FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346025
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC11040.31905209161187775No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAGTGTATCGT10320.2982443465067553No Hit
TATCCACACTGCGTGAAAGGGGCTGCAAACCCCAAAAGACCCACAATTCG9560.27628061556245936No Hit
TCCAAAGAGTGTGTAAATAGTGTATCGTCTGGAAGTAATGATTAACCACA9070.26211978903258437No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8580.24795896250270932No Hit
GTGTAAATAGTGTATCGTCTGGAAGTAATGATTAACCACATGCTCTGGAT8050.23264215013366085No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA7460.2155913590058522No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7370.21299039086771188No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6910.19969655371721695No Hit
CTATTATGGTTTCACATTTCCTGTCTTTCTTTTGGGAGAGAAACTGTTCT6790.19622859619969654No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACCTT6750.1950726103605231No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT5830.16848493605953327No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGAAAGA5670.1638609927028394No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5470.15808106350697204No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC5440.15721407412759195No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT5420.1566360812080052No Hit
GAAACCAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAAGGT5410.15634708474821182No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5280.15259013077089806No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5270.15230113431110467No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACACCCGTG4890.14131926883895674No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTATGGTTTCACATTTCCT4870.14074127591937No Hit
TTATTATCCAGAGCATGTGGTTAATCATTACTTCCAGACGATACACTATT4660.13467235026370927No Hit
CTATTTACACACTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCA4640.13409435734412253No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4590.1326493750451557No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA4480.12947041398742865No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC4460.12889242106784193No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACACCCAATTACATATCCCATGAA4380.12658044938949498No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA4280.12369048479156129No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT4250.12282349541218121No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4230.12224550249259447No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAA4020.11617657683693375No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC3970.11473159453796691No Hit
CCATAATAGTTGTCTGATTTTTAGGCCCATATTAACGTTGACATAGCTGA3910.1129976157792067No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAGTGTATCGTCTGGA3910.1129976157792067No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG3890.11241962285961997No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT3820.11039664764106641No Hit
GTGTGTAAATAGTGTATCGTCTGGAAGTAATGATTAACCACATGCTCTGG3810.11010765118127303No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA3710.10721768658333934No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3690.10663969366375263No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT3660.10577270428437252No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACACTGCGTGAAA3650.10548370782457915No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAGTGTATC3640.10519471136478578No Hit
GTTTTATCCCAAGGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC3630.1049057149049924No Hit
GCTTAGGGCTCTACCGCCCGCTTCTCCGCCTGCTGTACCGACCGACCACG3620.10461671844519906No Hit
ATGTAATTGGGTGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA3590.10374972906581895No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT3580.10346073260602558No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACGG3570.1031717361462322No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3550.10259374322664548No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)