FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002489469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002489469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287494
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12340.4292263490716328No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT7140.248353009106277No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6540.22748300834104362No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6410.22296117484190975No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC5970.20765650761407195No Hit
CTCCAAAAGACACCAAATATTCAAGAACAACTTCTCTCCCAAAAGTAAGA5300.18435167342622802No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG4980.1732210063514369No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA4760.165568672737518No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA4750.16522083939143076No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT4700.16348167266099467No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA4670.162438172622733No Hit
GGAAAGTACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGT4430.15409017231663966No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG4150.14435083862619744No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4030.14017683847315074No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT3990.13878550508880186No Hit
GTACCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA3750.13043750478270852No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC3410.11861117101574292No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA3220.1120023374400857No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATAACCCATGAA3120.10852400397921348No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGGTATTCCCTTAACTTCAT3110.10817617063312626No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA3040.10574133721051569No Hit
TCCTCAGGCCATGCGGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG2930.10191517040355626No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG2920.10156733705746902No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC2890.10052383701920735No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)