FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417793
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA18500.44280301489014895No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC17400.41647418697776173No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG16940.4054639498507634No Hit
TATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTCC16670.39900141936317746No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT16590.39708659551500386No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA14880.35615723576029273No Hit
GTTGTAAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG14620.34993405825372853No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT13190.31570658196762513No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT13150.3147491700435383No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG13130.3142704640814949No Hit
ACTTACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT12620.3020634620493881No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA12600.3015847560873447No Hit
ACGTCAATATGGGCTTAAAAATCAGACAACTATTGTGGTTTCACATTTCC12260.29344675473260684No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA12140.2905745189603464No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGA12120.290095812998303No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA11120.2661605148961328No Hit
GTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTTCCTGTGGCAAT10570.2529961009399392No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGT10290.2462942174713315No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG8790.21039127031807617No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC8170.19555138549473064No Hit
GTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGGCCCTGACTCTGGG8130.19459397357064384No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA8080.1933972086655353No Hit
AAGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT8000.1914823848173617No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7960.19052497289327489No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT7940.1900462669312315No Hit
CTTTTATATAATATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT7610.18214761855751532No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA7450.17831797086116807No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG7430.1778392648991247No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7410.17736055893708128No Hit
GATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCAGTCTTCGATAAGAT7330.17544573508890765No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC7050.1687438516203No Hit
CTCTTTGGAAGGCGGGTATATTATATAAAAGAGAGTCCACACGTAGCGCC7010.1677864396962132No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT6700.16036649728454044No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG6690.16012714430351874No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6590.1577336144933017No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6390.15294655487286768No Hit
CACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCGG6310.15103173102469405No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG6230.14911690717652043No Hit
GCATCAAGGCAGGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAA6230.14911690717652043No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGA6190.14815949525243363No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC6190.14815949525243363No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA6160.1474414363093685No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6100.1460053184232383No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6080.1455266124611949No Hit
CAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTAAGTAAA6010.143851141594043No Hit
ATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA5990.14337243563199958No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5940.14217567072689108No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5850.14002149389769575No Hit
TAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATTGGG5850.14002149389769575No Hit
CAAGAAGGGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTAAGTAAA5740.13738861110645703No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5660.1354737872582834No Hit
GTTCAGAAACAGTGAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCA5650.1352344342772617No Hit
TTTAAAAAACTTCCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACG5570.13331961042908808No Hit
CTGTAAGGGGCCCCACAAATTGCTTGCATCTATCAACAATGTCCTCCTGC5490.13140478658091448No Hit
ACACACAATAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCG5490.13140478658091448No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5490.13140478658091448No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA5480.13116543359989277No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5430.12996866869478427No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAGAAGGGCTTTTACTTT5400.12925060975171915No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5260.1258996680174153No Hit
ATATTGACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC5230.12518160907435022No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5170.12374549118822No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5160.1235061382071983No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA5120.12254872628311149No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG5040.12063390243493786No Hit
ATTGTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA4990.11943713752982936No Hit
GGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATGATTAATTT4990.11943713752982936No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA4930.11800101964369915No Hit
GTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGATAATAAGGTTTG4750.1136926659853085No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4750.1136926659853085No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCAC4700.1124959010802No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACAAGACATT4700.1124959010802No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT4680.1120171951181566No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC4670.11177784213713489No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATATCCACATTGCGTGAAA4670.11177784213713489No Hit
GGCCCATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGG4610.11034172425100468No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA4590.10986301828896126No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4580.10962366530793957No Hit
CCCCAACAGTCCCCCATGGGGCGGAATGAACCCTGGCCCGAATGCTCCCA4510.10794819444078765No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4510.10794819444078765No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC4450.10651207655465744No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG4450.10651207655465744No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG4440.10627272357363574No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG4440.10627272357363574No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT4420.10579401761159234No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG4410.10555466463057063No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4390.10507595866852724No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4360.10435789972546214No Hit
GAATAAAGTCCCGTAAAGTTTCCCACCTTATGTGTCCAAGGAATACTAAC4300.10292178183933193No Hit
CTGCCTCTTCGTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGG4290.10268242885831022No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4290.10268242885831022No Hit
TTAAAAAACTTCCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGA4260.10196436991524511No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)