FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386518
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTCGT14070.3640192694777475No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT12520.32391764419768293No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12100.31305139734760085No Hit
TCCACAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA10780.27890033581877166No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10110.26156608489125993No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9410.24345567347445657No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA8990.23258942662437457No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT8900.23026094515649984No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG8740.22612142254694478No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT8480.21939469830641783No Hit
CCCTTACAGTAAATGAAAAAAGAAGACTAAAATTAATTATGCCTGCTAGG8350.21603133618615433No Hit
ATATGTTCCTGTGGCAGTGTTCCCCAACTCCCAATTACATATCCCATGAA8110.20982205227182177No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC7910.20464764900987795No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT7880.20387148852058634No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG7710.19947324574793412No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7550.19533372313837905No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7260.18783083840856052No Hit
GAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAACA7200.1862785174299774No Hit
CTGTAAGGGGCCCCACAAATTGATTACATCTATCAACAATGTCCTCCTGC6590.17049658748104873No Hit
TCCCGATACAGAGCAGTGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA6390.16532218421910494No Hit
GTTGATAGATGTAATCAATTTGTGGGGCCCCTTACAGTAAATGAAAAAAG6240.16144138177264705No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT6060.15678441883689764No Hit
ATCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGC6020.15574953818450887No Hit
ATGGAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA5960.15419721720592572No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCGTCTGACTTCTTTCCTTCTATTCG5850.15135129541185663No Hit
GGTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAA5840.15109257524875944No Hit
ATACAGAGCAGTGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAAGAAGT5700.14747049296539874No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG5660.14643561231301No Hit
ACATAGAGGTGCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG5600.14488329133442687No Hit
GTTTACAGGAAGTTTCCTAAAACACTTTTTGATTTTTTGTACAATATGTT5510.14255480986655214No Hit
ATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTCGTCTGGA5460.14126120905106618No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGCACCTCTATG5430.1404850485617746No Hit
CTCTTTTATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTC5330.1378978469308027No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5320.1376391267677055No Hit
CTCTAAAGCCTCCCGATACAGAGCAGTGGCGGTGTCGAGGAGATCTCGAA5290.13686296627841393No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5250.1358280856260252No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC5150.13324088399505327No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5090.13168856301647014No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5070.13117112269027573No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5070.13117112269027573No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT5030.13013624203788698No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGCGGGTCT5010.1296188017116926No Hit
ACTTGCAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT4910.1270316000807207No Hit
GCTTTATTCTTCTACGGTACCTTGCTTCAATCCTAACTGGCAAACTCCTT4870.12599671942833193No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4780.12366823796045721No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG4740.12263335730806844No Hit
ATATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTG4740.12263335730806844No Hit
CTCTGTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC4660.12056359600329092No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT4650.12030487584019374No Hit
CCCCAACAGTCCCCCATGGGGAGGGATGAACCCTGGCCCGAATGCTCCCA4640.12004615567709653No Hit
GTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT4640.12004615567709653No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4640.12004615567709653No Hit
ATTCTGAGCAGGGCTCACTGTTTCTGAACTGGAGCCACCAGCAGGAAGGT4630.11978743551399934No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4620.11952871535090216No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTTCTTTTTTCATTTAC4610.11926999518780497No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4610.11926999518780497No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4560.11797639437231902No Hit
GGAACATATTGTACAAAAAATCAAAAAGTGTTTTAGGAAACTTCCTGTAA4540.11745895404612462No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4520.11694151371993025No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC4500.11642407339373585No Hit
CCCTTAACTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA4420.11435431208895834No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4340.11228455078418083No Hit
GTTGCAAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4340.11228455078418083No Hit
GAGTTAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGA4310.11150839029488925No Hit
CTCCTACTCCATATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCG4310.11150839029488925No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACTTTTTGATTTTTTGT4310.11150839029488925No Hit
ATATGGAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4290.11099094996869485No Hit
GGTCTATATGGAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATA4290.11099094996869485No Hit
CTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA4260.11021478947940329No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4250.10995606931630608No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA4250.10995606931630608No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA4220.10917990882701452No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTAT4200.10866246850082015No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATCCTGGATCTTC4200.10866246850082015No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA4190.10840374833772294No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT4150.1073688676853342No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA4140.107110147522237No Hit
GATGTAATCAATTTGTGGGGCCCCTTACAGTAAATGAAAAAAGAAGACTA4130.1068514273591398No Hit
CGCTTGATGTATCTGCGGCGTTTTATCATCTTCCTCTGCATCCTGCTGCT4110.10633398703294543No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA4080.10555782654365385No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA4080.10555782654365385No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA4060.10504038621745947No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC3970.10271190474958475No Hit
GATCCTGGATCTTCCAAATTACTTCCCACCCAGGTGGCTAGATTCATCAA3960.10245318458648757No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC3920.1014183039340988No Hit
GCCTAATCATCTCTTGTTCACGTCCTACTGTTCAAGCCTCCAAGCTGTGC3920.1014183039340988No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTTTC3920.1014183039340988No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT3910.10115958377100161No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG3870.10012470311861284No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)