FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002489450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666313
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44670.6704056501974297No Hit
GTATGTAAATAATGCCTAGTTTGAAAGTAATGATTAACTATGTGTTCAGG19940.2992587567704667No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT17810.26729179829899763No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG16310.244779855713456No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATG16150.2423785818376649No Hit
ATAGTAAACTATGTCAATGTTAATATGGGCATAAAAATCAGACAATTATT14710.2207671169555449No Hit
CTCAAGAACAGTATCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA14180.21281289724198685No Hit
CCCTTTACACAATGTGGTTACCCAGCTTTAATGCCTTTGTATGCATGTAT13200.19810509475276633No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTATCTCTTCCAAAAGTAAGA13140.1972046170493447No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT12640.18970063618749747No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA12400.1860987253738108No Hit
ACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGA12270.18414769034973053No Hit
ATGTTAATATGGGCATAAAAATCAGACAATTATTGTGGTTTCACATTTCC12160.18249681456012415No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC11700.1755931521672247No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11700.1755931521672247No Hit
GTTAATATGGGCATAAAAATCAGACAATTATTGTGGTTTCACATTTCCTG11260.16898964900879918No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT11010.16523765857787556No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA10370.15563256307471113No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATG10200.1530812095816831No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA9900.14857882106457476No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC9880.14827866183010088No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC9880.14827866183010088No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9860.14797850259562698No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG9560.14347611407851865No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8880.13327070010640646No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGAAAGTAATGATTAACTATG8740.13116958546508922No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC8610.12921855044100897No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGTGAGG8550.1283180727375873No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGA8450.12681727656521785No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC8420.12636703771350702No Hit
GTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAA8310.12471616192390063No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCGTAT8170.1226150472825834No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAA8070.12111425111021397No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT7900.11856289761718593No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT7730.11601154412415786No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT7650.1148109071862623No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG7650.1148109071862623No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC7610.11421058871731453No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7560.11346019063112982No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7500.11255971292770815No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7460.11195939445876038No Hit
GGATAATACGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT7440.11165923522428649No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC7300.10955812058296927No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG7280.10925796134849539No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA7190.10790724479336287No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7190.10790724479336287No Hit
ATATATGACCCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAG7160.10745700594165204No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7150.10730692632441509No Hit
GGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT7070.10610628938651955No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTC6980.10475557283138705No Hit
GGAACATATTGTACACAAAATCAAACAATGTTTTCGGAAACTTCCTGTAA6920.10385509512796538No Hit
CAATAGGTCTATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT6920.10385509512796538No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6820.10235429895559595No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6790.10190406010388511No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGGTT6780.10175398048664816No Hit
TATTAACATTGACATAGTTTACTATTAATTCCCTGGATGCTGGGTCTTCC6710.10070342316598957No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)