit
GAATAAAGTCCCGTAAAGTTTCCCACCTTATGTGTCCAAGGAATACTAAC4300.10292178183933193No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGACTTTATTCTTCTACGG4300.10292178183933193No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC4290.10268242885831022No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA4290.10268242885831022No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA4220.10100695799115832No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG4190.10028889904809321No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)