FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346025
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCCACACTGCGTGAAAGGGGCTGCAAACCCCAAAAGACCCACAATTCG14300.4132649375045156No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10860.3138501553355971No Hit
TCCAAAGAGTGTGTAAATAGTGTATCGTCTGGAAGTAATGATTAACCACA10480.3028682898634492No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAGTGTATCGT10280.2970883606675818No Hit
GTGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC9890.2858174987356405No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9050.2615417961129976No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC8600.2485369554222961No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7490.21645834838523226No Hit
CTATTATGGTTTCACATTTCCTGTCTTTCTTTTGGGAGAGAAACTGTTCT7450.21530236254605883No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACCTT7240.2092334368903981No Hit
GAAACCAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAAGGT7220.20865544397081137No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7220.20865544397081137No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6590.1904486670038292No Hit
GTGTAAATAGTGTATCGTCTGGAAGTAATGATTAACCACATGCTCTGGAT6510.18813669532548227No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTATGGTTTCACATTTCCT6270.18120078029044145No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6100.17628784047395418No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACACCCAATTACATATCCCATGAA6060.17513185463478073No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5990.17310887941622716No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5890.17021891481829346No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGAAAGA5690.16443898562242612No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5270.15230113431110467No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5100.14738819449461746No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5090.14709919803482407No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT5060.14623220865544398No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACACCCGTG4940.14276425113792357No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4940.14276425113792357No Hit
TTATTATCCAGAGCATGTGGTTAATCATTACTTCCAGACGATACACTATT4910.14189726175854345No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4800.13871830070081642No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC4770.1378513113214363No Hit
CCATAATAGTTGTCTGATTTTTAGGCCCATATTAACGTTGACATAGCTGA4770.1378513113214363No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAA4610.13322736796474244No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAGTGTATCGTCTGGA4600.13293837150494908No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4580.13236037858536234No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4510.13033740336680877No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAGTGTATC4510.13033740336680877No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4410.12744743876887507No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT4400.12715844230908172No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4370.12629145292970162No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4330.12513546709052814No Hit
CCTGTTTTCATTTACTGTCAGGGGCCCCACAAATTGCTGACATCTATCAA4320.12484647063073478No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC4290.12397948125135466No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG4230.12224550249259447No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4180.12080052019362764No Hit
ATGTAATTGGGTGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA4130.11935553789466079No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4030.11646557329672712No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA3990.11530958745755364No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT3950.11415360161838017No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3940.1138646051585868No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC3920.11328661223900006No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT3880.1121306263998266No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACACTGCGTGAAA3760.1086626688823062No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG3660.10577270428437252No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA3620.10461671844519906No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC3590.10374972906581895No Hit
CTATTTACACACTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCA3560.10288273968643884No Hit
ATCCCAAGGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCTTAT3540.10230474676685211No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA3530.10201575030705874No Hit
CCCAAAGTCAGGGCCCTGTACTTTCCTGCTGGTGGCTCCAGTTCAGGAAC3500.10114876092767863No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)