FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002489468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287494
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12350.42957418241772No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT7720.2685273431793359No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT7630.2653968430645509No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7610.26470117637237645No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7220.25113567587497476No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA7070.24591817568366645No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA6160.21426534118972917No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT5650.19652584053928085No Hit
GGAAAGTACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGT5610.19513450715493194No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA5410.18817784023318748No Hit
CTCCAAAAGACACCAAATATTCAAGAACAACTTCTCTCCCAAAAGTAAGA5280.18365600673405358No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG4990.17356883969752412No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA4230.1471335053948952No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT4160.14469867197228464No Hit
GTACCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA4040.14052467181923797No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGGTATTCCCTTAACTTCAT4030.14017683847315074No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT3840.13356800489749351No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG3820.13287233820531907No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC3630.12626350462966185No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA3530.12278517116878962No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA3530.12278517116878962No Hit
TCCTCAGGCCATGCGGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG3420.11895900436183016No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATAACCCATGAA3380.11756767097748128No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT3370.11721983763139404No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA3240.11269800413226015No Hit
CCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT3220.1120023374400857No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCGGTGGAA3160.10991533736356238No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG2990.10400217048007958No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT2940.10226300374964346No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC2890.10052383701920735No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT2880.10017600367312014No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)