FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397056
Sequences flagged as poor quality0
Sequence length35-151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGAGACTTTGGGGAGAAATGGAGAGGAAATGCTATCATGTACGGT10200.2568907156673114No Hit
CTATACAATTCACCCAGTTAAACTATGCAAGTCATTTCTTAAAATATATT9470.2385053997421019No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCTACAATTCTTTGACATACT7630.1921643294648614No Hit
GCCTAATCCAATGTTTTGAAGATTTTCCCCAGTGTTTTCTTTTAGTGGTT7560.19040135396518376No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6840.1722678916827853No Hit
GTGCTGGGATGAACATGTGAGTGCATGTGTCTTTTTGGGAGAACAGTTTA6260.1576603803997421No Hit
CTATATGTTCAGGGTAATAAGGTTTGATGCCTTTCTCTAAGGGCAAATAT6200.15614925854287556No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAATTATT6170.1553936976144423No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG6150.15488999032882012No Hit
GCTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG5840.14708252740167632No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5770.14531955190199872No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTACGCATGTAT5430.13675652804642166No Hit
ATCCTAAGCTTACCAAATATTTGCCCTTAGAGAAAGGCATCAAACCTTAT5370.13524540618955513No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5310.13373428433268858No Hit
GTGCTGGGAGGATTTTCCATTCTTCAAGTGAAACTGAAATTGAGACTTTT4910.123660138620245No Hit
GTAATAAGGTTTGATGCCTTTCTCTAAGGGCAAATATTTGGTAAGCTTAG4560.11484526112185688No Hit
GTGCTGGCATAAAAACAGAATAGATCAATGGAGCAGAATAGAGAACCCAG4480.11283043197936815No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTATCTCTTCCAAAAGTAAGA4380.11031189555125724No Hit
CATCTACAGTGAACTCATTTTTGACAAAAATTCCAAGAACGTACACTGTG4230.1065340909090909No Hit
GTATACATGCGTACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG4200.10577852998065763No Hit
TCCTTACACCATATAAAAAATCAACTCAAGATGGACTAAAGGCTTAAATA4200.10577852998065763No Hit
GTGCTGGATATTAGACCTTTATTAGATGCATAGTTTGCAAGTATTTGCTC4190.10552667633784654No Hit
ATATAAGACTGCACCCCTATGACCATCTGATCTTCAACAAAGCTGACAAA4160.10477111540941328No Hit
CTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTGCACCTGTC4140.10426740812379111No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG4100.10325999355254674No Hit
GAATAGAGAACCCAGAGACAAACCCATACATCTACAGTGAACTCATTTTT4070.10250443262411348No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)