FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441314
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG26030.5898294638284759No Hit
ATCCTAAACTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT25230.571701781497981No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19070.4321186275531708No Hit
ATCCGAAACTTACTAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT15590.3532632094155182No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG15250.34555894442505786No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG14590.33060360650239967No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT14220.3222195534245458No Hit
GTTTAGGATAGAACCTAGCAGGCATAATTAGTTTTAATCTCCTTTTTTCG11550.26171841364651927No Hit
GTTTCGGATAGAACCTAGCAGGCATAATTAGTTTTAATCTCCTTTTTTCG11500.26058543350086333No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10360.23475348617990818No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT9660.21889176414072517No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT9520.2157194197328886No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8810.19963110166457443No Hit
CTCTTATATAAAATACCCGCCTTCCATAGAGTATGTAAATAATGCCTAGT8620.19532577711108193No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAACTAATTATGCCTGCTAGG8390.19011406844106463No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC8340.18898108829540872No Hit
CTCTCAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGATTG8130.1842225716836538No Hit
CTTATATAAAATACCCGCCTTCCATAGAGTATGTAAATAATGCCTAGTTT8120.18399597565452264No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7810.17697149875145587No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAACTAA7710.17470553846014403No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA7670.17379915434361928No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT7660.1735725583144881No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG7160.16224275685792883No Hit
GAATGCAGGGTCCAACTGGTGATCGGGAAAGAATCCCAGAGGATTGGGAA6870.15567147201312445No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6690.1515927434887631No Hit
CTCTATGGAAGGCGGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC6600.14955337922658243No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT6460.14638103481874584No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6410.14524805467308993No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC6320.14320869041090925No Hit
CCATAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGT6310.14298209438177806No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA6220.14094273011959738No Hit
ATATAAAATACCCGCCTTCCATAGAGTATGTAAATAATGCCTAGTTTGGA6120.13867676982828553No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6030.13663740556610485No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA5990.1357310214495801No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA5990.1357310214495801No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC5920.1341448492456618No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG5840.13233208101261232No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT5730.12983952469216928No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC5620.12734696837172624No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGA5570.12621398822607033No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTCTCCATGC5260.11918951132300357No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA5210.11805653117734766No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT5210.11805653117734766No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTAGTAAGTTT5200.11782993514821646No Hit
TTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTATTATCCTGAA5170.1171501470608229No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5140.11647035897342935No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT5100.11556397485690462No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5090.11533737882777342No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5050.11443099471124868No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGTGAGG4940.11193843839080564No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4900.1110320542742809No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC4840.10967247809949379No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC4820.10921928604123142No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4800.10876609398296905No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA4720.10695332574991954No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4700.10650013369165717No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG4630.10491396148773889No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4560.1033277892838206No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG4500.10196821310903348No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC4500.10196821310903348No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT4490.10174161707990229No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAACTAATTATG4490.10174161707990229No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4450.10083523296337754No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT4450.10083523296337754No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGGAT4440.10060863693424638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)