FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397056
Sequences flagged as poor quality0
Sequence length35-151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGAGACTTTGGGGAGAAATGGAGAGGAAATGCTATCATGTACGGT10510.26469817859445516No Hit
CTATACAATTCACCCAGTTAAACTATGCAAGTCATTTCTTAAAATATATT9900.24933510638297873No Hit
GCCTAATCCAATGTTTTGAAGATTTTCCCCAGTGTTTTCTTTTAGTGGTT8280.20853481624758222No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG7320.1843568665377176No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCTACAATTCTTTGACATACT7270.18309759832366215No Hit
GTGCTGGGATGAACATGTGAGTGCATGTGTCTTTTTGGGAGAACAGTTTA7190.18108276918117344No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6830.17201603803997423No Hit
GCTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG5950.14985291747259832No Hit
CTATATGTTCAGGGTAATAAGGTTTGATGCCTTTCTCTAAGGGCAAATAT5550.13977877176015474No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAATTATT5400.1360009671179884No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTACGCATGTAT5250.13222316247582205No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTATCTCTTCCAAAAGTAAGA5210.1312157479045777No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5110.1286972114764668No Hit
ATCCTAAGCTTACCAAATATTTGCCCTTAGAGAAAGGCATCAAACCTTAT4910.123660138620245No Hit
GTAATAAGGTTTGATGCCTTTCTCTAAGGGCAAATATTTGGTAAGCTTAG4800.12088974854932302No Hit
GTGCTGGATATTAGACCTTTATTAGATGCATAGTTTGCAAGTATTTGCTC4570.11509711476466795No Hit
GTGCTGGCATAAAAACAGAATAGATCAATGGAGCAGAATAGAGAACCCAG4320.10880077369439071No Hit
GTGCTGGGAGGATTTTCCATTCTTCAAGTGAAACTGAAATTGAGACTTTT4310.10854892005157962No Hit
GGGTAATAAGGTTTGATGCCTTTCTCTAAGGGCAAATATTTGGTAAGCTT4170.10502296905222437No Hit
GTATACATGCGTACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG4100.10325999355254674No Hit
CATCTACAGTGAACTCATTTTTGACAAAAATTCCAAGAACGTACACTGTG4070.10250443262411348No Hit
CTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTGCACCTGTC4050.10200072533849129No Hit
GTTCAGGGTAATAAGGTTTGATGCCTTTCTCTAAGGGCAAATATTTGGTA4010.10099331076724695No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG4000.10074145712443586No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)