FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences368318
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12710.3450822387176299No Hit
GTTAATATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC12190.33096400393138536No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11340.30788612014617806No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9840.26716044287816504No Hit
ACTATATGCTCTGGATAATAGGGTTTGATCCCTTTATCTAAGGGCAAATA9380.25467123518264106No Hit
ACTACATGCTCTGGATAATAGGGTTTGATCCCTTTATCTAAGGGCAAATA9360.25412822615240094No Hit
ATATGGAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA8510.23105034236719357No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT7910.2147600714599884No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT7880.2139455579146281No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7300.19819829603766312No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT6930.1881526289782199No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTGTCTCCCGAAAGTAAGA6510.17674943934317627No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTTTTCAAGCCTCCAAGCTGTGC6210.16860430388957368No Hit
CCCTAACAGTCCCCCATGGGGAGGGATGAACCCTGGCCCGAATGCTCCCA6090.16534624970813264No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA6010.16317421358717196No Hit
GAATATTAACATGGAGATTGCCGAGATTGAGATCTTCTGCGACGCGGCGA5880.15964465489061083No Hit
CGTATAAAGAATTTGGAGCTTCTGTGGAATTACTCTCTTTTTTGCCTTCT5750.15611509619404973No Hit
CCTCCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAAC5580.15149951943700823No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5550.150685005891648No Hit
GGATAATAGGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT5520.14987049234628771No Hit
CCTGTAAACAGACCCATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT5440.147698456225327No Hit
GAATACTAACATGGAGATTGCCGAGATTGAGATCTTCTGCGACGCGGCGA5440.147698456225327No Hit
GATTTAGGTCCCAATCTGGATTTCCTGAGTTGGCTTTGAATGCTGGGTCC5380.14606942913460652No Hit
ATATTAACGTTGACATAGCTGACTACTGCTTCCCTGGATGTTGGATCGTC5370.14579792461948643No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5310.1441688975287659No Hit
CCCCAACACACAATAGCTTGCCTGAGTGCCGTATGGTGAGCTGAACAATG5290.14362588849852573No Hit
ATCCAACATCCAGGGAAGCAGTAGTCAGCTATGTCAACGTTAATATGGGC5280.14335438398340564No Hit
CGTAAAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTTGCTTG5260.14281137495316548No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC5150.13982482528684453No Hit
GCATATAGTTAATCATTACTTCCAGACGAGACATTATTTACACACTCTTT5050.13710978013564368No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG5000.13575225756004322No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTCGGAAACTTCCTGTAA4990.13548075304492313No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG4740.12869314016692096No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC4670.12679260856108038No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTTTTCAAGCCTCCAAGCTGTGC4650.1262495995308402No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4520.12272004083427906No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTC4500.1221770318040389No Hit
GCTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGG4400.11946198665283804No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4340.1178329595621175No Hit
GTTTACAGGAAGTTTCCGAAAACACATTTTGATTTTTTGTACAATATGTT4300.11674694150163717No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCTAACAGTCCCCCATG4220.11457490538067648No Hit
CATCATTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG4140.11240286925971578No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACT4110.11158835571435553No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTATA4090.11104534668411536No Hit
ACCTCAATCAGCACAAGGACAACTGGCCGGACGCCAACAAGGTGGGAGTG4060.1102308331387551No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4050.10995932862363503No Hit
CTTTATATGCGTGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACT4010.10887331056315466No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATTA3980.10805879701779442No Hit
CCTTTATACCGCTGTTACCAATTTTCTTCTGTCTTTGGGTGTACATTTAA3870.10507224735147346No Hit
CTGTTAGGGTGGAGCCCTCAGGCTCAGGGCATACTCACAACTGTGCCAGC3840.10425773380611318No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA3830.1039862292909931No Hit
CACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCT3830.1039862292909931No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTCGGGAGACAAACTGTTCT3830.1039862292909931No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC3810.10344322026075294No Hit
ATATGTTCCTGTGGCAGCGTGCCCCAACCCCCAATTACATATCCCATGAA3770.1023572022002726No Hit
TTCAAGAACAGTTTGTCTCCCGAAAGTAAGACAGGAAATGTGAAACCACA3760.10208569768515251No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCGTGTATACAAGCAAAACAG3730.10127118413979223No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)