FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441314
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG25270.5726081656145058No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG19840.449566521796272No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19130.43347820372795787No Hit
ATCCTAAACTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT19070.4321186275531708No Hit
ATCCGAAACTTACTAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT12590.2852844006761625No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG12370.2802992880352765No Hit
GTTTCGGATAGAACCTAGCAGGCATAATTAGTTTTAATCTCCTTTTTTCG12170.27576736745265273No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT12020.272368427015685No Hit
GTTTAGGATAGAACCTAGCAGGCATAATTAGTTTTAATCTCCTTTTTTCG11380.2578662811512891No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8990.2037098301889358No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT8890.20144386989762392No Hit
CTCTCAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGATTG8720.19759173740239375No Hit
CTCTTATATAAAATACCCGCCTTCCATAGAGTATGTAAATAATGCCTAGT8390.19011406844106463No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8050.18240980345060434No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT7860.1781044788971118No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC7770.17606511463493113No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAACTAATTATGCCTGCTAGG7620.17266617419796335No Hit
CTTATATAAAATACCCGCCTTCCATAGAGTATGTAAATAATGCCTAGTTT7280.16496190920750306No Hit
GAATGCAGGGTCCAACTGGTGATCGGGAAAGAATCCCAGAGGATTGGGAA7120.16133637274140408No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6940.15725764421704275No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG6670.15113955143050073No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6300.14275549835264686No Hit
CTCTATGGAAGGCGGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC6240.14139592217785973No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAACTAA6200.14048953806133502No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA6140.1391299618865479No Hit
CCATAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGT6110.13845017379915436No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT6080.13777038571176078No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC6060.13731719365349843No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6050.13709059762436723No Hit
ATATAAAATACCCGCCTTCCATAGAGTATGTAAATAATGCCTAGTTTGGA6040.13686400159523604No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA6010.1361842135078425No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT5920.1341448492456618No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA5870.1330118691000059No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC5860.1327852730708747No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC5750.13029271675043166No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5640.12780016042998862No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC5620.12734696837172624No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT5600.12689377631346388No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG5210.11805653117734766No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA5140.11647035897342935No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4740.10740651780818194No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG4710.10672672972078838No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4700.10650013369165717No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4680.10604694163339481No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAACTAATTATG4680.10604694163339481No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTAGTAAGTTT4680.10604694163339481No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4660.10559374957513244No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGGAT4660.10559374957513244No Hit
AGCCCAGAAGCCCCACAATTCGTTGACATACTTTCCAATCAATAGGTCTA4620.1046873654586077No Hit
CTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGATTG4610.10446076942947652No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT4580.10378098134208297No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4580.10378098134208297No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4510.10219480913816466No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4480.1015150210507711No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4430.10038204090511518No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)