FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428094
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21680.5064308306119685No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT15810.36931141291398617No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA11920.2784435194139605No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10850.25344900886253957No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC10620.2480763570617668No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCGTTTTGTTTTG10550.24644120216587945No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCACTCGGCAGT9700.2265857498586759No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9400.21957794316201582No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATT8110.18944437436637748No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA8040.18780921947049015No Hit
CATTTAAACCCTCACAAAACAAAACGATGGGGATATTCCCTTAACTTCAT7430.17356001252061465No Hit
GGATAATACGGTTTGATCCCTTTATCCAAAGGGAAATATTTAGTAACATT7400.17285923185094862No Hit
ACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTC7250.16935532850261859No Hit
GGGCTCTACCGCCCGCTTCTCCGCCTGTTGTACCGACCGACCACGGGGCG6840.1597779926838498No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT6630.15487252799618775No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6370.14879909552574902No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT6170.1441272243946423No Hit
GAAACCAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT6040.14109050815942292No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6030.1408569146028676No Hit
CCTGTTTTCATTTACTGTAAGGGGCCCCACAAATTGCTTACATCTATCAA6000.1401561339332016No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAAACTCTATG5820.13595144991520555No Hit
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5810.1357178563586502No Hit
CAATGACATATCCCATGAAGTTAAGGGAATATCCCCATCGTTTTGTTTTG5810.1357178563586502No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5780.1350170756889842No Hit
ATGTCATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTAGAAAAA5610.1310459852275435No Hit
ATCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGC5610.1310459852275435No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTAGAAAAA5460.12754208187921343No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5400.12614052053988142No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5290.12357099141777274No Hit
GGGCTATACCGCCCGCTTCTCCGCCTGTTGTACCGACCGACCACGGGGCG5220.12193583652188539No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA5180.12100146229566404No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC5110.11936630739977669No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG5060.11819833961699999No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT5040.11773115250388934No Hit
ATCCCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG5030.11749755894733399No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4970.11609599760800197No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC4940.11539521693833597No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4920.1149280298252253No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT4870.11376006204244862No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT4870.11376006204244862No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC4860.11352646848589328No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTATGG4800.11212490714656127No Hit
GTATAGCCCCAAGCGGCCCCGGGAGGGGTCGTCCGCGGGATTCAGCGCCG4780.1116577200334506No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4770.11142412647689526No Hit
TGCAGAAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4750.1109569393637846No Hit
GTCTATATGCAGAAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT4640.10838741024167589No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC4630.10815381668512056No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4630.10815381668512056No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTATGGAGTTACTCTCTTTT4620.10792022312856521No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG4620.10792022312856521No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT4610.10768662957200989No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT4530.10581788111956719No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4480.10464991333679052No Hit
ATTTTGTGGTCTATATGCAGAAGGAGTGCGAATCCACACTCCAAAAGACA4420.10324835199745849No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4330.10114600998846048No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC4320.10091241643190513No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC4320.10091241643190513No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)