FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385109
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10990.2853737513275462No Hit
GTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG8710.22616973376368768No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT7590.19708705846916066No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6990.18150705384709265No Hit
GTTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCG6820.17709271920417338No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6110.15865638040139285No Hit
CCTCAAGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6070.1576177134265883No Hit
GTGTAAAAGGGGCAGCAAAGCCCAGAAGACCCACAATTCGTTGACATACT5390.13996037485491122No Hit
CATTTAAACCCTAACAAAACCAAACGTTGGGGCTACTCTCTTAACTTCAT5370.13944104136750896No Hit
ATGTAGGCCCTCTCACAGTTAACGAAAAAAGGAGATTAAAATTAATTATG5110.13268970603127944No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5040.13087203882537152No Hit
GGCCCACATATTGTTGGCATCTATTAATAATGTCTTCCTTCAAATGAATG4890.12697703766985452No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCG4830.12541903720764772No Hit
GTGTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTA4710.12230303628323409No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT4700.12204336953953296No Hit
CTATATAAGAGAGAAACTACACACAGCGCCTCATTTTGTGGGTCACCATA4680.1215240360521307No Hit
CTATTAATAATGTCTTCCTTCAAATGAATGTGAGGAAAGGAGGGAGTTTG4510.11710970140921143No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4380.11373403374109667No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4290.11139703304778646No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAGAGTCCAA4170.10828103212337287No Hit
AGTTAAGAGAGTAGCCCCAACGTTTGGTTTTGTTAGGGTTTAAATGTATA4140.10750203189226945No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA4130.10724236514856832No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4130.10724236514856832No Hit
ATATGTTCCTGTGGCAATGTACCCCAACTCCCAATTACATAGCCCATGAA4100.10646336491746493No Hit
CTCTTAACTTCATGGGCTATGTAATTGGGAGTTGGGGTACATTGCCACAG4090.1062036981737638No Hit
ATTTAGTAAGTTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTC4050.10516503119895924No Hit
TTCTTCTACTGTACCTGTCTTTAATCCAGAGTGGCAAACTCCCTCCTTTC3990.10360703073675245No Hit
CTACTGTACCTGTCTTTAATCCAGAGTGGCAAACTCCCTCCTTTCCTCAC3940.10230869701824678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)