FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386518
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT14030.3629843888253587No Hit
CTTTTATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTCGT14010.36246694849916433No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12070.3122752368583093No Hit
TCCACAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA10210.2641532865222318No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG9560.24733647592091443No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9380.24267951298516496No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA9070.23465918792915205No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT8910.23051966531959703No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8830.2284499040148195No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC8660.22405166124216724No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT8460.21887725798022342No Hit
CCCTTACAGTAAATGAAAAAAGAAGACTAAAATTAATTATGCCTGCTAGG8410.2175836571647375No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8090.20930461194562738No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT7990.20671741031465543No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG7740.20024940623722567No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT7200.1862785174299774No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCGTCTGACTTCTTTCCTTCTATTCG7180.185761077103783No Hit
ATATGTTCCTGTGGCAGTGTTCCCCAACTCCCAATTACATATCCCATGAA7050.18239771498351953No Hit
GAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAACA6720.17385994960131224No Hit
ATCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGC6370.16480474389291055No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6180.15988906079406393No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC6010.15549081802141168No Hit
CTGTAAGGGGCCCCACAAATTGATTACATCTATCAACAATGTCCTCCTGC5800.15005769459637067No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5770.1492815341070791No Hit
GTTGATAGATGTAATCAATTTGTGGGGCCCCTTACAGTAAATGAAAAAAG5720.14798793329159315No Hit
GTTTACAGGAAGTTTCCTAAAACACTTTTTGATTTTTTGTACAATATGTT5670.1466943324761072No Hit
CTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5630.14565945182371842No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG5580.14436585100823246No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA5570.1441071308451353No Hit
ACATAGAGGTGCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG5510.14255480986655214No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT5350.1384152872569971No Hit
CTCTTTTATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTC5320.1376391267677055No Hit
GCCTAATCATCTCTTGTTCACGTCCTACTGTTCAAGCCTCCAAGCTGTGC5260.13608680578912236No Hit
ATGGAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA5200.13453448481053923No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGCACCTCTATG5190.13427576464744204No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCGTCTG5170.13375832432124765No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT5000.1293600815485954No Hit
GGTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAA4970.12858392105930386No Hit
GCTTTATTCTTCTACGGTACCTTGCTTCAATCCTAACTGGCAAACTCCTT4880.1262554395914291No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG4860.12573799926523474No Hit
ATATAAGATACCCGCCTTCCACAGAGTGTGTAAATAATGTCTCGTCTGGA4840.12522055893904036No Hit
GGAACATATTGTACAAAAAATCAAAAAGTGTTTTAGGAAACTTCCTGTAA4830.12496183877594316No Hit
ATATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTG4790.12392695812355439No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4780.12366823796045721No Hit
CTCTGTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC4770.12340951779736002No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC4750.12289207747116565No Hit
GCCTAGTCATCTCTTGTTCACGTCCTACTGTTCAAGCCTCCAAGCTGTGC4740.12263335730806844No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4740.12263335730806844No Hit
GTTGCAAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4730.12237463714497125No Hit
CTCCTACTCCATATAGACCTCCAAATGCCCCTATCTTATCAACACTTCCG4660.12056359600329092No Hit
GGTCTATATGGAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATA4660.12056359600329092No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4630.11978743551399934No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4610.11926999518780497No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4530.11720023388302744No Hit
CCCTTAACTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA4520.11694151371993025No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTTCTTTTTTCATTTAC4500.11642407339373585No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4490.11616535323063867No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4470.11564791290444429No Hit
GAGTTAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGA4470.11564791290444429No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4420.11435431208895834No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA4410.11409559192586115No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGCGGGTCT4390.11357815159966676No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC4370.11306071127347239No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4330.11202583062108362No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4300.11124967013179206No Hit
GTTCACGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAG4250.10995606931630608No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4230.10943862899011171No Hit
ATTCTGAGCAGGGCTCACTGTTTCTGAACTGGAGCCACCAGCAGGAAGGT4220.10917990882701452No Hit
CCCCAACAGTCCCCCATGGGGAGGGATGAACCCTGGCCCGAATGCTCCCA4210.10892118866391734No Hit
GATGTAATCAATTTGTGGGGCCCCTTACAGTAAATGAAAAAAGAAGACTA4200.10866246850082015No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA4180.10814502817462576No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACTTTTTGATTTTTTGT4170.10788630801152857No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4140.107110147522237No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4120.10659270719604262No Hit
AGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTACGTCC4090.10581654670675103No Hit
ATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA4090.10581654670675103No Hit
ATATGGAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4060.10504038621745947No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC4050.10478166605436229No Hit
TTCAAGAACAGTTTCTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACA4050.10478166605436229No Hit
GTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT4040.1045229458912651No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTAT4020.10400550556507071No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4010.10374678540197352No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTTAGATCTTCTGCGAC4010.10374678540197352No Hit
ACTTGCAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT3980.10297062491268194No Hit
GCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA3980.10297062491268194No Hit
CAAATCTTTCTGTCCCCAATCCCCTGGGATTCTTCCCCGATCATCAGTTG3960.10245318458648757No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATCCTGGATCTTC3960.10245318458648757No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA3950.10219446442339038No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT3910.10115958377100161No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG3910.10115958377100161No Hit
TCCCGATACAGAGCAGTGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA3880.10038342328171003No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC3870.10012470311861284No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)