FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428094
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21660.5059636434988577No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT16520.38589655542941503No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA11520.26909977715174704No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11300.26396071890752965No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC10480.2448060472699921No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10040.23452793078155734No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT9300.21724200759646248No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATT9150.21373810424813242No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCACTCGGCAGT9130.21327091713502175No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA9070.21186935579568972No Hit
GGGCTCTACCGCCCGCTTCTCCGCCTGTTGTACCGACCGACCACGGGGCG8010.18710843880082412No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC7480.17472798030339132No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCGTTTTGTTTTG7100.16585142515428855No Hit
ACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTC7010.16374908314529052No Hit
ATCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGC6960.16258111536251385No Hit
CATTTAAACCCTCACAAAACAAAACGATGGGGATATTCCCTTAACTTCAT6950.1623475218059585No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG6940.16211392824940316No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6790.15861002490107312No Hit
ATGTCATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTAGAAAAA6390.1492662826388597No Hit
GTATAGCCCCAAGCGGCCCCGGGAGGGGTCGTCCGCGGGATTCAGCGCCG6390.1492662826388597No Hit
GGATAATACGGTTTGATCCCTTTATCCAAAGGGAAATATTTAGTAACATT6280.146696753516751No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT6260.14622956640364032No Hit
GGGCTATACCGCCCGCTTCTCCGCCTGTTGTACCGACCGACCACGGGGCG6120.14295925661186562No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5990.13992254037664625No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5940.13875457259386956No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT5900.13782019836764822No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTATGG5890.1375866048110929No Hit
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5780.1350170756889842No Hit
CCTGTTTTCATTTACTGTAAGGGGCCCCACAAATTGCTTACATCTATCAA5740.13408270146276285No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5620.13127957878409882No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTAGAAAAA5570.13011161100132212No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT5490.12824286254887945No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC5460.12754208187921343No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC5450.12730848832265812No Hit
GTCTATATGCAGAAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT5430.12684130120954742No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC5370.12543973987021542No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5230.12216943007844071No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA5190.12123505585221939No Hit
GAAACCAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT5140.12006708806944269No Hit
ATCCCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG5130.11983349451288736No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAAACTCTATG5120.11959990095633202No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATGCTCTGG5000.11679677827766799No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC4960.11586240405144664No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4960.11586240405144664No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT4900.11446084271211462No Hit
CAATGACATATCCCATGAAGTTAAGGGAATATCCCCATCGTTTTGTTTTG4740.11072334580722926No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4730.11048975225067391No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4660.10885459735478656No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4620.10792022312856521No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4550.10628506823267787No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC4420.10324835199745849No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTATGGAGTTACTCTCTTTT4400.10278116488434783No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4380.10231397777123717No Hit
TGCAGAAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4330.10114600998846048No Hit
GTTTACAGGAAGTTTCCTAAAACATAGTTTGATTTTTTCTACAATATGTT4320.10091241643190513No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4310.10067882287534981No Hit
CCTTTTGTCCTCCAACTTGTCCTGGTTATCGTTGGATGTGTCTGCGGCGT4310.10067882287534981No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)